Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds

Rosso, Leonardo Gómez; Shang, Chaoqun; Song, Qijian; Escamilla, Diana M.; Gillenwater, Jay H.; Zhang, Bo

doi:10.3390/ijms22052675

Cited by 20 publications

(14 citation statements)

References 46 publications

(45 reference statements)

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“…There is also interest in biofortification, especially iron to alleviate anemia problems (Bouis et al, 2011; Tan et al, 2017; Huertas et al, 2023). Variability is also available for these traits and QTLs associated with higher levels of protein, amino acids, oil, minerals or anti-nutritional compounds have been identified (Bohra et al, 2015; Shunmugam et al, 2015; Vaz Patto et al, 2015; Ma et al, 2017; Mendes et al, 2021; Rosso et al, 2021; Dhull et al, 2022; Tacke et al, 2022; Mecha et al, 2023; Zhou et al, 2023) that will allow selection processes to be accelerated. Breeding offers avenues to improve also sulfur amino acid composition and mineral biofortification (Krishnan et al, 2018; Jha et al, 2022; Joshi-Saha et al, 2022).…”

Section: Breeding Objectives To Better Meet Agroecological Requirementsmentioning

confidence: 99%

Plant breeding is needed to meet agroecological requirements: Legume crops as a case study

Rubiales

2023

Outlook Agric

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Legume-based cropping systems are regaining interest due to the environmental services and the variety of food and feed uses they provide, this having special interest in agroecological systems. There are many legume crops that can be adopted but focused breeding is needed to better meet the specific requirements of each system, especially in the situation of changing climate and often stressful environments. Standard breeding methods remain valid, from classical selection, to genomic assisted-breeding, profiting from the modern biotechnological and genomic approaches which are rapidly developing for most legume crops. Rather than focusing on the tools, emphasis is needed on the breeding targets, which might be different from those of high input agriculture such as improved response to symbiosis, nutrient and water use efficiency and tolerance to biotic and abiotic stresses becoming priorities. Breeding for these traits requires the infusion of genetic diversity from landraces or wild relatives by pre-breeding. Prospects and constraints are discussed.

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Section: Breeding Objectives To Better Meet Agroecological Requirementsmentioning

confidence: 99%

Plant breeding is needed to meet agroecological requirements: Legume crops as a case study

Rubiales

2023

Outlook Agric

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“…The three SSR markers, Satt409, Satt228, and Satt429, have shown tight linkage with this allele (M. S. Kim et al., 2006). More recently, kompetitive allele‐specific PCR (KASPar) markers have been developed in order to help breeders identify low‐TI soybean (Rosso et al., 2021).…”

Section: Other Functional Componentsmentioning

confidence: 99%

Soybean genetics, genomics, and breeding for improving nutritional value and reducing antinutritional traits in food and feed

Singer,

Lee,

Shea

et al. 2023

The Plant Genome

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Soybean [Glycine max (L.) Merr.] is a globally important crop due to its valuable seed composition, versatile feed, food, and industrial end‐uses, and consistent genetic gain. Successful genetic gain in soybean has led to widespread adaptation and increased value for producers, processors, and consumers. Specific focus on the nutritional quality of soybean seed composition for food and feed has further elucidated genetic knowledge and bolstered breeding progress. Seed components are historical and current targets for soybean breeders seeking to improve nutritional quality of soybean. This article reviews genetic and genomic foundations for improvement of nutritionally important traits, such as protein and amino acids, oil and fatty acids, carbohydrates, and specific food‐grade considerations; discusses the application of advanced breeding technology such as CRISPR/Cas9 in creating seed composition variations; and provides future directions and breeding recommendations regarding soybean seed composition traits.

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“…Besides, the linkage drags associated with KTI1 and KTI3 may introduce undesirable agronomic traits, which could be difficult to remove by backcrossing. Although Kompetitive Allele Specific PCR (KASP) markers associated with KTI3 and its mutant allele with 86% efficiency are available (Rosso, Shang et al 2021), attempts at developing molecular markers associated with KTI1 have not been successful (Gillman, Kim et al 2015). Therefore, it is highly desirable to develop new KTI1 mutant alleles that can be tagged with convenient molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Development of molecular markers of the Kunitz trypsin inhibitor mutant alleles generated by CRISPR/Cas9-mediated mutagenesis in soybean

Wang

Shea

Rosso

et al. 2022

Preprint

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The digestibility of soybean meal can be severely impacted by trypsin inhibitor (TI), one of the most abundant anti-nutritional factors present in soybean seeds. TI can restrain the function of trypsin, a critical enzyme that breaks down proteins in the digestive tract. Soybean accessions with low TI content have been identified. However, it is challenging to breed the low TI trait into elite cultivars due to a lack of molecular markers associated with low TI traits. We identified Kunitz trypsin inhibitor 1 (KTI1, Glyma01g095000) and KTI3 (Glyma08g341500) as two seed-specific TI genes. Mutant kti1 and kti3 alleles carrying small deletions or insertions within the gene open reading frames were created in the soybean cultivar Glycine max cv. Williams 82 (WM82) using the CRISPR/Cas9-mediated genome editing approach. The KTI content and TI activity both remarkably reduced in kti1/3 mutants compared to the WM82 seeds. There was no significant difference in terms of plant growth or maturity days of kti1/3 transgenic and WM82 plants in greenhouse condition. We further identified a T1 line, #5-26, that carried double homozygous kti1/3 mutant alleles, but not the Cas9 transgene. Based on the sequences of kti1/3 mutant alleles in #5-26, we developed markers to co-select for these mutant alleles by using a gel-electrophoresis-free method. The kti1/3 mutant soybean line and associated selection markers will assist in accelerating the introduction of low TI trait into elite soybean cultivars in the future.

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Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds

Cited by 20 publications

References 46 publications

Plant breeding is needed to meet agroecological requirements: Legume crops as a case study

Plant breeding is needed to meet agroecological requirements: Legume crops as a case study

Soybean genetics, genomics, and breeding for improving nutritional value and reducing antinutritional traits in food and feed

Development of molecular markers of the Kunitz trypsin inhibitor mutant alleles generated by CRISPR/Cas9-mediated mutagenesis in soybean

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