Development of bovine serum albumin certified reference material

Wu, Liqing; Yang, Bin; Bi, Jiaming; Wang, Jing

doi:10.1007/s00216-011-4994-3

Cited by 28 publications

(16 citation statements)

References 10 publications

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“…Taylor and Williams (2010) and Young et al., (2012) and to our knowledge no replacement is receiving much consideration. The well characterised BSA standard (Wu et al., 2011) is thus still used extensively as a reference protein.…”

Section: Discussionmentioning

confidence: 99%

A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

Redmile-Gordon

Armenise²,

White³

et al. 2013

Soil Biology and Biochemistry

160

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Soil extracts usually contain large quantities of dissolved humified organic material, typically reflected by high polyphenolic content. Since polyphenols seriously confound quantification of extracted protein, minimising this interference is important to ensure measurements are representative. Although the Bradford colorimetric assay is used routinely in soil science for rapid quantification protein in soil-extracts, it has several limitations. We therefore investigated an alternative colorimetric technique based on the Lowry assay (frequently used to measure protein and humic substances as distinct pools in microbial biofilms). The accuracies of both the Bradford assay and a modified Lowry microplate method were compared in factorial combination. Protein was quantified in soil-extracts (extracted with citrate), including standard additions of model protein (BSA) and polyphenol (Sigma H1675-2). Using the Lowry microplate assay described, no interfering effects of citrate were detected even with concentrations up to 5 times greater than are typically used to extract soil protein. Moreover, the Bradford assay was found to be highly susceptible to two simultaneous and confounding artefacts: 1) the colour development due to added protein was greatly inhibited by polyphenol concentration, and 2) substantial colour development was caused directly by the polyphenol addition. In contrast, the Lowry method enabled distinction between colour development from protein and non-protein origin, providing a more accurate quantitative analysis. These results suggest that the modified-Lowry method is a more suitable measure of extract protein (defined by standard equivalents) because it is less confounded by the high polyphenolic content which is so typical of soil extracts.

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Section: Discussionmentioning

confidence: 99%

A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

Redmile-Gordon

Armenise²,

White³

et al. 2013

Soil Biology and Biochemistry

160

View full text Add to dashboard Cite

show abstract

“…The uncertainty of stability was calculated with t = 12 month using the following expression [23,24]: …”

Section: Uncertainty Of Homogeneitymentioning

confidence: 99%

“…The six sample groups were stored at 25°C for 0, 1, 2, 4, 6, and 12 months, respectively. The slope and standard deviation (SD) of the data points obtained in the stability tests were estimated using linear regression [23,24]. In the stability assessment, three replicate analyses were performed on the different samples.…”

Section: Stability Testmentioning

confidence: 99%

Development of a new certified reference material of high-purity chrysin for the quality control of traditional Chinese medicine

Yang

Gong

et al. 2016

Accred Qual Assur

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Chrysin, a naturally occurring flavone, extracted from traditional Chinese herbs and abundantly found in propolis has been known to exhibit a wide range of biological activities, such as antioxidant, anti-inflammatory, hypolipidemic, anti-hypertensive, anti-diabetic, and anti-tumour activities. Among this, its anticancer effects make chrysin promising to be a candidate drug. The objective of this study was to develop a new certified reference material (CRM) for the quality control of traditional Chinese medicine, chrysin, and the related pharmaceuticals. The studies of sample preparation, homogeneity, stability, value assignment, and uncertainty evaluation were accomplished in this paper. Two different methods, namely differential scanning calorimetry and coulometric titration, were utilized for the first time to determine the purity of chrysin. The purity of the highpurity chrysin CRM, after validation and evaluation, was certified to be (0.996 ± 0.015) g/g (k = 2).

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“…6 In addition, 17 kinds of proteinogenic amino acids are targeted for amino acid composition analysis or accurate quantification of protein/peptide. [7][8][9][10][11][12][13][14][15] In these methods, the constituent amino acids released from the original protein/ peptide through hydrolysis are quantified.…”

Section: Introductionmentioning

confidence: 99%

Development of High-purity Certified Reference Materials for 17 Proteinogenic Amino Acids by Traceable Titration Methods

et al. 2015

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Development of bovine serum albumin certified reference material

Cited by 28 publications

References 10 publications

A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

Development of a new certified reference material of high-purity chrysin for the quality control of traditional Chinese medicine

Development of High-purity Certified Reference Materials for 17 Proteinogenic Amino Acids by Traceable Titration Methods

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