Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing

Kim, Young-Cheon; Ahn, Woo Seok; Cha, Ahra; Jie, Eun Yee; Kim, Suk Weon; Hwang, Byung-Ho; Lee, Sanghyeob

doi:10.1007/s11816-021-00732-y

Cited by 14 publications

(4 citation statements)

References 40 publications

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“…Therefore, the PEG-Ca 2+ -mediated transformation of plant protoplasts has great advantages and application value in gene function analysis and breeding design [ 86 ]. However, there are still some problems, such as difficulty in the protoplast separation of special tissues and organs, as well as the low frequency of callus regeneration [ 157 ].…”

Section: Research and Application Of Genetic Transformation Technolog...mentioning

confidence: 99%

Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli

Zhang,

Meng,

Liu

et al. 2024

Genes

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Broccoli, a popular international Brassica oleracea crop, is an important export vegetable in China. Broccoli is not only rich in protein, vitamins, and minerals but also has anticancer and antiviral activities. Recently, an Agrobacterium-mediated transformation system has been established and optimized in broccoli, and transgenic transformation and CRISPR-Cas9 gene editing techniques have been applied to improve broccoli quality, postharvest shelf life, glucoraphanin accumulation, and disease and stress resistance, among other factors. The construction and application of genetic transformation technology systems have led to rapid development in broccoli worldwide, which is also good for functional gene identification of some potential traits in broccoli. This review comprehensively summarizes the progress in transgenic technology and CRISPR-Cas9 gene editing for broccoli over the past four decades. Moreover, it explores the potential for future integration of digital and smart technologies into genetic transformation processes, thus demonstrating the promise of even more sophisticated and targeted crop improvements. As the field continues to evolve, these innovations are expected to play a pivotal role in the sustainable production of broccoli and the enhancement of its nutritional and health benefits.

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Section: Research and Application Of Genetic Transformation Technolog...mentioning

confidence: 99%

Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli

Zhang,

Meng,

Liu

et al. 2024

Genes

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“…In broccoli, a QTL mapping study indicates that two QTLs named Pur7.1 and Pur9.1 facilitate the biosynthesis of anthocyanin, and induce the purple cauliflower phenotype ( Liu et al., 2022 ). Further, the CRISPR mutation of BolMYB28 increases glucoraphanin content in the leaves ( Kim et al., 2022 ), which induces a healthcare usage of broccoli. For rapeseed, the edible value is reflected through bolting, and the ornamental value is reflected through flowering.…”

Section: Pre-breeding For Other Types Of Terrace Vegetablesmentioning

confidence: 99%

Mainstreaming production and nutrient resilience of vegetable crops in megacities: pre-breeding for terrace cultivation

Ma,

Yuan,

Fang

2023

Front. Plant Sci.

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Modern megacities offer convenient lifestyles to their citizens. However, agriculture is becoming increasingly vulnerable, especially during unexpected public health emergencies such as pandemics. Fortunately, the adaptability of terrace vegetables cultivation presents an opportunity to grow horticultural crops in residential spaces, bringing numerous benefits to citizens, including enhanced nutrition and recreational engagement in the cultivation process. Although certain planting skills and equipment have been developed, the citizens tend to sow some seeds with unknown pedigree, it is rare to find new plant varieties specifically bred for cultivation as terrace vegetables. To expand the genetic basis of new breeding materials, elite parents, and varieties (pre-breeding) for terrace cultivation, this review not only discusses the molecular breeding strategy for the identification, creation, and application of rational alleles for improving horticultural characteristics including plant architecture, flavor quality, and ornamental character, but also assesses the potential for terrace cultivation of some representative vegetable crops. We conclude that the process of pre-breeding specifically for terrace cultivation environments is vital for generating a genetic basis for urban terrace vegetable crops.

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“…Although many ideotypes can be enumerated dependent on specific demands, the likelihood of achieving a specific GSL engineering goal will depend on the feasibility and complexity of applying available biotechnological approaches. Gene editing or transgenic approaches are available for GSL engineering, which have been demonstrated for most Brassica species ( Li et al., 2021a ; Neequaye et al., 2021 ; He et al., 2022 ; Tan et al., 2022 ) ( Tables 2 and 3 ; Supplemental Table 2 ), and are expected to become available for a wider range of Brassicaceae species ( Miao et al., 2021 ; Neequaye et al., 2021 ; Kim et al., 2022 ). In future GSL engineering work, selection of an appropriate combination of target genes involved in GSL biosynthesis, transport, and hydrolysis is an important preliminary step in the design of crop ideotypes.…”

Section: Generating Multifunctional Crops With Defined Gsl Profiles A...mentioning

confidence: 99%

Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

Qin

King

Borpatragohain

et al. 2023

Plant Communications

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Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing

Cited by 14 publications

References 40 publications

Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli

Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli

Mainstreaming production and nutrient resilience of vegetable crops in megacities: pre-breeding for terrace cultivation

Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

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