Development of Collagen/Poly(vinyl alcohol)/Chondroitin Sulfate and Collagen/Poly(vinyl alcohol)/HA Electrospun Scaffolds for Tissue Engineering

Delgado-Rangel, Luis Humberto; Hernández-Vargas, Julia; Becerra-González, Marymar; Martı́nez-Torres, Ataúlfo; Prokhorov, E.; Campos, Janett Betzabe González

doi:10.1007/s12221-019-9341-x

Cited by 12 publications

(3 citation statements)

References 71 publications

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“…A number of previous studies have used PVA or PVP with combination of various polymers to fabricate scaffolds for various tissue engineering applications (Delgado‐Rangel et al, 2019; Mishra et al, 2019; Pourjavadi et al, 2020; Teixeira et al, 2020). However, the application of PVA–PVP has not been well explored for CTE.…”

Section: Introductionmentioning

confidence: 99%

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Pushp

Bhaskar

Kelkar

et al. 2021

Biotech & Bioengineering

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Polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) are the two most investigated biopolymers for various tissue engineering applications. However, their poor tensile strength renders them unsuitable for cardiac tissue engineering (CTE).In this study, we developed and evaluated PVA-PVP-based patches, plasticized with glycerol or propylene glycol (0.1%-0.4%; v:v), for their application in CTE. The cardiac patches were evaluated for their physico-chemical (weight, thickness, folding endurance, FT-IR, and swelling behavior) and mechanical properties. The optimized patches were characterized for their ability to support in vitro attachment, viability, proliferation, and beating behavior of neonatal mouse cardiomyocytes (CMs). In vivo evaluation of the cardiac patches was done under the subcutaneous skin pouch and heart of rat models. Results showed that the optimized molar ratio of PVA:PVP with plasticizers (0.3%; v-v) resulted in cardiac patches, which were dry at room temperature and had desirable folding endurance of at least 300, a tensile strength of 6-23 MPa and, percentage elongation at break of more than 250%.Upon contact with phosphate-buffered saline, these PVA-PVP patches formed hydrogel patches having the tensile strength of 1.3-3.0 MPa. The patches supported the attachment, viability, and proliferation of primary neonatal mouse CMs and were nonirritant and noncorrosive to cardiac cells. In vivo transplantation of cardiac patches into a subcutaneous pouch and on the heart of rat models revealed them to be biodegradable, biocompatible, and safe for use in CTE applications.

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Section: Introductionmentioning

confidence: 99%

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Pushp

Bhaskar

Kelkar

et al. 2021

Biotech & Bioengineering

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“…Increases in CS content led to the production of spherical micro- and nanoparticles turning the electrospinning process into electrospraying. Although increases in CS reduced nanofiber spinnability, the high surface-to-volume ratio present with the production of spherical micro- and nanoparticles could improve cellular adhesions [ 49 ].…”

Section: Biofabrication Techniquesmentioning

confidence: 99%

Sulfated Hydrogels in Intervertebral Disc and Cartilage Research

Lazarus

Bermudez-Lekerika

Farchione

et al. 2021

Cells

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Hydrogels are commonly used for the 3D culture of musculoskeletal cells. Sulfated hydrogels, which have seen a growing interest over the past years, provide a microenvironment that help maintain the phenotype of chondrocytes and chondrocyte-like cells and can be used for sustained delivery of growth factors and other drugs. Sulfated hydrogels are hence valuable tools to improve cartilage and intervertebral disc tissue engineering. To further advance the utilization of these hydrogels, we identify and summarize the current knowledge about different sulfated hydrogels, highlight their beneficial effects in cartilage and disc research, and review the biofabrication processes most suitable to secure best quality assurance through deposition fidelity, repeatability, and attainment of biocompatible morphologies.

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“…Polyvinyl alcohol (PVA), despite being a non-ionic hydrophilic synthetic polymer, has a lot of potential for electrospinning nanofibers. 3 PVA is not commonly used in technical applications but is frequently studied as a scaffold and wound dressing in biomedical applications, particularly tissue engineering. 4 The 5%–10% (w/v) of PVA solution has good fiber formation for single or bi-layer techniques.…”

Section: Introductionmentioning

confidence: 99%

Electrospun bio-nano hybrid scaffold from collagen, Nigella sativa, and chitosan for skin tissue engineering application

Alam

Shahid

Alimuzzaman

et al. 2023

Journal of Bioactive and Compatible Polymers

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The new sophisticated tissue engineering focused on producing nanocomposite with different morphologies for rapid tissue regeneration. In this case, utilizing nanotechnology with the incorporation of bio-based materials have achieved the interest of researchers. This research aims at developing hybrid bio-nano scaffold with collagen (Col), Nigella sativa ( Ns), and chitosan (Cs) by a bi-layered green electrospinning on polyvinyl chloride (PVA) layer in a different ratio for tissue regeneration. Field emission electron microscopy (FE-SEM), fourier transform infrared spectroscopy (FTIR), moisture management properties, tensile properties, antibacterial activity, and wound healing assessment of the fabricated hybrid bio-nano scaffolds were employed to investigate the different properties of hybrid bio-nano scaffolds. The results exhibit that the sample with Col (50%) and Ns (25%), Cs (25%) has good fiber formation with a mean diameter of 381 ± 22 nm. This bio-nano scaffold has a porosity of 78 ± 6.9% and a fast absorbing-slow drying nature for providing a moist environment. The antibacterial zones of inhibition (ZOI) against Staphylococcus aureus and Escherichia coli were 10 ± 1.3 and 8 ± 0.9 mm respectively, and appeared to be adequate to inhibit bacterial action. The wound healing assessment states that 84 ± 3.8% of wound closure occurs in just 10 days, which is quicker (1.5 times) than the duration of a commercial bandage. All of the findings suggest that the bio-nano scaffold could be useful for skin tissue engineering.

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Development of Collagen/Poly(vinyl alcohol)/Chondroitin Sulfate and Collagen/Poly(vinyl alcohol)/HA Electrospun Scaffolds for Tissue Engineering

Cited by 12 publications

References 71 publications

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Sulfated Hydrogels in Intervertebral Disc and Cartilage Research

Electrospun bio-nano hybrid scaffold from collagen, Nigella sativa, and chitosan for skin tissue engineering application

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