Development of endodontic sealers containing antimicrobial-loaded polymer particles with long-term antibacterial effects

Kitagawa, Haruaki; Kitagawa, Ranna; Tsuboi, Ririko; Hirose, Nanako; Thongthai, Pasiree; Sakai, Hirohiko; Ueda, Mayuka; Ono, Shunka; Sasaki, Junichi; Ooya, Tooru; Imazato, Satoshi

doi:10.1016/j.dental.2021.04.008

Cited by 10 publications

(12 citation statements)

References 41 publications

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“…Sealers can change their surrounding chemical environment (Baras et al, 2019; Kitagawa et al, 2021), inducing sustained alkalinity, which can also contribute to antimicrobial activity. Our pH analysis showed that only calcium silicate‐based sealers sustained high alkalinity over 28 days.…”

Section: Discussionmentioning

confidence: 99%

“…After chemo‐mechanical debridement, the root canal system is filled using gutta‐percha cones and root canal sealers (Kaur et al, 2015). Whilst filling materials can sequester residual microbes inside the canal and reduce their access to nutrients, the antimicrobial compounds in sealers can help limit growth and reduce bacterial burden (Kitagawa et al, 2021; Spangberg & Haapasalo, 2002). Thus, antimicrobial sealers could be an effective strategy to minimize the bacterial load in the root canal system.…”

Section: Introductionmentioning

confidence: 99%

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In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi‐species biofilm community

et al. 2022

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Aim: To evaluate the physicochemical properties of five root canal sealers and assess their effect on an ex vivo dental plaque-derived polymicrobial community. Methodology: Dental plaque-derived microbial communities were exposed to the sealers (AH Plus [AHP], GuttaFlow Bioseal [GFB], Endoseal MTA [ESM], Bio-C sealer [BCS] and BioRoot RCS [BRR]) for 3, 6 and 18 h. The sealers' effect on the biofilm biomass and metabolic activity was quantified using crystal violet (CV) staining and MTT assay, respectively. Biofilm community composition and morphology were assessed by denaturing gradient gel electrophoresis (DGGE), 16S rRNA sequencing and scanning electron microscopy. The ISO6876:2012 specifications were followed to determine the setting time, radiopacity, flowability and solubility. Obturated acrylic teeth were used to assess the sealers' effect on pH. Surface chemical characterization was performed using SEM with coupled energy-dispersive spectroscopy. Data normality was assessed using the Shapiro-Wilk test. One-way anova and Tukey's tests were used to analyze data from setting time, radiopacity, flowability and solubility. Two-way anova and Dunnett's tests were used for the data analysis from CV, MTT and pH. 16S rRNA sequencing data were analyzed for alpha (Shannon index and Chao analysis) and beta diversity (Bray-Curtis dissimilarities). Differences in community composition were evaluated by analysis of similarity (p < .05). Results: The sealers significantly influenced microbial community composition and morphology. All sealers complied with ISO6876:2012 requirements for setting time, radiopacity and flowability. Although only AHP effectively reduced the biofilm biomass, all sealers, except BRR, reduced biofilm metabolic activity. Conclusion: Despite adequate physical properties, none of the sealers tested prevented biofilm growth. Significant changes in community composition were observed. If observed in vivo, these changes could affect intracanal microbial survival, pathogenicity and treatment outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi‐species biofilm community

et al. 2022

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“…This feature of DAIs lays the basis for the development of antibacterial surfaces with long active durations. As shown by the representative reports on the development of “ long-term ” antibacterial surfaces ( Table 5 ) [ 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 ], various ingredients such as commercial antibiotics (tigecycline, vancomycin, amoxicillin, etc.) [ 121 , 132 ], metals or metal ions (silver, copper, or zinc) [ 124 , 125 , 129 ], and other drugs [ 127 , 128 ] were taken to equip implantable biomaterials (titanium, silicone, ceramics, etc.)…”

