Development of Halomonas TD01 as a host for open production of chemicals

Fu, Xiaozhi; Tan, Dan; Aibaidula, Gulsimay; Wu, Qiong; Chen, Jinchun; Chen, Guoqiang

doi:10.1016/j.ymben.2014.02.006

Cited by 156 publications

(113 citation statements)

References 70 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…(Fu et al 2014;Li et al 2014), using primers P5 and P6, and employing ∼100 ng of the amplified fragment together with ∼10 ng of circularly closed pSEVA331-SLO template DNA in a primerless PCR using Q5 as described above. Subsequently, positive clones were selected simply by picking up visibly green single colonies, yielding the construct pSEVA331-SG1 (synthetic lysis operon-GFP; Fig.…”

Section: Vector Constructionmentioning

confidence: 99%

A novel cell autolysis system for cost-competitive downstream processing

Hajnal

Chen

2016

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

The industrial production of low value-added biological products poses significant challenges due to cost pressures. In recent years, it has been argued that synthetic biology approaches will lead to breakthroughs that eliminate price bottlenecks for the production of a wide range of biological products including bioplastics and biofuels. One significant bottleneck lies in the necessity to break the tough cell walls of microbes in order to release intracellular products. We here report the implementation of the first synthetic biology standard part based on the lambda phage SRRz genes and a synthetic ribosome binding site (RBS) that works in Escherichia coli and Halomonas campaniensis, which enables the producer strains to induce lysis after the addition of small amounts (1-5 %) of solvents or to spontaneously lyse during the stresses of downstream processing, and thus has the potential to eliminate the mechanical cell disruption step as both an efficiency bottleneck and a significant capex barrier when implementing downstream bioprocesses.

show abstract

Section: Vector Constructionmentioning

confidence: 99%

A novel cell autolysis system for cost-competitive downstream processing

Hajnal

Chen

2016

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Synthetic biology has found applications in the construction of novel robust industrial strains [53][54][55] possibly other fermentation processes [56,57]. In addition to providing future platforms for PHA from unrelated carbon sources, a final step in which synthetic biology should be exploited is the simplification of downstream processing, including simplified cell separation and disruption, which is the final frontier in cost-reduction with the toolbox of synthetic biology [53,54].…”

Section: Synthetic Biology and Pha Diversitymentioning

confidence: 99%

Engineering Biosynthesis Mechanisms for Diversifying Polyhydroxyalkanoates

Chen

Hajnal

et al. 2015

Trends in Biotechnology

Self Cite

120

View full text Add to dashboard Cite

“…Propionic acid can also be used in the manufacture of cellulose plastics, perfumes, herbicides and pharmaceuticals. Recently, propionic acid as the substrate for the microbial production of biodegradable and biocompatible polyesters has also been investigated (Fu et al, 2014). Currently, propionic acid is produced exclusively via Reppe process and Larson process, which rely on non-renewable petrochemical feedstocks and cause environmental pollution.…”

Section: Introductionmentioning

confidence: 99%