Development of Capture Assays for Different Modifications of Human Low-Density Lipoprotein

Virella, Gabriel; Derrick, Michael; Pate, Virginia; Chassereau, Charlyne; Thorpe, Suzanne R.; Lopes‐Virella, Maria F.

doi:10.1128/cdli.12.1.68-75.2005

Cited by 32 publications

(43 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the data has suggested that modified LDL levels may correlate with progression of atherosclerosis and with the incidence of CVD, in general the results have been inconclusive. A major factor contributing to the limited number of reports on the significance of the levels of circulating forms of mLDL is the fact that over 90% of oxLDL-enriched and MDA-LDL-enriched LDL molecules circulate as IC [20]. Most methods proposed for their measurement in serum or plasma do not include steps designed to separate the antigens from the antibodies in order to measure the levels of mLDL present in circulation more accurately.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study

et al. 2012

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…The reactivity of mLDL separated from the IC with antibodies specific for oxLDL, MDA-LDL and AGE-LDL was then assayed by capture assays developed in our laboratory [20]. Coefficients of variation for 50 samples measured in two separate assays were 5.2% for oxLDL, 0.5% for MDA-LDL, and 8.3% for AGE-LDL.…”

Section: Methodsmentioning

confidence: 99%

The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study

et al. 2012

Self Cite

View full text Add to dashboard Cite

“…Briefly, 200 ml of 1 mg/ml AGE-BSA (Cambridge Biosciences, Cambridge, UK) in 0.05 m carbonate buffer (pH 9.6) was added in each well of a 96-well plate (Nunc; Maxisorb, Roskilde, Denmark), covered and incubated at 4 8C overnight. Next day, the plates were washed in PBS wash buffer (2 mM KH 2 PO 4 , 3 mM NaCl, 4.5 mM 2,4-hexadienoic acid potassium salt, 0.001% Tween-20), blocked (3% skimmed milk in ddH 2 O for 2 h), washed, samples and standards (triplicate wells of each) added, covered and incubated with 1:2000 dilution of 4G9, a previously validated (Virella et al 2005) goat anti-CML antibody (2 mg/ml; Meridian Life Sciences) with gentle agitation for 2 h. Standards of AGE-BSA in protein extraction buffer were serially diluted to give a range from 200 to 0.39 mg. Samples were diluted in a 0.05% Tween 20, 0.2% BSA and 75 nm PBS (pH 7.4) solution.…”

Section: Elisamentioning

confidence: 99%

Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism

Mallidis¹,

Czerwiec²,

Filippi³

et al. 2011

Reproduction

View full text Add to dashboard Cite

The synergistic effect of the co-morbidities that comprise metabolic syndrome (MetS) is increasingly being recognised as an important contributor in the pathology of a broad spectrum of seemingly disparate conditions. However, in terms of male reproductive function, beyond erectile dysfunction, little is known about the influence of this cohort (collectively or separately) on spermatogenesis and sperm quality. The aims of this study were to assess the reproductive tract of a MetS animal model for detrimental changes, to determine whether a group of compounds (advanced glycation end products and their receptor) known to cause cell dysfunction and DNA damage was present and assess whether hypogonadotropic hypogonadism was the main contributing factor for the changes seen. Animals fed a high-fat diet were found to have significantly increased cholesterol, triglycerides, blood glucose, mean arterial pressure and visceral fat levels. Although serum testosterone was decreased, no changes were seen in either testicular or epididymal histology. Immunolocalisation of N 3 -carboxymethyl-lysine and the receptor for advanced glycation end products was found in the testes, epididymides and sperm of the two treated groups of animals; however, ELISA did not show any difference in protein levels. Similarly, assessment of sperm nuclear DNA (nDNA) fragmentation by acridine orange test did not find significant differences in nDNA integrity. We conclude that the minimal effect on spermatogenesis and sperm quality seen in our model is probably due to the moderate increase of blood glucose rather than the hypogonadism.

show abstract

“…A determinação da LDL (-) pode ser realizada por cromatografia líquida (HPLC) de troca aniônica ou ELISA (enzyme linked immunosorbent assay) 29 . Os métodos cromatográficos são mais demorados e têm custo mais elevado em relação aos enzimaimunoensaios.…”

Section: Ldl Minimamente Oxidada Ou Ldl Eletronegativa (Ldl -)unclassified

Biomarcadores de peroxidação lipídica na aterosclerose

Abdalla

Sena

2008

Rev. Nutr.

View full text Add to dashboard Cite

A aterosclerose é caracterizada por uma resposta inflamatória crônica da parede arterial, iniciada por uma lesão do endotélio, cuja etiologia está relacionada à modificação oxidativa da lipoproteína de baixa densidade. O objetivo deste trabalho é apresentar os principais metabólitos envolvidos nos processos bioquímicos de peroxidação lipídica, discutindo as vantagens e desvantagens dos métodos empregados para a mensuração dos biomarcadores de peroxidação lipídica relacionados com a aterosclerose. A avaliação da oxidação das lipoproteínas pode ser realizada pela determinação dos produtos gerados durante a peroxidação lipídica, como os isoprostanos, hidroperóxidos lipídicos, aldeídos, fosfolípides oxidados e os produtos da oxidação do colesterol. A suscetibilidade das partículas de lipoproteína de baixa densidade à oxidação pode ser avaliada in vitro, após a indução da peroxidação lipídica por azoiniciadores radicalares lipossolúveis, hidrossolúveis, ou mais comumente, pelos íons cobre. Por outro lado, as modificações da lipoproteína de baixa densidade, pela ação das lipoxigenases e peroxidases, ou oxidação não-enzimática, resultam no aumento da carga negativa destas partículas e podem contribuir para a geração in vivo de uma subfração de lipoproteína de baixa densidade minimamente oxidada, denominada lipoproteína de baixa densidade eletronegativa (lipoproteína de baixa densidade). A determinação das concentrações desta partícula pode ser realizada em plasma por cromatografia líquida ou por imunoensaios..Diversos métodos podem ser utilizados para a avaliação dos biomarcadores de peroxidação lipídica in vivo e in vitro, porém, a definição do marcador mais adequado, depende de uma avaliação criteriosa das vantagens, desvantagens e particularidades de cada análise, levando-se em consideração os objetivos do estudo que será conduzido.

show abstract

Development of Capture Assays for Different Modifications of Human Low-Density Lipoprotein

Cited by 32 publications

References 31 publications

The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study

The levels of MDA-LDL in circulating immune complexes predict myocardial infarction in the VADT study

Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism

Biomarcadores de peroxidação lipídica na aterosclerose

Contact Info

Product

Resources

About