Development of fluorescent reporter tagged RIB gene cassettes for replicative transformation, early expression, and enhanced riboflavin production in Eremothecium ashbyi

Sengupta, Sudeshna; Kaufmann, Andreas M.; Chandra, T.S.

doi:10.1016/j.funbio.2012.07.008

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…In C. famata, overexpression of the RIB1 and RIB7 genes encoding GTP cyclohydrolase II and RF synthase led to a significant increase of the RF titer [30]. The RIB1 and RIB3 genes from A. gossypii, which respectively encode GTP-cyclohydrolase II (GCH II) and DHPB synthase were overexpressed in E. ashbyi to achieve RF overproduction [89].…”

Section: Overexpression Of the Rf Synthesis Pathwaymentioning

confidence: 99%

Production of riboflavin and related cofactors by biotechnological processes

Liu

Wang

et al. 2020

Microb Cell Fact

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Riboflavin (RF) and its active forms, the cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), have been extensively used in the food, feed and pharmaceutical industries. Modern commercial production of riboflavin is based on microbial fermentation, but the established genetically engineered production strains are facing new challenges due to safety concerns in the food and feed additives industry. High yields of flavin mononucleotide and flavin adenine dinucleotide have been obtained using whole-cell biocatalysis processes. However, the necessity of adding expensive precursors results in high production costs. Consequently, developing microbial cell factories that are capable of efficiently producing flavin nucleotides at low cost is an increasingly attractive approach. The biotechnological processes for the production of RF and its cognate cofactors are reviewed in this article.

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Section: Overexpression Of the Rf Synthesis Pathwaymentioning

confidence: 99%

Production of riboflavin and related cofactors by biotechnological processes

Liu

Wang

et al. 2020

Microb Cell Fact

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“…Among the most important vitamins for human consumption, riboflavin (vitamin B2) is primarily produced by two different microorganisms, Eremothecium ashbyi and Ashbya gossypii . To increase the yield of riboflavin, new and improved production processes have been developed using recombinant expression yeast models, such as Candida albicans (Sengupta et al, 2012 ). On the other hand, vitamin B12 is produced exclusively by bacteria and archaea, but not by fungi, plants, or animals.…”

Section: Therapeutic Drugs Of Microbial Originmentioning

confidence: 99%

What is the role of microbial biotechnology and genetic engineering in medicine?

Santos‐Beneit

2024

MicrobiologyOpen

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Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and therapeutic enzymes. Genetic engineering techniques, functional genomics, and synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene‐based technologies for medical applications.

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“…It seems to be necessary to increase the reaction rates of several enzyme activities. Interestingly, in the fungi Pichia pastoris and Eremothecium ashbyi, the overproduction of GTP cyclohydrolase II alone is sufficient to induce riboflavin overproduction [77,78]. In the study of P. pastoris by Marx et al [77], the largest riboflavin titer was obtained by overexpression of all six RIB genes, indicating that the regulation of riboflavin production involves several enzymes.…”

Section: Probabilistic Metabolic Control Analysismentioning

confidence: 99%

Thermodynamic and Probabilistic Metabolic Control Analysis of Riboflavin (Vitamin B2) Biosynthesis in Bacteria

Birkenmeier

Mack

Röder

2015

Appl Biochem Biotechnol

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In this study, we applied a coupled in silico thermodynamic and probabilistic metabolic control analysis methodology to investigate the control mechanisms of the commercially relevant riboflavin biosynthetic pathway in bacteria. Under the investigated steady-state conditions, we found that several enzyme reactions of the pathway operate far from thermodynamic equilibrium (transformed Gibbs energies of reaction below about -17 kJ mol(-1)). Using the obtained thermodynamic information and applying enzyme elasticity sampling, we calculated the distributions of the scaled concentration control coefficients (CCCs) and scaled flux control coefficients (FCCs). From the statistical analysis of the calculated distributions, we inferred that the control over the riboflavin producing flux is shared among several enzyme activities and mostly resides in the initial reactions of the pathway. More precisely, the guanosine triphosphate (GTP) cyclohydrolase II activity, and therefore the bifunctional RibA protein of Bacillus subtilis because it catalyzes this activity, appears to mainly control the riboflavin producing flux (mean FCCs = 0.45 and 0.55, respectively). The GTP cyclohydrolase II activity and RibA also exert a high positive control over the riboflavin concentration (mean CCCs = 2.43 and 2.91, respectively). This prediction is consistent with previous findings for microbial riboflavin overproducing strains.

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Development of fluorescent reporter tagged RIB gene cassettes for replicative transformation, early expression, and enhanced riboflavin production in Eremothecium ashbyi

Cited by 9 publications

References 30 publications

Production of riboflavin and related cofactors by biotechnological processes

Production of riboflavin and related cofactors by biotechnological processes

What is the role of microbial biotechnology and genetic engineering in medicine?

Thermodynamic and Probabilistic Metabolic Control Analysis of Riboflavin (Vitamin B2) Biosynthesis in Bacteria

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