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Yazynina, Elena V; Zherdev, Anatoly V.; Eremin, Sergei A.; Popova, Valentina A.; Dzantiev, B.G.

doi:10.1023/a:1013284101512

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…Peroxidase is commonly used as an enzymatic marker; alkaline phosphatase is used more rarely, and other enzymes such as β -galactosidase [35] and glucose oxidase [112,129] are used occasionally. Various techniques are used in ELISA for assessing enzyme activity: spectrophotometry is the most widespread technique; chemiluminescence [130] and fluorescence techniques [131,132], which are more sensitive, are also in use.…”

Section: Fundamentals Of Immunochemical Methods For Determining Pestimentioning

confidence: 99%

“…In Russia, early publications on ELISA for pesticides have been prepared in the group of Chkanikov and Umnov at the Institute of Phytopathology [142], and the first review on the immunochemical methods for determining pesticides and growth regulators was published by Zhemchuzhin and Gorobets in 1990 [143]. In Russia, the early publications in this area were devoted to the development of the ELISA determination of the following pesticides: 2,4-D [48,144]; 2,4,5-T [48]; simazine and atrazine [42]; and chlorsulfuron [112,142]. An extremely large number of procedures for determining various pesticides have been developed.…”

Section: Applications Of Elisamentioning

confidence: 99%

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Determination of pesticides by enzyme immunoassay

2005

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Section: Fundamentals Of Immunochemical Methods For Determining Pestimentioning

confidence: 99%

Section: Applications Of Elisamentioning

confidence: 99%

Determination of pesticides by enzyme immunoassay

2005

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Section: Acetochlormentioning

confidence: 99%

“…The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel.…”

mentioning

confidence: 99%

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

et al. 2005

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Acetochlor[2-chloro-N -(ethoxymethyl)-N -(2-ethyl-6-methylphenyl)acetamide] belongs to chloroacetanilide herbicides, which have been widely introduced into the world agricultural practice [1][2][3]. Chloroacetanilide herbicides are relatively unstable in the environment and undergo degradation within six months; this is a valuable property of these herbicides. However, they can be detected in reservoirs, fish, and products of plant origin in amounts higher than maximum permissible levels as a result of their agricultural use. Acetochlor is a comparatively new pesticide; it was first registered in the United States in 1994. It has now replaced related compounds because of its better biodegradability and a relatively small carcinogenic effect. However, as well as the majority of pesticides, acetochlor is genotoxic, as was exemplified in plants, rats, and human lymphocytes [1]. Therefore, acetochlor residues should be monitored in natural samples such as water and soil.Chromatographic techniques, such as gas chromatography with atomic emission [4] or mass-spectrometric detection [5, 6], high-performance liquid chromatography with various detection techniques [7,8], and capillary electrophoresis [9] are commonly used for determining herbicides, in particular, acetochlor. These techniques are highly sensitive and accurate; however, they are also expensive and time-consuming. At the same time, it is necessary to perform simple and rapid screening in a great number of samples in order to detect herbicide-contaminated areas.Immunochemical techniques based on the specific binding of an analyte to corresponding specific antibodies are well suitable for this purpose. Enzyme immunoassay (EIA) has received the widest acceptance; the theory and practice of EIA was described in detail [10]. The use of EIA for the determination of pesticides has been reported in many reviews [11][12][13][14][15][16].EIA techniques are highly sensitive and simple; they can be performed under field conditions at the sampling site [17]. Since the 1990s, EIA has been used with increasing frequency for the screening of pesticides in natural and food samples. The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel. Therefore, the recent trend has been toward the development of nonseparation (homogeneous) immunoassay techniques, which significantly improve socalled high-throughput screening (HTS) [25]. Although an optimum immunoassay procedure for...

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“…To date, most methods for SU analysis are based on liquid chromatography, 7 including liquid chromatography-mass spectrometry, [8][9][10][11] liquid chromatography-electrospray ionization-mass spectrometry, 1,6,12 liquid chromatography-diode array detector, 13 and high performance liquid chromatography (HPLC)-ultraviolet (UV). 14,15 Although less common, capillary electrophoresis, 16,17 immunoassays 18,19 and bioassays 20 have also been employed for the detection of SUs. Reasons for the greater popularity of HPLC methods include low detection limits, high accuracy and good reproducibility.…”

Section: Introductionmentioning

confidence: 99%