Development of fibrinogen microspheres as a biodegradable carrier for tissue engineering

Rajangam, Thanavel; Paik, Hyun‐jong; An, Seong Soo A.

doi:10.1007/s13206-011-5211-3

Cited by 19 publications

(20 citation statements)

References 32 publications

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“…29 Furthermore, the excipients used showed key pharmaceutical properties, including biocompatibility, safety, 30 anti-infective properties, controlled release, 31,32 flexibility, emollient, adhesion, spreadability, and retention ability for film formulation. [33][34][35] The physicochemical properties, drug release, and cell compatibility characteristics of the scaffold film were studied. It was expected that this film has the potential to enable controlled drug delivery across the skin for prolonged time periods.…”

Section: Introductionmentioning

confidence: 99%

Dual drug-loaded nanoparticles on self-integrated scaffold for controlled delivery

Bennet¹,

Marimuthu²,

Kim³

et al. 2012

IJN

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Antioxidant (quercetin) and hypoglycemic (voglibose) drug-loaded poly-D,L-lactideco-glycolide nanoparticles were successfully synthesized using the solvent evaporation method. The dual drug-loaded nanoparticles were incorporated into a scaffold film using a solvent casting method, creating a controlled transdermal drug-delivery system. Key features of the film formulation were achieved utilizing several ratios of excipients, including polyvinyl alcohol, polyethylene glycol, hyaluronic acid, xylitol, and alginate. The scaffold film showed superior encapsulation capability and swelling properties, with various potential applications, eg, the treatment of diabetes-associated complications. Structural and light scattering characterization confirmed a spherical shape and a mean particle size distribution of 41.3 nm for nanoparticles in the scaffold film. Spectroscopy revealed a stable polymer structure before and after encapsulation. The thermoresponsive swelling properties of the film were evaluated according to temperature and pH. Scaffold films incorporating dual drug-loaded nanoparticles showed remarkably high thermoresponsivity, cell compatibility, and ex vivo drug-release behavior. In addition, the hybrid film formulation showed enhanced cell adhesion and proliferation. These dual drugloaded nanoparticles incorporated into a scaffold film may be promising for development into a transdermal drug-delivery system.

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Section: Introductionmentioning

confidence: 99%

Dual drug-loaded nanoparticles on self-integrated scaffold for controlled delivery

Bennet¹,

Marimuthu²,

Kim³

et al. 2012

IJN

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“…101,102 Fbg microspheres that were crosslinked with various concentrations of EDC (1, 3, 5 and 10 mM) revealed a slower rate of degradation with increasing EDC concentrations. 9 Compared with this, nanostructure aligned and highly porous Fbg microfibers that were crosslinked with EDC showed slower fibrinolytic degradation. Thus, increased Fbg cross-linking is not only based on the concentration of cross-linkers, but also depends on scaffold structural characteristics.…”

Section: Cross-linking Of Fbg and Fbn Micro/ Nanoscaffoldsmentioning

confidence: 88%

“…Using this method, the spheres size and uniformity can be controlled with the Fbg solution concentrations (wt%), flow rate, and the stirrer rotating speed. 9 Using the same method, Fbg nanospheres were also developed by further optimization of the above parameters, as shown in Figure 4. However, since the data is not yet published, the preparation method is not described here.…”

Section: Fbg and Fbn Microspheres/beads And Nanoparticles As Scaffoldsmentioning

confidence: 99%

“…3 Especially in tissue engineering, Fbg-and Fbn-based scaffolds induce ECM production that renders support to connective tissues, such as cartilage, ligament, bones, tendons, nerves, blood vessels, and skin. 4−8 Fbg and Fbn can be easily formulated into diverse structural scaffolds because of their micro-and nanocharacteristic; micro/nanoporous scaffolds, such as microspheres, 9 microfibers, 10 microtubes, 11 nanoparticles, 12 nanofibers, 13 and hydrogels, 14 have been formulated for diverse tissue engineering applications. In a vast range of severe pathological conditions, such as chronic wounds, diabetic ulcer, and ischemic heart disease, the rate of reepithelialization and neovascularization can predict the therapeutic approach towards perfusion and healing of damaged tissue.…”

