Development of Basmati rice genotypes with resistance to both bacterial blight and blast diseases using marker assisted restricted backcross breeding

Abstract

Marker assisted backcross breeding (MABB) is aimed at introgression of trait(s) into a popular variety to augment specific trait(s) in an otherwise popular variety. While MABB can improve a variety with respect to introgressed trait(s), it offers very little scope for improvement of other traits. Marker assisted restricted backcross breeding (MARBB) is an alternative which can help in identifying transgressive segregants especially, when the donor parent is an elite genotype with several desirable traits. In the present study, restricted backcrossing followed by pedigree selection was used for the development of improved genotypes of Basmati rice with BB and blast diseases using an early maturing Basmati rice variety, Pusa Basmati 1509 as recurrent parent and an elite restorer line, Pusa 1790 as donor. Foreground selection for xa13, Xa21, Pi2 and Pi54 in the backcross progenies was combined with phenotypic selection for agronomic and grain quality traits to ensure premium Basmati grain quality in the progenies. Multilocation yield trial was conducted to evaluate the performance of the improved Basmati rice genotypes with both BB and blast resistance.

Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

“…The shift in flowering duration under stress conditions in the present experiment indicates that water stress at the initiation of the reproductive stage affected flowering time. Similar results were reported by earlier studies [15,23,45,[49][50][51][52][53][54][55][56]. Bernier et al [9] and Venuprasad et al [16] reported that qDTY12.1 and qDTY3.1 influenced both grain yield and days to flowering under stress conditions, evincing that genes present within the QTLs are likely to be allied with early flowering.…”

“…The performance of the NILs under unstressed situations proved that they recovered the maximum agronomic and grain quality attributes of Pusa 44 during the selection process. As successfully reported earlier [50][51][52][53], a stringent phenotypic selection along with marker-based background selection ensured an RPG recovery ranging between 98.6 and 99.4% after three successive backcrosses. This was further apparent from the recombinant selection data obtained later in the selection cycle, during the BC 3 F 4 stage, that indicated complete recovery of carrier chromosomes.…”

Introgression of qDTY1.1 Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding

“…Therefore, marker assisted backcross breeding (MABB) was proven to be an efficient strategy for incorporation of desired trait associated genes/QTLs in numerous prominent rice varieties [21,42,43]. MABB along with stringent phenotypic selection was successfully employed for Basmati rice improvement for different biotic stresses [26,30,[44][45][46][47][48]. MABB is also proven to a superior method for QTLs transfer into desired cultivar/variety for different abiotic stresses as well, such as drought.…”

Introgression of qDTY1.1 Governing Reproductive Stage Drought Tolerance Into an Elite Rice Variety ‘Pusa Basmati 1’ Through Marker Assisted Backcross Breeding

“…Therefore, marker assisted backcross breeding (MABB) was proven to be an efficient strategy for incorporation of desired trait associated genes/ QTLs in numerous prominent rice varieties [21,41,42]. MABB along with stringent phenotypic selection was successfully employed for Basmati rice improvement for different biotic stresses [43,44,45,25,46,29,47]. MABB is also proven to a superior method for QTLs transfer into desired cultivar/variety for different abiotic stresses as well, such as drought.…”