Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples

Botchkareva, Alexandra E.; Eremin, Sergei A.; Montoya, Ángel; Manclús, Juan J.; Mickova, Barbora; Rauch, P.; Fini, Fabiana; Girotti, Stefano

doi:10.1016/j.jim.2003.08.016

Cited by 56 publications

(42 citation statements)

References 19 publications

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“…Peroxidases (EC 1.11.1.7; donor:hydrogen-peroxidase oxidoreductase) are ubiquitous heme-containing enzymes of industrial interest for their widespread application in clinical chemistry (Krieg & Halbhuber 2003), environmental biosensor systems (Botchkareva et al 2003), bioconversion of industrial effluents (Song et al 2003) and synthesis of polymeric materials and aromatic chemicals (Shan et al 2003). Such applications are often carried out in systems containing organic solvents which greatly enhance solubilization of reactants and products, and improve yields, however, these systems often suffer from reduced activity, selectivity or stability of the enzyme, as compared to aqueous media (Klibanov 1997).…”

Section: Introductionmentioning

confidence: 99%

Thermal Stability and Activity Regain of Horseradish Peroxidase in Aqueous Mixtures of Imidazolium-Based Ionic Liquids

Machado

Saraiva

2005

Biotechnol Lett

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Thermal deactivation kinetics of horseradish peroxidase (HRP) were studied from 45 to 90 degrees C in phosphate buffer and 5-25% (v,w/v) 1-butyl-3-methylimidazolium tetrafluoroborate [BMIM][BF4] and 1-butyl-3-methylimidazolium chloride [BMIM][Cl]. HRP activity at 25 degrees C was not affected by the presence of ionic liquids up to 20% (v,w/v). Increasing the ionic liquids concentration up to 25% (v,w/v) changed the biphasic character of deactivation kinetics to an apparent single first-order step. The presence of 5-10% (v/v) [BMIM][BF4] significantly improved HRP thermal stability with lower activation energies for the deactivation second phase (83-87 kJ mol(-1)). After deactivation, enhanced activity regain of the enzyme, up to 70-80% of the initial activity, was found in 25% (v/v) [BMIM][BF4] and 10% (w/v) [BMIM][Cl] and correlated to prevalence of the deactivation first phase.

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Section: Introductionmentioning

confidence: 99%

Thermal Stability and Activity Regain of Horseradish Peroxidase in Aqueous Mixtures of Imidazolium-Based Ionic Liquids

Machado

Saraiva

2005

Biotechnol Lett

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“…Because surfactants (such as Tween-20) are commonly used in ELISA to reduce nonspecific interactions [18], their influence on assay performance (IC 50 , RLU max and RLU max /IC 50 ) was examined in the avidin-biotin CL-ELISA. Tween-20 concentrations higher than 0.1‰ reduced RLU max , whereas IC 50 increased with lower Tween-20 concentrations.…”

Section: Effect Of Tween-20mentioning

confidence: 99%

Comparison and development of two different solid phase chemiluminescence ELISA for the determination of albumin in urine

Zhao

Lin

2005

Analytica Chimica Acta

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Two chemiluminescence enzyme-linked immunosorbent assay (ELISA) methods based on avidin-biotin system and Fluorescein-isothiocyanate (FITC)-anti-FITC system as two different solid phase for the determination of albumin in urine were optimized, characterized and compared. Avidin or anti-FITC antibody was coated in the microplates to provide a universal solid phase which improved the variability and sensitivity. Alkaline phosphatase (ALP) labeled albumin and albumin from standard or patient samples compete for biotinylated-antibody or FITC labeled antibody binding sites. Enzyme activity in the bound fraction was detected with the chemiluminescence substrate 4-methoxy-4-(3-phosphatephenyl)-spiro-(1,2-dioxetane-3,2 -adamantane) (AMPPD). The influence of several physico-chemical parameters, such as incubation time, detergent concentration and solid phase conditions were also studied. For the two solid phase, both the linear range and the limit of detection of albumin were 0.15-15 and 0.089 g/mL, compared with the commercially ELISA kit, a good correlation were obtained. Moreover, three different solid phases include avidin-biotin system, FITC-anti-FITC system and the anti-albumin Ab directly coated the microtitre plates were compared mainly from the cost and precision, the results showed: (1) when the avidin-biotin system as solid phase, the amounts of the antibody used was only the 1/10 than those of the anti-FITC antibody as solid phase and antibody directly coated the microtiter plates; (2) the C.V. of these two solid-phase CL ELISA methods were lower than that of antibody directly coated solid phase.

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Section: Acetochlormentioning

confidence: 99%

“…The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel.…”

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confidence: 99%

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

et al. 2005

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Acetochlor[2-chloro-N -(ethoxymethyl)-N -(2-ethyl-6-methylphenyl)acetamide] belongs to chloroacetanilide herbicides, which have been widely introduced into the world agricultural practice [1][2][3]. Chloroacetanilide herbicides are relatively unstable in the environment and undergo degradation within six months; this is a valuable property of these herbicides. However, they can be detected in reservoirs, fish, and products of plant origin in amounts higher than maximum permissible levels as a result of their agricultural use. Acetochlor is a comparatively new pesticide; it was first registered in the United States in 1994. It has now replaced related compounds because of its better biodegradability and a relatively small carcinogenic effect. However, as well as the majority of pesticides, acetochlor is genotoxic, as was exemplified in plants, rats, and human lymphocytes [1]. Therefore, acetochlor residues should be monitored in natural samples such as water and soil.Chromatographic techniques, such as gas chromatography with atomic emission [4] or mass-spectrometric detection [5, 6], high-performance liquid chromatography with various detection techniques [7,8], and capillary electrophoresis [9] are commonly used for determining herbicides, in particular, acetochlor. These techniques are highly sensitive and accurate; however, they are also expensive and time-consuming. At the same time, it is necessary to perform simple and rapid screening in a great number of samples in order to detect herbicide-contaminated areas.Immunochemical techniques based on the specific binding of an analyte to corresponding specific antibodies are well suitable for this purpose. Enzyme immunoassay (EIA) has received the widest acceptance; the theory and practice of EIA was described in detail [10]. The use of EIA for the determination of pesticides has been reported in many reviews [11][12][13][14][15][16].EIA techniques are highly sensitive and simple; they can be performed under field conditions at the sampling site [17]. Since the 1990s, EIA has been used with increasing frequency for the screening of pesticides in natural and food samples. The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel. Therefore, the recent trend has been toward the development of nonseparation (homogeneous) immunoassay techniques, which significantly improve socalled high-throughput screening (HTS) [25]. Although an optimum immunoassay procedure for...

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Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples

Cited by 56 publications

References 19 publications

Thermal Stability and Activity Regain of Horseradish Peroxidase in Aqueous Mixtures of Imidazolium-Based Ionic Liquids

Thermal Stability and Activity Regain of Horseradish Peroxidase in Aqueous Mixtures of Imidazolium-Based Ionic Liquids

Comparison and development of two different solid phase chemiluminescence ELISA for the determination of albumin in urine

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

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