Development of <i>Anncaliia algerae</i> (Microsporidia) in <i>Drosophila melanogaster</i>

Sokolova, Yuliya Y.; Weidner, Earl

doi:10.1111/jeu.12762

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…How the coils of the PT are organized in 3D and its spatial relationship to other organelles within the spore is less well understood. We used SBFSEM[ 37 ] to generate 3D reconstructions of the PT in intact dormant spores of the microsporidian species Anncaliia algerae , which can infect both invertebrate and vertebrate hosts, including humans[ 38 – 41 ]. SBFSEM allows us to automate the sectioning and imaging of a series of 50 nm slices through a block of spores in a high throughput manner[ 42 ].…”

Section: Resultsmentioning

confidence: 99%

3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery

et al. 2020

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Microsporidia, a divergent group of single-celled eukaryotic parasites, harness a specialized harpoon-like invasion apparatus called the polar tube (PT) to gain entry into host cells. The PT is tightly coiled within the transmissible extracellular spore, and is about 20 times the length of the spore. Once triggered, the PT is rapidly ejected and is thought to penetrate the host cell, acting as a conduit for the transfer of infectious cargo into the host. The organization of this specialized infection apparatus in the spore, how it is deployed, and how the nucleus and other large cargo are transported through the narrow PT are not well understood. Here we use serial block-face scanning electron microscopy to reveal the 3-dimensional architecture of the PT and its relative spatial orientation to other organelles within the spore. Using high-speed optical microscopy, we also capture and quantify the entire PT germination process of three human-infecting microsporidian species in vitro: Anncaliia algerae, Encephalitozoon hellem and E. intestinalis. Our results show that the emerging PT experiences very high accelerating forces to reach velocities exceeding 300 μm�s-1 , and that firing kinetics differ markedly between species. Live-cell imaging reveals that the nucleus, which is at least 7 times larger than the diameter of the PT, undergoes extreme deformation to fit through the narrow tube, and moves at speeds comparable to PT extension. Our study sheds new light on the 3-dimensional organization, dynamics, and mechanism of PT extrusion, and shows how infectious cargo moves through the tube to initiate infection.

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Section: Resultsmentioning

confidence: 99%

3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery

et al. 2020

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“…How the coils of the PT are organized in 3D and its spatial relationship to other organelles within the spore is less well understood. We used SBFSEM 30 to generate 3D reconstructions of the PT in intact dormant spores of the microsporidian species Anncaliia algerae, which can infect both invertebrate and vertebrate hosts including humans [31][32][33][34] . SBFSEM allows us to automate the sectioning and imaging of a series of 50 nm slices through a block of spores in a high throughput manner 35 .…”

Section: D Reconstruction Of a Algerae Sporesmentioning

confidence: 99%

3-Dimensional Organization and Dynamics of the Microsporidian Polar Tube Invasion Machinery

Jaroenlak

Cammer

Davydov

et al. 2020

Preprint

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Microsporidia, a divergent group of single-celled eukaryotic parasites, harness a specialized harpoon-like invasion apparatus called the polar tube (PT) to gain entry into host cells. The PT is tightly coiled within the transmissible extracellular spore, and is about 20 times the length of the spore. Once triggered, the PT is rapidly ejected and is thought to penetrate the host cell, acting as a conduit for the transfer of infectious cargo into the host. The organization of this specialized infection apparatus in the spore, how it is deployed, and how the nucleus and other large cargo are transported through the narrow PT are not well understood. Here we use serial block-face scanning electron microscopy to reveal the 3-dimensional architecture of the PT and its relative spatial orientation to other organelles within the spore. Using high-speed optical microscopy, we also capture and quantify the entire PT germination process in vitro. Our results show that the emerging PT experiences very high accelerating forces to reach velocities exceeding 300 μm⋅s -1 , and that firing kinetics differ markedly between species. Live-cell imaging reveals that the nucleus, which is approximately 7 times larger than the diameter of the PT, undergoes extreme deformation to fit through the narrow tube, and moves at speeds comparable to PT extension. Our study sheds new light on the 3-dimensional organization, dynamics, and mechanism of PT extrusion, and shows how infectious cargo moves through the tube to initiate infection.

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“…One group within this lineage exemplifies the generalist nature of some microsporidian species: A. algerae naturally infects a broad range of mosquito host species [44,45] and the number of insect hosts and cell lines that can be infected by A. algerae under laboratory conditions keeps growing [46][47][48]. This broad host range may in part be facilitated by an ability to survive and grow at a wide range of temperatures [46].…”

Section: Trends Trends In In Parasitology Parasitologymentioning

confidence: 99%

Microsporidia: a new taxonomic, evolutionary, and ecological synthesis

Bojko

Reinke

Stentiford

et al. 2022

Trends in Parasitology

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Development of Anncaliia algerae (Microsporidia) in Drosophila melanogaster

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 8 publications

References 29 publications

3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery

3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery

3-Dimensional Organization and Dynamics of the Microsporidian Polar Tube Invasion Machinery

Microsporidia: a new taxonomic, evolutionary, and ecological synthesis

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