Development of biodegradable zein-based bilayer coatings for drug-eluting stents

Lenzuni, Martina; Suarato, Giulia; Miele, Dalila; Carzino, Riccardo; Ruggeri, Marco; Bertorelli, Rosalia; Sandri, Giuseppina; Athanassiou, Athanassia

doi:10.1039/d1ra03748j

Cited by 18 publications

(14 citation statements)

References 51 publications

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“…Compared to the static experiments reported in our previous work, 21 both zein degradation and rutin release were found to be faster and more continuous when operating under dynamic conditions. The most likely explanation of these outcomes is that the constant liquid flow resulted in a more rapid infiltration of water within the polymer matrix and, as a consequence, in a constant removal of the drug/ polymer top surface (i.e., maintenance of the continuous concentration gradient and avoidance of the risk of medium saturation), such that the polymer degradation is accelerated.…”

Section: Drug Release Kinetics and Mathematical Modelscontrasting

confidence: 76%

“…Zein is a protein-based natural polymer still little investigated for its biomedical applications but has already shown a slow degradation rate in aqueous environments, especially compared to other naturally-derived polymers. 21,45,46 To investigate the degradation kinetics of the polymer composite coating under dynamic conditions, the ZR-coated channels were exposed to two aqueous SBFs at a continuous flow rate of 500 μL min −1 for up to 6 hours. In the first stage of the degradation kinetics, the polymer release appears more abrupt, while the zein mass remaining on the channels continues to decrease at the following stages but at slower rates, consequently diffusing into the release media (Fig.…”

Section: Matrix Degradation Studiesmentioning

confidence: 99%

“…Experimental results revealed that ZR-coated chambers were partially hydrophilic as they display a mean water contact angle of 60.5°(Table 1), which is in accordance with the previously obtained values for zein-based substrates. 21,44…”

Section: Characterization Of the Zein-based Coated Channelsmentioning

confidence: 99%

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Dynamic investigation of zein-based degradable and hemocompatible coatings for drug-eluting stents: a microfluidic approach

et al. 2023

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Section: Drug Release Kinetics and Mathematical Modelscontrasting

confidence: 76%

Section: Matrix Degradation Studiesmentioning

confidence: 99%

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Dynamic investigation of zein-based degradable and hemocompatible coatings for drug-eluting stents: a microfluidic approach

et al. 2023

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“…Zein proteins have found wide applications as excipients in the production of inks [ 9 ], adhesives [ 10 ], water resistant paper coating [ 11 , 12 ], plastic-free replacement [ 13 ], encapsulated foods [ 14 ], and a controlled release matrix of active medical components [ 15 , 16 , 17 , 18 ]. Moreover, among various protein sources, zein (the primary storage protein in corn endosperm) has been extensively studied for its capacity to create innovative polymeric films, a feature we want to explore in the future [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction

Darie-Ion

Jayathirtha

Hitruc³

et al. 2021

Biomolecules

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Zein is a type of prolamin storage protein that has a variety of biomedical and industrial applications. Due to the considerable genetic variability and polyploidity of the starting material, as well as the extraction methods used, the characterization of the protein composition of zein requires a combination of different analytical processes. Therefore, we combined modern analytical methods such as mass spectrometry (MS), Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), atomic force microscopy (AFM), or Fourier transform infrared spectroscopy–attenuated total reflectance (FTIR-ATR) for a better characterization of the extracted zein. In this study, we present an enhanced eco-friendly extraction method, including grinding and sieving corn seeds, for prolamins proteins using an ultrasonic extraction methodology. The use of an ultrasonic homogenizer, 65% ethanol extraction buffer, and 710 µm maize granulation yielded the highest protein extraction from all experimental conditions we employed. An SDS PAGE analysis of the extracted zein protein mainly revealed two intense bands of approximatively 20 and 23 kDa, suggesting that the extracted zein was mostly α-zein monomer. Additionally, MS analysis revealed as a main component the α-zein PMS2 (Uniprot accession no. P24450) type protein in the maize flour extract. Moreover, AFM studies show that extracting zein with a 65% ethanol and a 710 µm granulation yields a homogeneous content that could allow these proteins to be employed in future medical applications. This research leads to a better understanding of zeins content critical for developing new applications of zein in food and pharmaceutical industries, such as biocompatible medical vehicles based on polyplexes complex nanoparticles of zein with antimicrobial or drug delivery properties.

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“…1 The metallic stent with specific geometry was introduced as a treatment option for CVD 2,3 but the damage caused to arterial wall triggers the inflammatory reactions within the body causing the development of in-stent restenosis in 30% of the patients. 4–7 Therefore, approaches for the treatment of CVDs have been accelerated for past decades. After balloon angioplasty and BMS, DES is brought third revolution in minimal invasive cardiology and has attained a position of gold standard in PCI.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and in vitro evaluation of natural drug loaded polymeric films for cardiovascular applications

Ghafoor

Ali

2022

Journal of Bioactive and Compatible Polymers

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Drug eluting stents (DES) can efficiently reduce the atherosclerosis and restenosis issues of coronary artery as compared to bare metal stents due to the presence of pharmaceutically active agent on their surface. Nevertheless, the arising safety concerns of DES such as delayed healing and late in stent restenosis and thrombus, has stirred the research efforts to improve the outcomes of the DES. In this connection, attention is being shifted from the use of synthetic drug to natural drug for DES. In the present work, natural compound loaded polymeric films were synthesized and their antioxidant and anticoagulation capabilities were assessed through in vitro testing. The potential of the drug loaded polymeric films to curb the production of free radicals was evaluated by carrying out antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The in vitro platelet adhesion was investigated through static platelet adhesion test while effect of synthesized films on intrinsic coagulation pathway was investigated through activated partially thromboplastin time (APTT). Moreover, to further evaluate the blood compatibility of the developed drug loaded films, in vitro hemolytic and anti-thrombolytic assays were carried out. The obtained results indicated that, incorporating herbal compounds such as ginger, magnolol and curcumin, in polymeric matrix (PVA) has significantly improved the blood compatibility of the polymeric films. Hence, it can be concluded that the synthesized drug loaded polymeric films have the potential capability to be used as a potential coating material for coating biomedical implants with good anticoagulation and antioxidant property to cater the cardiovascular issues such as atherosclerosis, restenosis and thrombus formation.

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Development of biodegradable zein-based bilayer coatings for drug-eluting stents

Abstract: Zein-based biodegradable bilayer coatings were successfully prepared and characterized. Release profiles, antioxidant potential, and biocompatibility were investigated, aiming for more sustainable coatings for drug-eluting stents.

Cited by 18 publications

References 51 publications

Dynamic investigation of zein-based degradable and hemocompatible coatings for drug-eluting stents: a microfluidic approach

Dynamic investigation of zein-based degradable and hemocompatible coatings for drug-eluting stents: a microfluidic approach

A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction

Synthesis and in vitro evaluation of natural drug loaded polymeric films for cardiovascular applications

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