Development of gelatin flakes, a new type of anti-adhesive material: a preliminary study of in vivo rat adhesion models

Tsujimoto, Hiroyuki; Takamori, Hideki; Tsuji, Misaki; Hayashi, Maho; Ikeda, Junki; Orikasa, Taichi; Torii, Hiroko; Ozamoto, Yuki; Suzuki, Shuko; Morita, Shinichiro; Ikada, Yoshito; Hagiwara, Akeo

doi:10.1007/s00595-013-0608-3

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…This study showed severe adhesions in the control group in which the fibrin glue sealing sheet was applied. In contrast, anti-adhesion effect of gelatin has been reported elsewhere [25,[27][28][29][30][31]. A thermally treated gelatin sheet, reinforced with bioabsorbable poly(glycolic acid) mesh, exhibited reduced formation of pleural and pericardial adhesion and inflammatory reaction in a canine model [29,30].…”

Section: Postoperative Adhesionsmentioning

confidence: 86%

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Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Yamashita

Tabayashi

et al. 2015

Bio-Medical Materials and Engineering

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BACKGROUND: The bilayer gelatin sealing sheet was developed as a safe, effective, easy-to-handle and low-cost hemostatic agent. OBJECTIVE: To examine the feasibility of gelatin sealing sheets using a canine arterial hemorrhage model. METHODS:In vivo degradation of gelatin sealing sheets was examined by implanting subcutaneously in rats. For the hemostatic and anti-adhesion efficacy investigations, femoral arteries of dogs were pricked with syringe needle to make a small hole and a gelatin (i.e. experimental group) or fibrin glue sealing sheet (i.e. control group) was applied on the hole to stop bleeding (n = 8). After discontinuation of the bleeding, the skin incisions were closed and re-examined 4 weeks postoperatively. RESULTS: From the degradation study, 4 h thermally treated gelatin sheet which degraded within 3 weeks in vivo was chosen for the further hemostatic study. In all cases of gelatin and fibrin glue sealing sheets, bleeding from the needle hole on canine femoral arteries was effectively stopped. Postoperative adhesions and inflammation at the site in the experimental group were significantly less than those in the control group (P < 0.01 for adhesion scores). CONCLUSIONS: The gelatin sealing sheet was found to be as effective as the fibrin glue sealing sheet as a surgical hemostatic agent, and more effective in preventing postoperative adhesions.

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Section: Postoperative Adhesionsmentioning

confidence: 86%

“…It has been reported that unstable glutaraldehyde polymer released from the glutaraldehyde crosslinked material caused cytotoxicity [26]. Alternatively, gelatin can be crosslinked by UV irradiation [27] or thermal treatment [28][29][30][31]. These techniques do not require chemical agents, and hence, are safer options.…”

Section: Surgical Hemostatic Agentsmentioning

confidence: 99%

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Yamashita

Tabayashi

et al. 2015

Bio-Medical Materials and Engineering

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“…Currently, medical absorbable anti-adhesion films [ 14 ] are most widely used both domestically and internationally, such as Seprafilm [ 15–17 ], Interceed [ 18 ] etc. Solid anti-adhesive film [ 19 ] has a physical barrier effect that can isolate inflammatory cells and inflammatory factors, and the effect is stable and long-lasting. However, it is challenging to cover irregular or large areas of injury, and the surgical exposure area is too large, so it is suitable for open surgery but not for minimally invasive surgery.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effectiveness of alginate-based hydrogels in preventing peritoneal adhesions

Meng

Wang

Liu

et al. 2023

Regenerative Biomaterials

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Infertility and intestinal blockage are just two examples of the postoperative consequences that can arise from peritoneal damage, which can also result in severe peritoneal fibrosis and peritoneal adhesions. Peritoneal adhesions are still not effectively treated, and both pharmaceutical therapy and biomaterial barriers have only had modest preventative effects. In this work, we looked into the effectiveness of in-place injectable sodium alginate hydrogel for peritoneal adhesion prevention. The findings demonstrated that sodium alginate hydrogel promoted human peritoneal mesothelial cell proliferation and migration, prevented peritoneal fibrosis by suppressing the production of transforming growth factor-β1, and, most importantly, promoted mesothelium self-repair. These findings imply that this brand-new sodium alginate hydrogel is a good candidate material for peritoneal adhesion prevention.

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“…4,5 The solution of this problem could be in the preparation of anti-adhesion materials based on biopolymers. 6,7 Among the numerous natural polymers, it is important to highlight pectin and chitosan as biodegradable, biocompatible, non-toxic, and bioadhesive polysaccharides, which can potentially be used to create new biomaterials. [8][9][10][11] Chitosan -a cationic polysaccharide, the deacetylated derivative of chitin, a natural polysaccharide -is the main component of the integument of arthropods and some fungi.…”

Section: Introductionmentioning

confidence: 99%

Prevention of postoperative adhesions by biodegradable cryogels from pectin and chitosan polysaccharides

Konovalova

Markov

Popova

et al. 2017

Journal of Bioactive and Compatible Polymers

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The main complication that may arise after surgery is the formation of adhesions. The current trend in the prevention of postoperative adhesions is the application of anti-adhesive barrier materials for the separation of wound tissue during the critical period of mesothelial repair and healing. In this work, cryogels based on pectin and chitosan were obtained by the ionic cryotropic gelation method. It was found that the Heracleum L. pectin cryogels are more elastic (73 ± 6 kPa) than the apple pectin cryogels (29 ± 11 kPa). The addition of chitosan with different physical and chemical characteristics did not significantly affect the elasticity of pectin–chitosan cryogels. The greatest swelling ability was achieved during in vivo incubation of Heracleum L. pectin cryogels and Heracleum L. pectin with reacetylated chitosan cryogels (17.1 ± 1.6 and 14.2 ± 2.0 g/g, respectively). It was found that the complete biodegradation of apple pectin cryogels occurred within 24 h of incubation in the rat abdominal cavity. Heracleum L. pectin cryogels were encapsulated in a fibrous capsule and detected in the abdominal cavity after 168 h. Maximum anti-adhesion effect was observed through the use of apple pectin cryogels (0 ± 0.5 score). Significant anti-adhesive effect was also observed through the use of apple pectin–reacetylated chitosan cryogels (1 ± 0.5 score). Due to the high anti-adhesive activity, such cryogels can be recommended for the development of a new barrier material for use in surgery. The potential anti-adhesive mechanism of apple pectin cryogels which may be attributed to a combination of barrier function and bioactivity of cryogels components was discussed.

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Development of gelatin flakes, a new type of anti-adhesive material: a preliminary study of in vivo rat adhesion models

Cited by 6 publications

References 12 publications

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Evaluation of the effectiveness of alginate-based hydrogels in preventing peritoneal adhesions

Prevention of postoperative adhesions by biodegradable cryogels from pectin and chitosan polysaccharides

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