Development of CAPS markers to identify Indian tea (Camellia sinensis) clones with high catechin content

Elangbam, Meena; Misra, Arvind

doi:10.4238/gmr.15027860

Cited by 7 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CAPS is a combination of the RFLPs and PCR and it was originally named PCR-RFLPs, which using amplified DNA fragments digested with a restriction endonuclease to display restriction site polymorphisms (Singh, Hazarika, 2020). The technique involves amplification of a target DNA through PCR, followed by digesting with restriction enzymes (Elangbam, Misra, 2016). Therefore, CAPS markers rely on differences in restriction enzyme digestion patterns of PCR fragments caused by nucleotide polymorphism between samples.…”

Section: Cleaved Amplified Polymorphic Sequence (Caps)mentioning

confidence: 99%

Molecular markers for analysis of plant genetic diversity

Nam¹,

Hang²,

Linh³

et al. 2021

Vietnam J. Biotechnol.

View full text Add to dashboard Cite

Genetic diversity plays an important role in diversity conservation at multiple levels and supports to monitor and assess genetic variation. In plants, genetic diversity provides the ability to adapt and respond to environmental conditions that helps plants to survive through changing environments. Genetic diversity analyses based on molecular genetic markers are effective tools for conservation and reintroduction of rare and endangered species. In recent years, the development of various chemical and molecular techniques for studying genetic diversity has received great attention. While biochemical markers are primarily used in the diagnosis of pathogens, DNA markers have been developed and widely applied for identification of species and population based on the genotype of an organism that is more stable and not easily affected by the environmental factors. PCR-based molecular marker tools, such as restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSRs) are used for analysing the difference in the targeted DNA sequences. With the rapid and robust development of genomic sequencing technology it is now possible to obtain and analyse DNA sequences of the whole genome of studied organisms. However, each type of DNA markers has different principles, as well as the pros and cons of specificity. In this article, we review methods and point out DNA markers, which are considered as reliable and widely used tools for the detection of genetic variation. In addition, we present the application of DNA marker in analysing genetic diversity of wild, domestic and medicinal plants, as well as some perspectives on the future of DNA marker’s application in the analysis of genetic diversity.

show abstract

Section: Cleaved Amplified Polymorphic Sequence (Caps)mentioning

confidence: 99%

Molecular markers for analysis of plant genetic diversity

Nam¹,

Hang²,

Linh³

et al. 2021

Vietnam J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…These micro RNAs are short, non-coding RNA molecules, involved in post transcriptional mode of gene regulation and [ 16 ] thus effects on related phenotype [ 17 , 18 ]. There are a large number of molecular markers available for tea so far [ 19 ], however a very few are reported to be linked with some specific trait [ 20 ]. Therefore exploration and characterization of novel and already available markers are of prime point of interest.…”

Section: Introductionmentioning

confidence: 99%

Extrapolative microRNA precursor based SSR mining from tea EST database in respect to agronomic traits

et al. 2017

View full text Add to dashboard Cite

Tea (Camellia sinensis, (L.) Kuntze) is considered as most popular drink across the world and it is widely consumed beverage for its several health-benefit characteristics. These positive traits primarily rely on its regulatory networks of different metabolic pathways. Development of microsatellite markers from the conserved genomic regions are being worthwhile for reviewing the genetic diversity of closely related species or self-pollinated species. Although several SSR markers have been reported, in tea, the trait-specific Simple Sequence Repeat (SSR) markers, leading to be useful in marker assisted breeding technique, are yet to be identified. Micro RNAs are short, non-coding RNA molecules, involved in post transcriptional mode of gene regulation and thus effects on related phenotype. Present study deals with identification of the microsatellite motifs within the reported and predicted miRNA precursors that are effectively followed by designing of primers from SSR flanking regions in order to PCR validation. In addition to the earlier reports, two new miRNAs are predicting here from tea expressed tag sequence database. Furthermore, 18 SSR motifs are found to be in 13 of all 33 predicted miRNAs. Trinucleotide motifs are most abundant among all followed by dinucleotides. Since, miRNA based SSR markers are evidenced to have significant role on genetic fingerprinting study, these outcomes would pave the way in developing novel markers for tagging tea specific agronomic traits as well as substantiating non-conventional breeding program.

show abstract