Development of Core-Corona Type Polymeric Nanoparticles as an Anti-HIV-1 Vaccine

Wang, Xin; Akagi, Takami; Akashi, Mitsuru; Baba, Masanori

doi:10.2174/157019307779815857

Cited by 15 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously reported that antigen-carrying corecorona polystyrene NPs (PSNPs) were efficiently taken up by DCs and did enhance the immunogenicity of antigens (28,29). Intranasal immunization of mice with heat-inactivated HIV-1-capturing PSNPs demonstrated efficient production of HIV-1-specific neutralizing antibodies in the genital tract and CTL responses in the spleen (2,10).…”

mentioning

confidence: 99%

Induction of Potent CD8 ⁺ T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Wang

Uto

Akagi

et al. 2007

J Virol

Self Cite

View full text Add to dashboard Cite

The mainstream of recent anti-AIDS vaccines is a prime/boost approach with multiple doses of the target DNA of human immunodeficiency virus type 1 (HIV-1) and recombinant viral vectors. In this study, we have attempted to construct an efficient protein-based vaccine using biodegradable poly(␥-glutamic acid) (␥-PGA) nanoparticles (NPs), which are capable of inducing potent cellular immunity. A significant expansion of CD8 ؉ T cells specific to the major histocompatibility complex class I-restricted gp120 epitope was observed in mice intranasally immunized once with gp120-carrying NPs but not with gp120 alone or gp120 together with the B-subunit of cholera toxin. Both the gp120-encapsulating and -immobilizing forms of NPs could induce antigen-specific spleen CD8؉ T cells having a functional profile of cytotoxic T lymphocytes. Long-lived memory CD8؉ T cells could also be elicited. Although a substantial decay in the effector memory T cells was observed over time in the immunized mice, the central memory T cells remained relatively constant from day 30 to day 238 after immunization. Furthermore, the memory CD8 ؉ T cells rapidly expanded with boosting with the same immunogen. In addition, ␥-PGA NPs were found to be a much stronger inducer of antigen-specific CD8 ؉ T-cell responses than nonbiodegradable polystyrene NPs. Thus, ␥-PGA NPs carrying various HIV-1 antigens may have great potential as a novel priming and/or boosting tool in current vaccination regimens for the induction of cellular immune responses.

show abstract

mentioning

confidence: 99%

Induction of Potent CD8 ⁺ T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Wang

Uto

Akagi

et al. 2007

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example a new class of complex nano-structured hybrid microspheres with a raspberry-like structure can provide a unique micro/nano-environment which has found new applications in the material, chemical, medical and environmental sectors [16][17][18][19][20]. Bai et al have synthesized novel raspberry-like TiO 2 @yeast composites, which were effective photocatalysts for the degradation of dyes [21].…”

Section: Introductionmentioning

confidence: 99%

Biosorption of azo dyes by raspberry-like Fe3O4@yeast magnetic microspheres and their efficient regeneration using heterogeneous Fenton-like catalytic processes over an up-flow packed reactor

Song

Bai

Puma

et al. 2015

Reac Kinet Mech Cat

View full text Add to dashboard Cite

Biosorption of azo dyes by raspberry-like Fe3O4@yeast magnetic microspheres and their efficient regeneration using heterogeneous Fenton-like catalytic processes over an up-flow packed reactor his item ws sumitted to voughorough niversity9s snstitutionl epository y theGn uthorF Citation: yxqD F FFFet lFD PHISF fiosorption of zo dyes y rsperryE like peQyRdyest mgneti mirospheres nd their effiient regenertion using heterogeneous pentonElike tlyti proesses over n upEflow pked retorF etion uinetis wehnisms nd gtlysisD IIS@PAD ppF SRUESTPF Additional Information:• his pper ws epted for pulition in the journl etion uinetisD wehnisms nd gtlysis nd the definitive pulished version is ville t httpXGGdxFdoiForgGIHFIHHUGsIIIRREHISEHVSREz

show abstract

Poly(γ‐glutamic acid) nanoparticles as an efficient antigen delivery and adjuvant system: Potential for an AIDS vaccine

Wang

Uto

Akagi

et al. 2007

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

Antigen delivery systems using polymeric nanoparticles are of special interest as stable protein‐based antigen carriers. In the present study, novel biodegradable poly(γ‐glutamic acid) (γ‐PGA) nanoparticles were examined for their antigen delivery and immunostimulatory activities in vitro and in vivo. The uptake of ovalbumin by dendritic cells was markedly enhanced by γ‐PGA nanoparticles, and the ovalbumin was gradually released from γ‐PGA nanoparticles into the cells. In addition, γ‐PGA nanoparticles appeared to have great potential as an adjuvant, because they could induce the maturation of dendritic cells. Although not only ovalbumin‐encapsulating nanoparticles (OVA‐NPs) but also a simple mixture of ovalbumin and nanoparticles induced dendritic cell maturation, the only dendritic cells exposed to OVA‐NPs could strongly activate antigen‐specific interferon (IFN)‐γ‐producing T cells. Subcutaneous immunization of mice with human immunodeficiency virus type 1 (HIV‐1) p24‐encapsulating nanoparticles activated antigen‐specific IFN‐γ‐producing T cells in spleen cells and induced p24‐specific serum antibodies, as compared to immunization with p24 alone. Like ovalbumin, a mixture of p24 and nanoparticles also induced antigen‐specific serum antibodies but did not activate IFN‐γ‐producing T cells in spleen cells, suggesting that nanoparticles play a critical role in inducing cellular immune responses. Furthermore, γ‐PGA nanoparticles had a capacity comparable to that of the complete Freund's adjuvant (CFA) in inducing p24‐specific serum antibody. However, unlike CFA, they predominantly activated p24‐specific IFN‐γ‐producing T cells. Thus, γ‐PGA nanoparticles encapsulating various antigens may have great potential as novel and efficient protein‐based vaccines against infectious diseases, including HIV‐1 infection. J. Med. Virol. 80:11–19, 2008. © 2007 Wiley‐Liss, Inc.

show abstract

Development of Core-Corona Type Polymeric Nanoparticles as an Anti-HIV-1 Vaccine

Cited by 15 publications

References 20 publications

Induction of Potent CD8 ⁺ T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Induction of Potent CD8 ⁺ T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Biosorption of azo dyes by raspberry-like Fe3O4@yeast magnetic microspheres and their efficient regeneration using heterogeneous Fenton-like catalytic processes over an up-flow packed reactor

Poly(γ‐glutamic acid) nanoparticles as an efficient antigen delivery and adjuvant system: Potential for an AIDS vaccine

Contact Info

Product

Resources

About

Development of Core-Corona Type Polymeric Nanoparticles as an Anti-HIV-1 Vaccine

Cited by 15 publications

References 20 publications

Induction of Potent CD8 + T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Induction of Potent CD8 + T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Biosorption of azo dyes by raspberry-like Fe3O4@yeast magnetic microspheres and their efficient regeneration using heterogeneous Fenton-like catalytic processes over an up-flow packed reactor

Poly(γ‐glutamic acid) nanoparticles as an efficient antigen delivery and adjuvant system: Potential for an AIDS vaccine

Contact Info

Product

Resources

About

Induction of Potent CD8 ⁺ T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120

Induction of Potent CD8 ⁺ T-Cell Responses by Novel Biodegradable Nanoparticles Carrying Human Immunodeficiency Virus Type 1 gp120