Development of bovine–ovine interspecies cloned embryos and mitochondria segregation in blastomeres during preimplantation

Shi, Hua; Zhang, Yong; Song, Kai; Song, Jimei; Zhang, Zhipeng; Zhang, Lin; Zhang, Chi; Cao, Junwei; Ma, Libing

doi:10.1016/j.anireprosci.2007.03.002

Cited by 37 publications

(45 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA polymerase I function and rDNA transcription, in ovine-bovine iSCNT embryos. In contrast to Hua et al (2008), who obtained almost 25% of BLs with 117G13 cells/BL on day 6, we were not able to obtain morulae or BLs of such ovine-bovine iSCNT embryos. The development of our iSCNT embryos, as well as that of hybrid embryos between bovine oocytes and ram sperm (Slavik et al 1997), was blocked at the time of EGA.…”

Section: Discussionmentioning

confidence: 41%

“…SOF (synthetic oviduct fluid) in vitro embryo culture medium that is equally efficient for bovine and porcine IVF/NT preimplantation development not only failed to support the development but also was not able to initiate EGA in porcine-bovine iSCNT embryos (Lagutina et al 2010). We were not able to obtain ovine-bovine iSCNT BLs in SOF (Hua et al 2008) despite nucleoli formation as a sign of EGA. Undoubtedly, there is still a wide field for investigation of in vitro maturation and embryo culture medium effects on the EGA in iSCNT embryos.…”

Section: Discussionmentioning

confidence: 56%

See 1 more Smart Citation

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

View full text Add to dashboard Cite

The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has been achieved in closely related species. The analyses of embryonic gene activity in iSCNT embryos of different species combinations have revealed the existence of significant aberrations in expression of housekeeping genes and genes dependent on the major embryonic genome activation (EGA). However, there are many studies with successful blastocyst (BL) development of iSCNT embryos derived from donor cells and oocytes of animal species with distant taxonomical relations (inter-family/inter-class) that should indicate proper EGA at least in terms of RNA polymerase I activation, nucleoli formation, and activation of genes engaged in morula and BL formation. We investigated the ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species. In iSCNT embryos, nucleoli precursor bodies originate from the oocyte, while most proteins engaged in the formation of mature nucleoli should be transcribed from genes de novo in the donor nucleus at the time of EGA. Thus, the success of nucleoli formation depends on species compatibility of many components of this complex process. We demonstrate that the time and cell stage of nucleoli formation are under the control of recipient ooplasm. Oocytes of the studied species possess different abilities to support nucleoli formation. Formation of nucleoli, which is a complex but small part of the whole process of EGA, is essential but not absolutely sufficient for the development of iSCNT embryos to the morula and BL stages.

show abstract

Section: Discussionmentioning

confidence: 41%

Section: Discussionmentioning

confidence: 56%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

View full text Add to dashboard Cite

show abstract

“…Persistence of donor mtDNA is observed in SCNT (Do et al 2002;Bowles et al 2008) and interspecies SCNT (iSCNT; (Yang et al 2003;Hua et al 2008;Ma et al 2008) and ranges from 0% to 69% during preimplantation development. Transmission of mtDNA to cloned offspring is extremely important for the production of interspecies SCNT embryos and the derivation of patient specific embryonic stem cells (ESCs; (St. John and Lovell-Badge 2007).…”

Section: Transmission Of Mtdna Following Scnt: Homoplasmy or Heteroplmentioning

confidence: 99%

Role of mitochondrial DNA replication during differentiation of reprogrammed stem cells

Kelly

John²

2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Mitochondrial DNA (mtDNA) is a 16.6 kb genome that encodes for 13 of the 100+ subunits of the electron transfer chain (ETC), whilst the other subunits are encoded by chromosomal DNA. The ETC is responsible for the generation of the majority of cellular ATP through the process of oxidative phosphorylation (OXPHOS). mtDNA is normally inherited from the population present in the mature oocyte just prior to fertilisation. However, following somatic cell nuclear transfer (SCNT), mtDNA can be transmitted from both the donor cell and the recipient oocyte. This heteroplasmic transmission of mtDNA is a random event and does not appear to be related to the amount of mtDNA contributed by the donor cell. The distribution of mtDNA is randomly segregated between blastomeres and differentiating tissues, and therefore the mtDNA complement transmitted to offspring tissue cannot be predicted. mtDNA divergence between the cytoplast and the donor cell in intra-and inter-specific crosses favours a slightly more diverse mtDNA haplotype. However, this is limited as interspecies SCNT (iSCNT) genetic divergence contributes to developmental failure. SCNT embryos demonstrate a plethora of aberrantly reprogrammed characteristics including the uncoordinated regulation of the mtDNA replication factors. This results in increased mtDNA copy number during preimplantation development and propagates the replication of donor cell mtDNA. These failures are likely to be a consequence of incompatible nuclear-and mtDNA -encoded proteins interacting within the ETC thus reducing ATP production. The outcomes would be similar to the severely debilitating or even fatal mtDNA diseases associated with genetic rearrangements to mtDNA or mtDNA depletion type syndromes and have serious implications for any form of karyoplast transfer approach. The only method to overcome the problems of heteroplasmy in SCNT embryos is to completely deplete the donor cell of its mtDNA prior to SCNT.

