In the global in vitro diagnostic(IVD) market, point-of-care testing(POCT) features the largest market share, while POCT in China features small market share. With the development of consumption upgrading, urbanization and medical reform of China, this kind of real-time detection without central laboratory features huge market demand and potential. POCT is a testing method, which can obtain test results in just few minutes with portable equipment and supporting reagents. In this paper, a minitype modularized fluorescence microscopic imaging system is proposed, which is used for blood cells detection, microbial observation and pathological section scanning. In order to meet the needs of portable, miniaturized and chip-type, a linear objective lens switching and focusing mechanism (LOLSFM) has been designed and developed, which changed the rotating disk switching mode of existing microscope. Finally, the precision and verticality of the LOLSFM are verified by experiments. The imaging quality of minitype modularized fluorescence microscopic imaging system.