Development of fluorescence/MR dual-modal manganese-nitrogen-doped carbon nanosheets as an efficient contrast agent for targeted ovarian carcinoma imaging

Abstract

Background Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients. Results In this study, manganese-nitrogen doped carbon nanosheets (Mn-N-CNSs) coupled with Anti-HE4 monoclonal antibody (Mn-N-CNSs@Anti-HE4) were synthesized for the specific and targeted fluorescence/MR dual-modal imaging of ovarian carcinoma. The prepared Mn-N-CNSs revealed excellent aqueous dispersity, good colloidal stability, great optical properties and high longtudinal relaxivity rate (r1 = 10.30 mM−1 s−1). Encouraged by the tunable photoluminiscence of the nanoprobe and Anti-HE4 targeting ligand, the ovarian carcinoma cells were specifically labeled by the Mn-N-CNSs@Anti-HE4 nanoprobe with multi-color fluorescences. Benefiting from the high r1 relaxivity, the nanoprobe exhibited targeted and enhanced MR contrast effect in the ovarian carcinoma cells and tumor bearing mice model. Besides, the high biocompatibility and easy excretion from the body of the nanoprobe were further confirmed in vivo. Conclusion The prepared Mn-N-CNSs@Anti-HE4 with excellent biocompatibility, high-performance and superior tumor-targeting ability provides a novel fluorescence/MR dual-modal nanoprobe for specific labeling and detection of ovarian carcinoma cells in vitro and in vivo.

Targeted OUM1/PTPRZ1 silencing and synergetic CDT/enhanced chemical therapy toward uveal melanoma based on a dual-modal imaging-guided manganese metal–organic framework nanoparticles

“…In the mildly acidic tumor microenvironment, the MOF in our nanodelivery system can be degraded, releasing Mn 2+ and loaded drugs including cisplatin and siRNAs. The released Mn 2+ not only facilitates CDT but will also affects the longitudinal relaxivity and MRI contrast effect, rendering the NPs a contrast agent for MRI [ 42 ]. As shown in Fig.…”

Single-Atom Manganese Anchored on Carbon Dots for Promoting Mitochondrial Targeting and Photodynamic Effect in Cancer Treatment

“…We notice that the CDs bound to Mn 2+ in a single atom might work simultaneously and accumulate in the mitochondria. This transport pathway may be blocked by the previously reported Mn-CDs due to encapsulation modification or coordination saturation. , In order to determine the PS’s intracellular position and the precise location of CDs within a cell, imaging colocalization was used. As shown in Figure a, the HepG2 cells were co-incubated with Mn/HA-CDs and HA-CDs for 2.5 h. Then, the mitochondria were stained with Mito tracker deep red (excitation at 644 nm and emission at 665 nm). , According to the images produced by laser confocal dual-channel excitation microscopy, the red fluorescence signals (from Mito tracker deep red) and the green fluorescence signals (from Mn/HA-CDs) overlap with equivalent fluorescence intensities.…”

“…This transport pathway may be blocked by the previously reported Mn-CDs due to encapsulation modification 25 or coordination saturation. 55,56 In order to determine the PS's intracellular position and the precise location of CDs within a cell, imaging colocalization was used. As shown in Figure 3a, the HepG2 cells were co-incubated with Mn/HA-CDs and HA-CDs for 2.5 h. Then, the mitochondria were stained with Mito tracker deep red (excitation at 644 nm and emission at 665 nm).…”

Tannin–Mn coordination polymer coated carbon quantum dots nanocomposite for fluorescence and magnetic resonance bimodal imaging

“…The poor sensitivity restricts the application of magnetic resonance imaging (MRI) for detecting small tumor nodules [1][2][3]. Many methods are used to improve the sensitivity of MRI contrast agents.…”