Section: Innovative Designs To Mitigate Device-associated Infectionsmentioning

confidence: 99%

“…As shown by the representative reports on the development of “ long-term ” antibacterial surfaces ( Table 5 ) [ 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 ], various ingredients such as commercial antibiotics (tigecycline, vancomycin, amoxicillin, etc.) [ 121 , 132 ], metals or metal ions (silver, copper, or zinc) [ 124 , 125 , 129 ], and other drugs [ 127 , 128 ] were taken to equip implantable biomaterials (titanium, silicone, ceramics, etc.) with prolonged antibacterial efficacy, ranging from days [ 127 , 131 , 133 ] to months [ 122 , 134 ].…”

Section: Innovative Designs To Mitigate Device-associated Infectionsmentioning

confidence: 99%

Antibacterial Designs for Implantable Medical Devices: Evolutions and Challenges

Cao

Qiao

Qin

2022

JFB

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The uses of implantable medical devices are safer and more common since sterilization methods and techniques were established a century ago; however, device-associated infections (DAIs) are still frequent and becoming a leading complication as the number of medical device implantations keeps increasing. This urges the world to develop instructive prevention and treatment strategies for DAIs, boosting the studies on the design of antibacterial surfaces. Every year, studies associated with DAIs yield thousands of publications, which here are categorized into four groups, i.e., antibacterial surfaces with long-term efficacy, cell-selective capability, tailored responsiveness, and immune-instructive actions. These innovations are promising in advancing the solution to DAIs; whereas most of these are normally quite preliminary “proof of concept” studies lacking exact clinical scopes. To help identify the flaws of our current antibacterial designs, clinical features of DAIs are highlighted. These include unpredictable onset, site-specific incidence, and possibly involving multiple and resistant pathogenic strains. The key point we delivered is antibacterial designs should meet the specific requirements of the primary functions defined by the “intended use” of an implantable medical device. This review intends to help comprehend the complex relationship between the device, pathogens, and the host, and figure out future directions for improving the quality of antibacterial designs and promoting clinical translations.

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“…Several studies have been conducted to develop antibacterial restorative materials. One of the effective approaches is to release the antibacterial components using a carrier, such as silver nanoparticles [ 1 , 2 ], ion-releasing glass fillers [ 3 , 4 , 5 ], and antimicrobial-loaded polymer particles [ 6 , 7 , 8 ]. However, these approaches typically employ a simple design to exhibit the release of antimicrobials under non-controlled conditions [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of pH-Responsive Zn2+-Releasing Glass Particles for Smart Antibacterial Restoratives

et al. 2022

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The on-demand release of antibacterial components due to pH variations caused by acidogenic/cariogenic bacteria is a possible design for smart antibacterial restorative materials. This study aimed to fabricate pH-responsive Zn2+-releasing glass particles and evaluate their solubilities, ion-releasing characteristics, and antibacterial properties in vitro. Three kinds of silicate-based glass particles containing different molar ratios of Zn (PG-1: 25.3; PG-2: 34.6; PG-3: 42.7 mol%) were fabricated. Each particle was immersed in a pH-adjusted medium, and the solubility and concentration of the released ions were determined. To evaluate the antibacterial effect, Streptococcus mutans was cultured in the pH-adjusted medium in the presence of each particle, and the bacterial number was counted. The solubility and concentration of Zn2+ released in the medium increased with a decrease in medium pH. PG-3 with a greater content of Zn demonstrated higher concentrations of released Zn2+ compared with PG-1 and PG-2. PG-2 exhibited bactericidal effects at pH 5.1, whereas PG-3 demonstrated bactericidal effects at pH values of 5.1 and 6.1, indicating that PG-3 was effective at inhibiting S. mutans even under slightly acidic conditions. The glass particle with 42.7 mol% Zn may be useful for developing smart antibacterial restoratives that contribute to the prevention of diseases such as caries on root surfaces with lower acid resistance.

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Development of endodontic sealers containing antimicrobial-loaded polymer particles with long-term antibacterial effects

Cited by 10 publications

References 41 publications

In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi‐species biofilm community

In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi‐species biofilm community

Antibacterial Designs for Implantable Medical Devices: Evolutions and Challenges

Fabrication of pH-Responsive Zn2+-Releasing Glass Particles for Smart Antibacterial Restoratives

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