Development of chitosan-hyaluronic acid nanoparticles and study of their physico-chemical properties for targeted delivery of anticancer drugs

Abstract

Nanoparticles from chitosan and hyaluronic acid were obtained using ionotropic gelation technology. The size of the nanoparticles was investigated using electron microscopy and dynamic light scattering. Nanoparticles were obtained of an optimal size of ∼ 100 nm. A physical association method has been developed of encapsulating nanoparticles with doxorubicin, a well-known antitumor drug, and dinitrosyl iron complex (donor of nitric oxide). The surface potential of nanoparticles was determined by dynamic light scattering. It was shown that HA-DOX: CS {6: 1} nanoparticles were stable and had a potential of -45.6 meV. The localization of nanoparticles in the cancer cells has been studied by confocal and FLIM microscopy. It was found that nanoparticles with doxorubicin are located in the cell near and inside the nucleus. It was shown that the encapsulation of DNIC in the composition of nanoparticles significantly increases the stability of DNIC, prolongs the formation and increases the yield of nitric oxide. A unique nano-system has been created for the delivery of anticancer drugs into the cell.

Chitosan–Hyaluronic Acid Nanoparticles for Active Targeting in Cancer Therapy

“…Utilizing confocal and film imaging, Akentieva et al ( 2020) found out that doxorubicin nanoparticles were localized around cells and in the nucleus after using the ionic gelation method to synthesize the drug on a CHA-Np system. In addition, this method could increase the effectiveness of the active ingredient [84].…”

“…Therefore, this pH range is the only one where these two polymers can completely ionize and form strong electrostatic interactions [41]. Nanoparticle formulations for doxorubicin entrapment [84], paclitaxel entrapment [69], peptide entrapment [66], curcuminoids entrapment [101], and other nanoparticle formulations require ionic gelation processes.…”

Chitosan-Hyaluronan Nanoparticles for Vinblastine Sulfate Delivery: Characterization and Internalization Studies on K-562 Cells

“…These receptors are present in a great variety of cells, such as macrophages and lymphocytes, but are over-expressed in many types of cancer cells [ 19 ], hence representing a suitable marker for tumor targeting. Therefore, delivery systems that expose HY on the surface can increase intracellular drug accumulation, specifically in CD44 over-expressing cancer cells [ 20 , 21 , 22 ]; this can avoid serious side effects related to cancer therapy. Recently, Lallana et al [ 23 ] realized CS/HY NPs that efficiently deliver a large luciferase-encoding mRNA and a much smaller anti-Luc siRNA into cells overexpressing CD44 receptors.…”