show abstract

“…Additionally, our results illustrated that exogeous interspecies/intergeneric mtDNA injected into oocytes were not selectively destroyed; indeed, they were maintained throughout the early development. In iSCNT embryos, constant copy numbers of donor somatic cell mtDNA from the 1-cell to the 8-cell stage were also found in water buffalo-bovine [18], sheep-bovine [20], cat-bovine [21], goat-sheep [22], and macaque-rabbit [17] arrested embryos. Sperm mitochondria destruction is induced by a process related to the ubiquitin-proteasome pathway in fertilization [30,41].…”

Section: Takeda Et Al 328mentioning

confidence: 99%

Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells

Takeda

Srirattana

Matsukawa

et al. 2012

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. Interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. In the present study, intergeneric buffalo (Bubalus bubalis) mitochondria (WB-mt) or interspecies murine (Mus spretus) mitochondria (M-mt) were injected into bovine (Bos taurus) oocytes, and the subsequent embryonic development was characterized. Fibroblast mitochondria (WB-mt or M-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte activation by a combination of electrical stimulation and 6-dimethylaminopurine treatment. After seven days of culture, embryo development was evaluated. The copy number of specific mtDNA populations (introduced and native mtDNA) from heteroplasmic oocytes was estimated using real-time PCR. The results illustrated that oocytes injected with either WB-mt or M-mt can develop to the blastocyst stage (20.6% and 19.6%). Cleavage division rates and development to the morula stage in oocytes injected with WB-mt were lower (76.2% and 45.9%, respectively) in comparison with uninjected oocytes (89.2% and 59.1%, respectively) (P<0.05). However, no differences were found in comparing M-mt injected oocytes and controls (P>0.05). An increase in bovine mtDNA copy number was observed at the expanded blastocyst stage of injected embryos (P<0.01), while the number of injected mtDNA was stable throughout development. This study demonstrates that interspecies/intergeneric mitochondrial injected bovine oocytes have the ability to develop to the blastocyst stage after parthenogenetic activation and that injected mtDNA was neither selectively destroyed nor enhanced through development. Moreover, injected intergeneric mitochondria had a demonstrated influence on bovine parthenogenetic development and mtDNA replication. Key words: Bovine oocyte, Mitochondria, Mouse, mtDNA, Water buffalo (J. Reprod. Dev. 58: [323][324][325][326][327][328][329] 2012) S omatic cell nuclear transfer (SCNT) offers the possibility of preserving endangered species, and SCNT has been applied to many mammalian species [1]. However, owing to the limited availability of oocytes from wild animals, the cloning of endangered species benefits from the use of recipient oocytes from a related domestic species. However, this methodology results in the production of nuclear-cytoplasmic hybrids [2,3]. Despite numerous attempts in a wide variety of species, the number of live births of cloned offspring is still limited in instances that combine genetic compartments of closely related species [4,5]. The generation of animals through interspecies/intergeneric SCNT (iSCNT) poses several problems including mitochondrial/genomic DNA incompatibility, embryonic genome activation of the donor nucleus by the recipient oocyte, and availability of suitable foster mothers for iSCNT embryos. The species-specific nature of mitochondrial biogenesis and function makes this modeling particularly significant for iSCNT.F1 hybrids and iSCNT live offspring obtained using river buffalo (Bubal...

show abstract

Development of bovine–ovine interspecies cloned embryos and mitochondria segregation in blastomeres during preimplantation

Cited by 37 publications

References 38 publications

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Role of mitochondrial DNA replication during differentiation of reprogrammed stem cells

Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells

Contact Info

Product

Resources

About