Production of antibodies and development of enzyme-linked immunosorbent assays for the herbicide butachlor

Yakovleva, Julia; Zherdev, Anatoly V.; Popova, Valentina A.; Eremin, Sergei A.; Dzantiev, Boris B.

doi:10.1016/s0003-2670(03)00796-7

Cited by 32 publications

(17 citation statements)

References 35 publications

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“…Then the FP values were collected after incubation. A four-parameter logistic equation was used to fit the standard curves according to Equation (2) (Howe, Reichelt-Brushett, Clark, & Seery, 2017;Yakovleva, Zherdev, Popova, Eremin, & Dzantiev, 2003) …”

Section: Fpia Proceduresmentioning

confidence: 99%

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Liu

Wang

Zhou

et al. 2017

Food and Agricultural Immunology

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The herbicide propisochlor can be introduced into the water cycle via agricultural activities, thus possibly leading to harmful ecological effects. It is significant for the development of a rapid method for propisochlor analysis in water. In this work, a rapid homogeneous immunoassay based on fluorescence polarization immunoassay (FPIA) was firstly developed for the determination of propisochlor in pond water. The developed FPIA assay can be completed within only 14 min; the 50% inhibition concentration (IC 50 ) was 125.1 ng/mL and the limit of detection was 8.4 ng/mL. The recoveries of propisochlor in pond water ranged from 80.0% to 86.6%. A high performance liquid chromatography confirmation showed a good correlation with the developed FPIA (R 2 = 0.9748). The developed FPIA exhibited satisfactory rapidity, high sensitivity and excellent specificity, meeting the standards of the screening assay of propisochlor in pond water on site. The molecular modeling showed that the alkoxyalkyl of chloroacetamide herbicides exerted important effects on the herbicide-antibody binding.

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Section: Fpia Proceduresmentioning

confidence: 99%

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Liu

Wang

Zhou

et al. 2017

Food and Agricultural Immunology

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“…The limit of butachlor detection by this limit was 0.0002-0.114 lg/L. Butachlor recovery in the range of 70-188 % in a concentration of 10-100 ng/ml was observed when enzyme-linked immunosorbent assays were used for analysis of mineral, ground and surface water (Yakovleva et al 2003). For the assay, environmental water was spiked with butachlor at various concentrations and (Table 3) Phytoremediation Environmentally friendly, cost-effective, non-intrusive technique Phragmites australis, Zizania aquatica, and Acorus calamus (Yang et al 2011;Wang et al 2013) Nanoremediation Reduces cleanup time, certain contaminant concentration reduced to zero level, need for treatment and disposal of contaminated soil reduced Titanium dioxide nanoparticle (Mahmoodi et al 2007) was analyzed with ELISA which resulted in better butachlor recovery in all the tested samples.…”

Section: Residual Analysis From Watermentioning

confidence: 93%

“…A competitive enzyme-linked immunosorbent assay with photometric detection based on either immobilized antigen or antibody was developed for butachlor (Yakovleva et al 2003). A detection limit of 0.02 ng/ml was optimized in the study.…”

Section: Residual Analysis From Animalsmentioning

confidence: 99%

Addressing the environmental impacts of butachlor and the available remediation strategies: a systematic review

Abigail

Samuel

Ramalingam

2015

Int. J. Environ. Sci. Technol.

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Butachlor, a chloroacetanilide herbicide, used extensively all over the world as pre-emergence control of unwanted weeds. As a consequence, concerns about its potential adverse effects on the ecosystem and toxicity have risen. Several techniques have been used or are being investigated for effective removal of butachlor from the contaminated sites. This review reports the various toxicological studies conducted so far on the butachlor and the removal technologies available for its decontamination for better understanding. A new insight was also proposed after critical analysis of the merits and demerits of the removal technologies elucidated in the literature. An attempt was made to summarize the currently available analytical techniques reported for determination of butachlor in the environmental samples.

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“…Therefore, the assay principles and conditions developed for pesticides could be applied to the analysis of hazardous chemical agents. In the frame of our previous investigations [1][2][3][4] sets of reactants (antibodies, intermolecular conjugates) for specific immunodetection of the pesticides named above have been made. The reached detection limits of the pesticides Enzyme-Linked Immunosorbent Assays (ELISAs) are in the range from 0.02 to 5 ng/ml satisfying the needs of modern environmental monitoring and pollution control.…”

Section: Introductionmentioning

confidence: 99%

Immunochemical Approaches for Rapid Detection of Biologically Active Compounds

Dzantiev¹,

Zherdev²,

Byzova³

2005

Defense Against Bioterror

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Abstract:Express immunochemical techniques for determination of toxic compounds and pathogenic cells have been developed. Separation of reactants based on interaction between oppositely charged polyelectrolytes, namely polycation poly-N-ethyl-4-vinylpyridium and polyanion polymethacrylate, were used to reduce assay time. An extremely high rate and affinity of this interaction allowed formation of an immune complex in the solution followed by a quick separation of reactants. The polyelectrolyte-based assays were performed in both homogeneous and filtration formats. Total duration of the assay was 15-20 min, limit of pesticides detection -down to 0.2 ng/ml. The polyelectrolyte separation has been also used in electrochemical immunosensors. The assay protocol is based on the measurement of pH changes induced by a peroxidase label. A field-effect transistor is the sensitive element of the sensor, and specific immune complexes are formed at disposable porous membranes. An alternative approach is based on the application of screen-printed electrode with impregnated peroxidase. The set of reactants was used together with a portable amperometric device designed for express monitoring of hazardous compounds under out-of-lab conditions. The total assay time was 20 min, and the detection limit was 0.2 ng/ml. Immunochromatographic tests based on colloidal gold particles conjugated with antibodies have been developed for detection of low molecular weight antigens and bacterial cells. The method allows detection of bacterial cells including Mycobacterium tuberculosis and Legionella pneumophila during 10 min in concentrations down to 10 4 cell/mlImmunochemical methods are very effective for the detection of different biologically active compounds. High affinity of the antigen-antibody 1.

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Production of antibodies and development of enzyme-linked immunosorbent assays for the herbicide butachlor

Cited by 32 publications

References 35 publications

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Addressing the environmental impacts of butachlor and the available remediation strategies: a systematic review

Immunochemical Approaches for Rapid Detection of Biologically Active Compounds

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