Development of fast scanning X-ray fluorescence microscopy at the LNLS D09B-XRF beamline

Abstract

Today, the beamline comprises a new X-ray microfocusing optic based on a pair of bendable mirrors in the Kirkpatrick-Baez arrangement. The upgrading strategy also involved the migration of the control system to a NI PXI system (National Instruments Corporation), integrated with an open source EPICS/Linux platform. A new approach using a PXI system as a hardware triggering interface was developed in order to provide a continuous scanning mode of operation ("on-the-fly" scans) at D09B-XRF beamline. In this work, the main features of the LNLS XRF microprobe station as well as a description for setting up a general approach for fast scanning mode operation will be presented.

Fe3O4@SiO2 Nanoparticles Concurrently Coated with Chitosan and GdOF:Ce3+,Tb3+ Luminophore for Bioimaging: Toxicity Evaluation in the Zebrafish Model

“…The microfocused X-ray beam produced by a pair of curved mirrors allows to perform spatially resolved XRF measurements for elemental mapping of various elements and nanoparticles in biological specimen with very efficient sensitivity . The deep penetration capability and high spatial resolution make the SXRF one of the few techniques to localize the distribution of nanoparticles by mapping the chemical elements in thin histological sections of biological tissues, enabling a perspective to study in situ toxicity of nanoparticles.…”

“…X-ray fluorescence (XRF) imaging is considered a remarkable technique for chemical mapping of elements and nanoparticles, qualitatively and quantitatively in biological systems . A synchrotron light source generates a microfocused monochromatic X-ray beam that allows XRF microspectroscopic imaging with high spatial resolution and chemically mapping the various elements with high sensitivity at a precise histological level . This can facilitate our current understanding on the toxicity pathway of nanoparticles in biological system, studying in vivo the bionano interaction.…”

Towards on‐the‐fly X‐ray fluorescence mapping in the soft X‐ray regime

“…The potential of on‐the‐fly X‐ray fluorescence (XRF) has been already demonstrated in allowing fast elemental mapping on areas of the order of square millimeter or more, both with laboratory equipment and synchrotron setups . On‐the‐fly XRF relies on two fundamental requirements: (a) fast acquisition, meaning fast processing electronics with low dead times; and (b) good collection efficiency of the emitted XRF photons, meaning high incoming flux and/or good solid angle coverage.…”