Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

Azim, Fazle; Rahman, Muhammad Mahbubur; Prodhan, Shamsul H.; Sikdar, Saif U; Zobayer, Nayem; Ashrafuzzaman, Md.

doi:10.3329/ijarit.v1i1-2.13935

Cited by 9 publications

(5 citation statements)

References 9 publications

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“…The explants were further sterilized with 0.1% HgCl 2 and Tween 20. Finally, the explants were dried on sterilized filter paper after washing several times with sterile distilled water [13]. The sterilized explants were inoculated in test tubes con-…”

Section: Methodsmentioning

confidence: 99%

In-Vitro Regeneration of Citrus sinensis (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Hasan¹,

Hasan²,

Foysal³

et al. 2019

AJPS

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In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% -50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA.These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.

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Section: Methodsmentioning

confidence: 99%

In-Vitro Regeneration of Citrus sinensis (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Hasan¹,

Hasan²,

Foysal³

et al. 2019

AJPS

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“…Removal of seed coat showed an early response for shoot formation. In case of sweet orange, highest (70%) shoot formation was obtained from seeds without seed coat (Azim et al, 2011). In this experiment direct regeneration from seed was done for obtaining 5 weeks old plantlets of kinnow and citron species.…”

Section: Direct Regeneration From Embryonic Seedmentioning

confidence: 99%

Establishment of an efficient callus induction method from leaf and stem in kinnow mandarin (Citrus reticulata Blanco.) and citron (Citrus medica L.)

Kamruzzaman¹,

Akther²,

Faruq³

et al. 2015

Afr. J. Biotechnol.

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Kinnow mandarin (Citrus reticulata Blanco.) is a highly adaptable variety among citrus cultivars in the climate of the region of Panjab, Pakistan and citron (Citrus medica L.) was the first of the citrus fruits known to become visible in the Mediterranean Basin. In Sylhet region of Bangladesh, it is commonly known as Zara lemon. The establishment of in vitro technique for seed germination and callus induction has been done for kinnow and citron in this experiment. In case of seed germination, ½ MS media supplemented with 6-benzylaminopurine (BAP) (0.5 mg/L)+ 1-naphthaleneacetic acid (NAA) (2.0 mg/L)+KIN (1.0 mg/L) shows best seed germination response (90%) for kinnow and BAP (1.0 mg/L)+NAA (0.5 mg/L) shows best (92%) for citron. For callus induction, 5 weeks old plantlets were used as a source of leaf and stem explants for both. For callus induction from leaf and stem of kinnow mandarin, 2,4-D (1.0 mg/L) shows best result (90%) for leaf while 2,4-dichlorophenoxyacetic acid (2,4-D) (1.0 mg/L)+BAP (0.5 mg/L)+NAA (0.25 mg/L) shows best callus response (95%) for stem explants. In case of citron, 2,4-D (1.0 mg/L) shows best callus response (80%) for both leaf and stem explants.

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“…In turmeric, 2.5-3.0 mg/L of 2,4-D was found to be optimum for callus induction and callus was initiated within two weeks of culture [45]. Similarly, Savita et al [46] reported maximum callus induction of 98.66% from leaf segments on MS medium supplemented with four mg/L 2,4-D in Citrus jambhiri and Citrus sinensis [47].…”

Section: Discussionmentioning

confidence: 99%

Studies on In vitro Surface Sterilisation and Antioxidants on Pomegranate (Punica granatum L.) cv. Bhagwa

S. G.,

Gunnaiah,

Patil

et al. 2024

PCBMB

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Pomegranate, regarded as the “Fruit of Paradise”, is one of the important fruit crops of tropical and subtropical regions. Pomegranate fruits are delicious and possess significant nutritional and medicinal benefits. Due to the increase in demand, the proliferation of pomegranate through tissue culture is essential to get high-quality planting material. However, microbial contamination in plant tissue culture is one of the bottlenecks for establishing aseptic cultures. Although multiple explant sterilisation methods have been developed for pomegranate, explant sterilisation methods can indeed vary from region to region due to the local environment and the mother plant. In the present study, 1-2 drops of tween 20 (20 min) + 500 mg/L carbendazim (30 min) + 500 mg/L streptomycin sulphate (10 min) + 100 mg/L citric acid (30 min) + 100 mg/L tebuconazole (folicur) (30 min) + 0.1% HgCl2 (1 min) + dipping explant after cutting the edges in sterile water (30 min) + dipping in 500 mg/L PVP (5 min) was found to be optimum in the prevention of microbial contamination with minimal cell death. Nodal segments-initiated callus at 54 days after culture, showing a callus induction percentage of 55.55% and a calli weight of 80 mg/explant.

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Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

Cited by 9 publications

References 9 publications

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Establishment of an efficient callus induction method from leaf and stem in kinnow mandarin (Citrus reticulata Blanco.) and citron (Citrus medica L.)

Studies on In vitro Surface Sterilisation and Antioxidants on Pomegranate (Punica granatum L.) cv. Bhagwa

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Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

Cited by 9 publications

References 9 publications

&lt;i&gt;In-Vitro&lt;/i&gt; Regeneration of &lt;i&gt;Citrus sinensis&lt;/i&gt; (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

&lt;i&gt;In-Vitro&lt;/i&gt; Regeneration of &lt;i&gt;Citrus sinensis&lt;/i&gt; (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Establishment of an efficient callus induction method from leaf and stem in kinnow mandarin (Citrus reticulata Blanco.) and citron (Citrus medica L.)

Studies on In vitro Surface Sterilisation and Antioxidants on Pomegranate (Punica granatum L.) cv. Bhagwa

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<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media