A method to calibrate the overlap factor of Lidar is proposed, named unmanned aerial vehicle correction (UAVC), which uses unmanned aerial vehicles (UAVs) to detect the vertical distribution of particle concentrations. The conversion relationship between the particulate matter concentration and the aerosol extinction coefficient is inverted by the high-altitude coincidence of the vertical detection profiles of the UAV and Lidar. Using this conversion relationship, the Lidar signal without the influence of the overlap factor can be inverted. Then, the overlap factor profile is obtained by comparing the signal with the original Lidar signal. A 355 nm Raman-Mie Lidar and UAV were used to measure overlap factors under different weather conditions. After comparison with the Raman method, it is found that the overlap factors calculated by the two methods are in good agreement. The changing trend of the extinction coefficient at each height is relatively consistent, after comparing the inversion result of the corrected Lidar signal with the ground data. The results show that after the continuously measured Lidar signal is corrected by the overlap factor measured by this method, low-altitude aerosol information can be effectively obtained.