Development of competitive enzyme-linked immunosorbent assays for boscalid determination in fruit juices

Abad‐Fuentes, Antonio; Esteve‐Turrillas, Francesc A.; Agulló, Consuelo; Abad‐Somovilla, Antonio; Mercader, Josep V.

doi:10.1016/j.foodchem.2012.04.090

Cited by 19 publications

(19 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PC standard curves were prepared in water, and they were mixed with the tracer dilution for direct competitive assays or with the polyclonal solution for indirect competitive assays in every of the prepared buffers. Changes in the PC inhibition curve parameters using distinct buffers were fitted by a multiple regression equation, including curvature and interaction terms, using Minitab 14.1 software (Minitab Inc., State College, PA, USA) as previously published [19].…”

Section: Buffer Studiesmentioning

confidence: 99%

“…Three buffer systems, i.e. citrate (pKa 2 = 4.8, pKa 3 = 6.4), phosphate (pKa 2 = 7.2), and Tris (pKa = 8.1), together with NaCl, were employed to adjust pH and ionic strength values, as described by Abad-Fuentes et al [19]. Tween 20 and FBS were added at a fixed concentration (0.05% and 10%, respectively) in order to reduce unspecific interactions.…”

Section: Buffer Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Exploring alternative hapten tethering sites for high-affinity anti-picoxystrobin antibody generation

Parra¹,

Esteve‐Turrillas²,

Abad‐Somovilla³

et al. 2011

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

The relevance of the linker tethering site in haptens was investigated for antibody generation and immunoassay development. Three derivatives of the strobilurin fungicide picoxystrobin were synthesized with the same functionalized spacer arm located at three different positions. Protein conjugates of those haptens were employed as immunogens and novel polyclonal antibodies were produced and characterized. All haptens afforded highly specific antibodies, but different affinities to the free analyte were observed among the obtained antisera. Then, competitive enzyme-linked immunosorbent assays were studied in several formats, and site heterology was confirmed as an effective strategy for detectability improvement. An indirect heterologous immunoassay was eventually selected and optimized, showing a limit of detection for picoxystrobin of 0.02 µg/L and a working range between 0.03 and 1.30 µg/L. Finally, the developed extraction and analytical procedures revealed a practical limit of quantification of 5 µg/kg for this fungicide in soybean sprouts, well below the maximum residue limits in the EU.

show abstract

Section: Buffer Studiesmentioning

confidence: 99%

Section: Buffer Conditionsmentioning

confidence: 99%

Exploring alternative hapten tethering sites for high-affinity anti-picoxystrobin antibody generation

Parra¹,

Esteve‐Turrillas²,

Abad‐Somovilla³

et al. 2011

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…To illustrate the synthetic potential of this one‐pot SSC method, 4′‐chlorobiphenyl‐2‐amine,49,50 known as the key intermediate in the synthesis of the fungicide Boscalid,51,52 was synthesized by using a one‐pot protocol. As illustrated in Scheme , the synthesis started from two readily available and cheap bromides, and in situ generated 4′‐chloro‐2‐nitrobiphenyl was then reduced readily to the amine in 72 % yield.…”

Section: Resultsmentioning

confidence: 99%

Efficient One‐Pot Cross‐Coupling of Two Aryl Halides by Stannylation/Stille Reaction in Water under Microwave Irradiation

et al. 2014

View full text Add to dashboard Cite

A simple and highly efficient one‐pot approach has been developed for the Pd(PPh3)4‐catalyzed cross‐coupling of two different aryl or heteroaryl bromides/iodides. This method involves the combined use of microwave irradiation and water as a single solvent to achieve sequential stannylation and Stille cross‐coupling reactions, which allows rapid access to a wide variety of biaryls in good to high yields. Furthermore, utilizing this step‐economical protocol, 2,5‐dibromopyridine was iteratively diarylated and the Boscalid intermediate was also synthesized in a one‐pot manner.

show abstract

“…Insecticides can be detected using UV-visible spectroscopy [9], enzyme-linked immunosorbent assay [10,11], Fourier Transform infrared spectroscopy [12], gas chromatography-mass spectroscopy [13–15], etc . However, these methods require expensive instrumentation, complicated pretreatment procedures and professional operators, which limits their application for real-time detection.…”

Section: Introductionmentioning

confidence: 99%

An Amperometric Immunosensor Based on Multi-Walled Carbon Nanotubes-Thionine-Chitosan Nanocomposite Film for Chlorpyrifos Detection

Sun

Cao

Gong

et al. 2012

Sensors

View full text Add to dashboard Cite

In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surface of MWCNTs-THI-CHIT/GCE using the crosslinking agent glutaraldehyde (GA). The modification procedure was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, a linear relationship between the relative change in peak current of different pulse voltammetry (DPV) and the logarithm of chlorpyrifos solution concentration was obtained in the range from 0.1 to 1.0 × 105 ng/mL with a detection limit of 0.046 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, making it a potential alternative tool for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.

show abstract

Development of competitive enzyme-linked immunosorbent assays for boscalid determination in fruit juices

Cited by 19 publications

References 21 publications

Exploring alternative hapten tethering sites for high-affinity anti-picoxystrobin antibody generation

Exploring alternative hapten tethering sites for high-affinity anti-picoxystrobin antibody generation

Efficient One‐Pot Cross‐Coupling of Two Aryl Halides by Stannylation/Stille Reaction in Water under Microwave Irradiation

An Amperometric Immunosensor Based on Multi-Walled Carbon Nanotubes-Thionine-Chitosan Nanocomposite Film for Chlorpyrifos Detection

Contact Info

Product

Resources

About