Acetochlor[2-chloro-N -(ethoxymethyl)-N -(2-ethyl-6-methylphenyl)acetamide] belongs to chloroacetanilide herbicides, which have been widely introduced into the world agricultural practice [1][2][3]. Chloroacetanilide herbicides are relatively unstable in the environment and undergo degradation within six months; this is a valuable property of these herbicides. However, they can be detected in reservoirs, fish, and products of plant origin in amounts higher than maximum permissible levels as a result of their agricultural use. Acetochlor is a comparatively new pesticide; it was first registered in the United States in 1994. It has now replaced related compounds because of its better biodegradability and a relatively small carcinogenic effect. However, as well as the majority of pesticides, acetochlor is genotoxic, as was exemplified in plants, rats, and human lymphocytes [1]. Therefore, acetochlor residues should be monitored in natural samples such as water and soil.Chromatographic techniques, such as gas chromatography with atomic emission [4] or mass-spectrometric detection [5, 6], high-performance liquid chromatography with various detection techniques [7,8], and capillary electrophoresis [9] are commonly used for determining herbicides, in particular, acetochlor. These techniques are highly sensitive and accurate; however, they are also expensive and time-consuming. At the same time, it is necessary to perform simple and rapid screening in a great number of samples in order to detect herbicide-contaminated areas.Immunochemical techniques based on the specific binding of an analyte to corresponding specific antibodies are well suitable for this purpose. Enzyme immunoassay (EIA) has received the widest acceptance; the theory and practice of EIA was described in detail [10]. The use of EIA for the determination of pesticides has been reported in many reviews [11][12][13][14][15][16].EIA techniques are highly sensitive and simple; they can be performed under field conditions at the sampling site [17]. Since the 1990s, EIA has been used with increasing frequency for the screening of pesticides in natural and food samples. The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel. Therefore, the recent trend has been toward the development of nonseparation (homogeneous) immunoassay techniques, which significantly improve socalled high-throughput screening (HTS) [25]. Although an optimum immunoassay procedure for...
Two fluorescence polarization immunoassays (FPIA) for detection of the insecticide DDT (DDT-specific FPIA) and the sum of DDT isomers and metabolites (DDT class-specific FPIA) were developed. Various fluorescein-labeled tracers were synthesized and studied in order to achieve a high sensitivity using different monoclonal antibodies. For DDTspecific assay the sensitivity, estimated as limit of detection, ANALYTICAL LETTERS was 12 ng/mL, with a linear working range between 20 and 1000 ng/mL. For DDT class-specific assay the sensitivity was 3 ng/mL, with a linear working range between 5 and 1000 ng/mL. Regarding specificity to other chlorinated pesticides, both immunoassays showed no cross-reaction (<0.1%) with 2,4-D, endosulfan, diuron and hexachlorocyclohexanes. Both FPIAs were successfully applied to the analysis of DDT in spiked tap water samples. The FPIAs for DDT are simple, rapid and homogeneous (without separation and washing steps). Total time of analysis is less than 1 min per sample. The developed FPIAs could be used for rapid screening of water samples for contamination with DDT and its isomers and metabolites.
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