Development of Economical Fertilizer-Based Media for Mass Culturing of Nannochloropsis oceanica

Abstract

This study was conducted to develop economical agricultural fertilizer media for the mass culturing of Nannochloropsis oceanica. Specific growth rates of N. oceanica cultured with differing concentrations of commercial compounds, urea fertilizers, and trace elements (Zn, Cu, Co, Mo) were compared with the growth rate in f/2 medium. Among the various added trace elements, CuSO 4 ‧5H 2 O was most effective for high growth of N. oceanica. The main nitrogen source in the agricultural fertilizers was ammonium, which was unsuitable for the growth of N. oceanica. Thus, the fertilizer at a lower concentration infused with NaNO 3 as a nitrogen source was more effective than fertilizer at higher concentrations. In this study, the growth of N. oceanica cultured with an agricultural fertilizer medium composed of compound fertilizer (41.7 mg/L), urea fertilizer (34.4 mg/L), NaNO 3 (150 mg/L), and CuSO4‧5H 2 O (0.0588 mg/L) was similar to that of N. oceanica cultured in f/2 medium.

Enhance Growth and Biochemical Composition of Nannochloropsis oceanica, Cultured under Nutrient Limitation, Using Commercial Agricultural Fertilizers

“…Our suggestions were in agreement with Guzman-Murillo et al who suggested that, CAGF media may be used to improve the biochemical composition of microalgae for the purposes of aquaculture, production of bioactive materials and biotechnology. Bae and Hur, (2011) found that the growth of N. oceanica cultured on fertilizer medium was similar to that of N. oceanica cultured in F/2 medium. On the other hand, our results disagree with Simental and Sanchez-Saavedra (2003) [22] who pointed that, comparing to F/2 medium, the using of liquid CAGF did not achieve any significant differences in cell concentration and growth rate of Navicula incerta, Nitzschia thermalis and Nitzschia laevis.…”

“…However, the effect of nonconventional media prepared from CAGF on microalgae growth [2,18,19] and biochemical composition [20,21] were investigated.…”

“…The high cost of microalgae production remains an obstacle for marine hatcheries [2]. Therefore, commercial agricultural fertilizers (CAGF) should be commonly used instead of F/2 culture medium [3].…”

Growth and Nutritional Composition of Eustigmatophyceae Monodus subterraneus and Nannochloropsis oceanica in Autotrophic and Mixotrophic Culture

“…The growth and nutritional composition of M. subterraneus and N. oceanica according to trophic culture method Culture of algae M. subterraneus (KMMCC-1348, 6.76 ± 0.61 µm (major axis), 3.66 ± 0.55 µm (minor axis)) and N. oceanica (KMMCC-359, diameter: 2.64 ± 0.41 µm), received from the Korea Marine Microalgae Culture Center (KMMCC), Pukyong National University, were cultured for this study. Jaworski's medium (JM) (Thompson et al 1988) for M. subterraneus and f/2 medium (Guillard and Ryther 1962) for N. oceanica were used, and the two species were cultured at 25 o C with continuous light of 80 µmol photons m −2 s −1 (Bae and Hur 2011a;Jo 2014).…”

Isolation of Trichoderma Spp. from Desert Soil, Biocontrol Potential Evaluation and Liquid Culture Production of Conidia Using Agricultural Fertilizers

“…The use of agricultural fertilizers as ingredients in culture medium for the growth of Trichoderma spp. is uncommon, but they have been used in microalgae scaling processes [17,18]. In Trichoderma, nitrogen fertilizers stimulate growth and conidia production and, in large amounts may have a synergistic effect on biocontrol effectiveness [19].…”