Glass-enamel coatings have been modified with nanosized particles, which expands the scope of their application and is a promising direction. It has been established that the introduction of nanosized zinc oxide in the amount of 0.5 wt. % into the composition of the enamel of the Na2O–B2O3–TiO2–SiO2 system imparts antibacterial properties to the coating against gram-positive and gram-negative bacteria. The meaning of the index of antibacterial activity of the surface of the enamel increases on average five times more in relation to Escherichia coli (E. coli) and six times more in relation to Staphylococcus aureus (St. aureus) compared with a control sample that does not contain zinc oxide.