Development of chloroplast‐specific microsatellite markers for molecular characterization of alloplasmic lines and phylogenetic analysis in wheat

Tomar, R. S.; Deshmukh, Rupesh; Naik, K. Bhojaraja; Tomar, S. M. S.; Vinod,

doi:10.1111/pbr.12116

Cited by 21 publications

(17 citation statements)

References 33 publications

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“…Understanding about genomic loci governing traits responsible for tolerance and availability of molecular markers tightly linked with it is a prerequisite for marker assisted selection ( Xu et al, 2012 ). A large amount of genomic data in the form of sequenced genomes and expression profiles are thus, impetuous for breeding for stress alleviation ( Sonah et al, 2011 ; Tomar et al, 2014 ). Use of genomics-based technologies has made a great impact in crop improvement programs.…”

Section: Genomicsmentioning

confidence: 99%

Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies

et al. 2017

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Abiotic stresses are the foremost limiting factors for agricultural productivity. Crop plants need to cope up adverse external pressure created by environmental and edaphic conditions with their intrinsic biological mechanisms, failing which their growth, development, and productivity suffer. Microorganisms, the most natural inhabitants of diverse environments exhibit enormous metabolic capabilities to mitigate abiotic stresses. Since microbial interactions with plants are an integral part of the living ecosystem, they are believed to be the natural partners that modulate local and systemic mechanisms in plants to offer defense under adverse external conditions. Plant-microbe interactions comprise complex mechanisms within the plant cellular system. Biochemical, molecular and physiological studies are paving the way in understanding the complex but integrated cellular processes. Under the continuous pressure of increasing climatic alterations, it now becomes more imperative to define and interpret plant-microbe relationships in terms of protection against abiotic stresses. At the same time, it also becomes essential to generate deeper insights into the stress-mitigating mechanisms in crop plants for their translation in higher productivity. Multi-omics approaches comprising genomics, transcriptomics, proteomics, metabolomics and phenomics integrate studies on the interaction of plants with microbes and their external environment and generate multi-layered information that can answer what is happening in real-time within the cells. Integration, analysis and decipherization of the big-data can lead to a massive outcome that has significant chance for implementation in the fields. This review summarizes abiotic stresses responses in plants in-terms of biochemical and molecular mechanisms followed by the microbe-mediated stress mitigation phenomenon. We describe the role of multi-omics approaches in generating multi-pronged information to provide a better understanding of plant–microbe interactions that modulate cellular mechanisms in plants under extreme external conditions and help to optimize abiotic stresses. Vigilant amalgamation of these high-throughput approaches supports a higher level of knowledge generation about root-level mechanisms involved in the alleviation of abiotic stresses in organisms.

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Section: Genomicsmentioning

confidence: 99%

Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies

et al. 2017

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“…Molecular markers have been applied to characterize cultivars independent of plant tissue or environmental effect and cultivar identification at very early stage of plant development [ 20 ]. Genetic diversity in the wheat has been examined using PCR-based molecular markers, such as Amplified Fragment Length Polymorphisms, AFLPs [ 21 ] and Sequence Tagged Microsatellite Sites (STMSs or, more generally, SSRs) [ 22 ] and cpSSRs [ 23 ]. The efficiency of polymorphism detection by SSR in wheat is high due to co-dominant nature, chromosome-specific, reproducibility and high information content [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Morpho-Agronomical Characterization of Root Architecture at Seedling and Reproductive Stages for Drought Tolerance in Wheat

et al. 2016

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Water availability is a major limiting factor for wheat (Triticum aestivum L.) production in rain-fed agricultural systems worldwide. Root architecture is important for water and nutrition acquisition for all crops, including wheat. A set of 158 diverse wheat genotypes of Australian (72) and Indian (86) origin were studied for morpho-agronomical traits in field under irrigated and drought stress conditions during 2010–11 and 2011-12.Out of these 31 Indian wheat genotypes comprising 28 hexaploid (Triticum aestivum L.) and 3 tetraploid (T. durum) were characterized for root traits at reproductive stage in polyvinyl chloride (PVC) pipes. Roots of drought tolerant genotypes grew upto137cm (C306) as compared to sensitive one of 63cm with a mean value of 94.8cm. Root architecture traits of four drought tolerant (C306, HW2004, HD2888 and NI5439) and drought sensitive (HD2877, HD2012, HD2851 and MACS2496) genotypes were also observed at 6 and 9 days old seedling stage. The genotypes did not show any significant variation for root traits except for longer coleoptiles and shoot and higher absorptive surface area in drought tolerant genotypes. The visible evaluation of root images using WinRhizo Tron root scanner of drought tolerant genotype HW2004 indicated compact root system with longer depth while drought sensitive genotype HD2877 exhibited higher horizontal root spread and less depth at reproductive stage. Thirty SSR markers were used to study genetic variation which ranged from 0.12 to 0.77 with an average value of 0.57. The genotypes were categorized into three subgroups as highly tolerant, sensitive, moderately sensitive and tolerant as intermediate group based on UPGMA cluster, STRUCTURE and principal coordinate analyses. The genotypic clustering was positively correlated to grouping based on root and morpho-agronomical traits. The genetic variability identified in current study demonstrated these traits can be used to improve drought tolerance and association mapping.

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“…The molecular markers development based on the latest wheat genome sequenced under specific abiotic stress conditions could serve identifying and screening genotypes based on salinity and tolerance to drought ( Tomar et al, 2014 ). Complete genome sequencing played a significant role in markers development and characterized the genes related to abiotic stress conditions in marker-assisted breeding ( Song et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Wheat omics: Classical breeding to new breeding technologies

Alotaibi

Alharbi

Alotaibi

et al. 2021

Saudi Journal of Biological Sciences

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Wheat is an important cereal crop, and its significance is more due to compete for dietary products in the world. Many constraints facing by the wheat crop due to environmental hazardous, biotic, abiotic stress and heavy matters factors, as a result, decrease the yield. Understanding the molecular mechanism related to these factors is significant to figure out genes regulate under specific conditions. Classical breeding using hybridization has been used to increase the yield but not prospered at the desired level. With the development of newly emerging technologies in biological sciences i.e., marker assisted breeding (MAB), QTLs mapping, mutation breeding, proteomics, metabolomics, next-generation sequencing (NGS), RNA_sequencing, transcriptomics, differential expression genes (DEGs), computational resources and genome editing techniques i.e. (CRISPR cas9; Cas13) advances in the field of omics. Application of new breeding technologies develops huge data; considerable development is needed in bioinformatics science to interpret the data. However, combined omics application to address physiological questions linked with genetics is still a challenge. Moreover, viroid discovery opens the new direction for research, economics, and target specification. Comparative genomics important to figure gene of interest processes are further discussed about considering the identification of genes, genomic loci, and biochemical pathways linked with stress resilience in wheat. Furthermore, this review extensively discussed the omics approaches and their effective use. Integrated plant omics technologies have been used viroid genomes associated with CRISPR and CRISPR-associated Cas13a proteins system used for engineering of viroid interference along with high-performance multidimensional phenotyping as a significant limiting factor for increasing stress resistance in wheat.

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Development of chloroplast‐specific microsatellite markers for molecular characterization of alloplasmic lines and phylogenetic analysis in wheat

Cited by 21 publications

References 33 publications

Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies

Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies

Molecular and Morpho-Agronomical Characterization of Root Architecture at Seedling and Reproductive Stages for Drought Tolerance in Wheat

Wheat omics: Classical breeding to new breeding technologies

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