Section: Introductionmentioning

confidence: 99%

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Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

Rajangam¹,

An²

2013

IJN

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Over the past two decades, many types of natural and synthetic polymer-based micro-and nanocarriers, with exciting properties and applications, have been developed for application in various types of tissue regeneration, including bone, cartilage, nerve, blood vessels, and skin. The development of suitable polymers scaffold designs to aid the repair of specific cell types have created diverse and important potentials in tissue restoration. Fibrinogen (Fbg)-and fibrin (Fbn)-based micro-and nanostructures can provide suitable natural matrix environments. Since these primary materials are abundantly available in blood as the main coagulation proteins, they can easily interact with damaged tissues and cells through native biochemical interactions. Fbg-and Fbn-based micro and nanostructures can also be consecutively furnished/ or encapsulated and specifically delivered, with multiple growth factors, proteins, and stem cells, in structures designed to aid in specific phases of the tissue regeneration process. The present review has been carried out to demonstrate the progress made with micro and nanoscaffold applications and features a number of applications of Fbg-and Fbn-based carriers in the field of biomaterials, including the delivery of drugs, active biomolecules, cells, and genes, that have been effectively used in tissue engineering and regenerative medicine.

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“…The amide III bond was assigned at 1245 cm -1 because of N-H bending and the complex vibration mode, as reported earlier for Fbg. 40,41 The peaks centered at 1450 and 1393 cm -1 were due to -CH 2 deformation and amino acid side-chain vibrations, respectively.…”

Section: Ft-ir Characterization Studymentioning

confidence: 99%

Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts

Rajangam¹,

An²

2013

IJN

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Abstract:The aim of this study was to fabricate fibrinogen (Fbg) microfibers with different structural characteristics for the development of 3-D tissue-engineering scaffolds. Fabricated Fbg microfibers were investigated for their biomolecule encapsulation, cell adhesion, and proliferations. Microfibers with three different concentrations of Fbg (5, 10, and 15 wt%) were prepared by a gel solvent-extraction method using a silicone rubber tube. Fbg microfibers were covalently modified with fibronectin (FN) by using water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the cross-linking agent. Fbg microfibers were characterized by their FN cross-linking properties, structural morphology, and in vitro degradation. Furthermore, FN/Fbg microfibers were evaluated for cell attachment and proliferation. The biocompatibility and cell proliferation of the microfibers were assessed by measuring adenosine triphosphate activity in C2C12 fibroblast cells. Cell attachment and proliferation on microfibers were further examined using fluorescence and scanning electron microscopic images. FN loading on the microfibers was confirmed by fluorescence and infrared spectroscopy. Surface morphology was characterized by scanning electron microscopy, and showed highly aligned nanostructures for fibers made with 15 wt% Fbg, a more porous structure for fibers made with 10 wt% Fbg, and a less porous structure for those made with 5 wt% Fbg. Controlled biodegradation of the fiber was observed for 8 weeks by using an in vitro proteolytic degradation assay. Fbg microfibers with highly aligned nanostructures (15 wt%) showed enhanced biomolecule encapsulation, as well as higher cell adhesion and proliferation than another two types of FN/Fbg fibers (5 and 10 wt%) and unmodified Fbg fibers. The promising results obtained from the present study reveal that optimal structure of Fbg microfibers could be used as a potential substratum for growth factors or drug release, especially in wound healing and vascular tissue engineering, in which fibers could be applied to promote and orient cell adhesion and proliferation.

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Development of fibrinogen microspheres as a biodegradable carrier for tissue engineering

Cited by 19 publications

References 32 publications

Dual drug-loaded nanoparticles on self-integrated scaffold for controlled delivery

Dual drug-loaded nanoparticles on self-integrated scaffold for controlled delivery

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts

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