Development of Fast HPLC Methods

The use of ultra high-performance liquid chromatography for studying hydrolysis kinetics of CL-20 and related energetic compounds

“…The use of ultra high-performance liquid chromatography (UHPLC) with columns packed with sub-2-m stationary phase allows for faster run times (3-5 times faster compared to conventional HPLC) [29][30][31]. This resulted in development of a chromatographic method with a run time of less than a minute, which is particularly important for CL-20 alkaline hydrolysis experiments in order to obtain data in real time scale.…”

“…Shorter analysis times allow for higher sample throughput and reduced solvent consumption and solvent removal costs, which can lead to significant cost savings down the line as a product moves to the production stage [29]. Reducing the column packing material particle diameter yields a lower plate height and allows for greater resolution per unit time for the overall separation [29].…”

“…Shorter analysis times allow for higher sample throughput and reduced solvent consumption and solvent removal costs, which can lead to significant cost savings down the line as a product moves to the production stage [29]. Reducing the column packing material particle diameter yields a lower plate height and allows for greater resolution per unit time for the overall separation [29]. The use of a UHPLC system (operates up to maximum 15,000 psi), and the implementation of "liophilic" mobile-phase additives for enhancement of the chromatographic selectivity for energetic compounds in a fast time scale is demonstrated.…”

“…The introduction of columns packed with sub-2-m stationaryphase particles in conjunction with ultra high pressure liquid chromatographic systems (UHPLC) is one of the latest contributions towards the trend of developing faster chromatographic methods in the analytical laboratory [29][30][31]. Shorter analysis times allow for higher sample throughput and reduced solvent consumption and solvent removal costs, which can lead to significant cost savings down the line as a product moves to the production stage [29].…”