Development of colloidal gold-based immunochromatographic assay for rapid detection of Streptococcus suis serotype 2

Ju, Ying; Hao, Huaijie; Guo-hua, Xiong; Geng, Hongran; Zheng, Yinan; Wang, Jing; Cao, Yanghui; Yang, Yinhui; Cai, Xuehui; Jiang, Yongqiang

doi:10.1016/j.vetimm.2009.08.010

Cited by 38 publications

(16 citation statements)

References 14 publications

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“…For sensitivity, we have tested different concentrations of Leptospira culture, and the detection limit was 10 6 cells/ml. This result was almost the same as the detection limit of that achieved with the immunochromatographic assay for other bacterial species (25,37). For a specificity assay, we tested this single antibody system against several strains of Leptospira and bacteria that are commonly found in urine or are known to be the causative agents of urinary infection.…”

Section: Discussionmentioning

confidence: 53%

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Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Widiyanti¹,

Koizumi²,

Fukui³

et al. 2013

Clin. Vaccine Immunol.

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Leptospirosis is an infectious disease caused by the spirochete bacteria Leptospira spp. and is commonly found throughout the world. Diagnosis of leptospirosis performed by culture and microscopic agglutination tests is laborious and time-consuming. Therefore, we aimed to develop a novel immunochromatography (ICG)-based method for detecting Leptospira antigen in the urine of patients and animals. We used the 1H6 monoclonal antibody (MAb), which is specific to the lipopolysaccharide (LPS) that is common among Leptospira spp. The MAb was coupled to 40-nm-diameter colloidal gold, and the amounts of labeled antibody and immobilized antibody were 23 g and 2 g per test, respectively. Several strains of Leptospira and other bacterial species were used to evaluate the sensitivities and specificities of the assays we developed. The detection limit of the assays was 10 6 cells/ml when disrupted whole bacterial cells were used. The assays were Leptospira specific since they did not cross-react with non-Leptospira bacteria used in the study. Application of diagnostic assays was done on the urine samples of 46 Leptospira-infected hamsters, 44 patients with suspected leptospirosis, and 14 healthy individuals. Pretreatment of the urine samples by boiling and centrifugation (for ultrafiltration and concentration) eliminated nonspecific reactions that occurred in the assay. The sensitivity and specificity of the ICG-based lateral flow assay (LFA) were 89% and 87%, respectively, which were higher than those of the dipstick assay, which were 80% and 74%, respectively. In summary, this ICG-based LFA can be used as an alternative diagnostic assay for leptospirosis. Further development is still necessary to improve the assay.

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Section: Discussionmentioning

confidence: 53%

“…Polyclonal antibodies produced by the immunization of whole bacteria resulted in low specificity (37). By using only one kind of monoclonal antibody, we achieved better results than with a combination of polyclonal and monoclonal antibodies.…”

Section: Discussionmentioning

confidence: 88%

Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Widiyanti¹,

Koizumi²,

Fukui³

et al. 2013

Clin. Vaccine Immunol.

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“…Técnicas de isolamento de sorotipo específicas foram desenvolvidas, como a utilização de meios seletivos e diferenciais (Kataoka et al, 1991) e o isolamento imunomagnético (Gottschalk et al, 1999), além de outras técnicas sorológicas como imunofluorescência indireta (Robertson, 1985), Enzyme Linked ImmunonoSorbent Assay (ELISA) (Sepúlveda et al, 1996) e técnicas de imunocromatografia (Yang et al, 2007;Ju et al, 2010). No entanto, essas técnicas possuem sérias desvantagens, como variação nos resultados dependendo da concentração de anticorpos utilizada, baixa especificidade e incapacidade de diferenciar o sorotipo 2 do sorotipo 1/2 devido ao compartilhamento de antígenos comuns entre estes dois sorotipos (Elliot e Tai, 1978;Perch et al, 1983;Serhir et al, 1993;Sepúlveda et al, 1996;Gottschalk et al, 1999).…”

Section: Palavras-chaveunclassified

Prevalência de Streptococcus suis sorotipo 2: discussão da literatura brasileira

Soares

Paes

2013

Arq. Inst. Biol.

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Streptococcus suis é mundialmente considerado um dos patógenos de maior impacto sanitário e econômico na indústria suinícola. Dentre os sorotipos descritos como zoonóticos, o sorotipoÂ 2 é o mais frequentemente isolado de animais e humanos doentes na maioria dos países. O estudo da epidemiologia das infecções por S.Â suis no Brasil é importante para a implantação de medidas efetivas de controle. O objetivo do presente trabalho foi realizar uma revisão crítica da literatura brasileira, com suporte da literatura mundial, abordando o diagnóstico do agente e sua prevalência em animais clinicamente doentes e portadores sadios, com destaque para a prevalência do sorotipo 2 no país.

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“…Lateral flow immunochromatographic assay (LFIA) is an extended application of ELISA, it is rapid, simple, and appropriate for point-of-care test (Zhang et al, 2009;Dzantiev et al, 2014). The labels used in LFIA system mainly include colloidal gold nanoparticles (Kawatsu et al, 2006;Ju et al, 2010;Preechakasedkit et al, 2012;, magnetic nanoparticles Xu et al, 2009;Wang et al, 2015a), fluorescent microspheres (Xu et al, 2013), quantum dot and so on. Compared with other labels, magnetic nanoparticles can provide a higher sensitivity , because the magnetic signals from surface and internal membrane of LFIA can be detected by a Magnetic Assay Reader (MAR).…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive and flexible magnetic nanoprobe labeled immunochromatographic assay platform for pathogen Vibrio parahaemolyticus

Liu

Zhang

Wang

et al. 2015

International Journal of Food Microbiology

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Development of colloidal gold-based immunochromatographic assay for rapid detection of Streptococcus suis serotype 2

Cited by 38 publications

References 14 publications

Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Development of Immunochromatography-Based Methods for Detection of Leptospiral Lipopolysaccharide Antigen in Urine

Prevalência de Streptococcus suis sorotipo 2: discussão da literatura brasileira

A highly sensitive and flexible magnetic nanoprobe labeled immunochromatographic assay platform for pathogen Vibrio parahaemolyticus

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