Development of imidazolinone herbicide tolerant borage ( Borago officinalis L.)

Song, Dongyan; Wu, Guohai; Vrinten, Patricia; Qiu, Xiao

doi:10.1016/j.plantsci.2017.05.012

Cited by 6 publications

(5 citation statements)

References 39 publications

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“…Encouraged by these results, we further applied the high-throughput fluorogenic assay for discovery of a broad-spectrum herbicide-resistant AHAS of a common crop Soybean , where the gene disclosed herein can be introduced into a plant that (1) can prevent the plant from herbicides damage and (2) can provide a convenient and economical way to overcome weed resistance. − …”

Section: Resultsmentioning

confidence: 99%

“…The first-step decarboxylation of pyruvate is rate-limiting, where the second-step substrate-binding pocket in AHAS can accept a variety of unnatural aldehydes. , Widely distributed in plant, bacteria, fungi, and algae, but not in mammals, AHAS is considered an attractive drug target. Indeed, researchers identified AHAS in plants as a target for potent herbicides (such as sulfonylurea and imidazolinone). − However, due to the long-term application, weeds have developed high-level drug resistance because of mutations that interfere with the inhibition of AHAS. − To address the issue, there is still unmet demand on new AHAS inhibitors − or a new broad-spectrum herbicide-resistant AHAS gene that can function in crops to increase the tolerance to soil residues, − both of which suggest that a high-throughput AHAS assay would be of significant utility.…”

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confidence: 99%

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Fluorogenic Assay for Acetohydroxyacid Synthase: Design and Applications

et al. 2019

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Acetohydroxyacid synthase (AHAS) exists in plants and many microorganisms (including gut flora) but not in mammals, making it an attractive drug target. Fluorescent-based methods should be practical for high-throughput screening of inhibitors. Herein, we describe the development of the first AHAS fluorogenic assay based on an intramolecular charge transfer (ICT)-based fluorescent probe. The assay is facile, sensitive, and continuous and can be applied toward various AHASs from different species, AHAS mutants, and crude cell lysates. The fluorogenic assay was successfully applied for (1) high-throughput screening of commerical herbicides toward different AHASs for choosing matching herbicides, (2) identification of a Soybean AHAS gene with broad-spectrum herbicide resistance, and (3) identification of selective inhibitors toward intestinal-bacterial AHASs. Among the AHAS inhibitors, an active agent was found for selective inhibition of obesity-associated Ruminococcus torques growth, implying the possibility of AHAS inhibitors for the ultimate goal toward antiobesity therapeutics. The fluorogenic assay opens the door for high-throughput programs in AHAS-related fields, and the design principle might be applied for development of fluorogenic assays of other synthases.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Fluorogenic Assay for Acetohydroxyacid Synthase: Design and Applications

et al. 2019

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“…They are also important assays to identify herbicide-resistant weeds if the resistance mechanism involves a target-site mutation. Based on a Ser653Asn mutation, a genotyping assay was efficient to detect homozygous and heterozygous plants within ALS-resistant borage (Borago officinalis) populations [48]. Similarly, a genotyping assay was developed to identify resistant alleles containing double amino acid substitutions (Trp102Ile and Pro106Thr) within the tetraploid genome of glyphosate resistant Bidens subalternans populations [49].…”

Section: Genotyping Assay For Trp574leumentioning

confidence: 99%

A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil

et al. 2020

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Wild poinsettia (Euphorbia heterophylla L.) is an important weed species in southern Brazil, especially due to the evolution of multiple herbicide resistance (e.g., acetolactate synthase (ALS)- inhibitors, protoporphyrinogen oxidase inhibitors, and glyphosate). The mechanism of resistance to imazamox was investigated in two wild poinsettia populations (R1 and R2) from southern Brazil and compared to a known susceptible (S) population. Imazamox dose-response experiments revealed high levels of resistance: 45-fold and 224.5-fold based on dry biomass reduction, for R1 and R2, respectively. Extremely high concentrations of imazamox (20,000 µM) were not sufficient to provide 50% inhibition of ALS enzyme activity (I50) for R1 or R2. Hence, resistance levels were estimated to be greater than 123-fold for both populations based on in vitro ALS assays. The ALS gene from all R1 and R2 plants had a Trp574Leu mutation. A genotyping assay was developed to discriminate resistant and susceptible alleles based on the Trp574Leu mutation.

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“…Clearly, using herbicide-resistant rice varieties has been proven to be one of the most effective methods by which to eliminate WR from fields (Song et al 2017). The CPS system provides an efficient tool to selectively control WR in the post-emergence stage (Novakova 1994).…”

Section: The Successful Story Of Imi Herbicides and Clearfield Rice Amentioning

confidence: 99%

Introduction of imidazolinone herbicide and Clearfield® rice between weedy rice—control efficiency and environmental concerns

2018

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Water scarcity and increasing labor costs of rice cultivation have prompted many agro-ecosystems in the world to adopt the direct-seeded rice (DSR) method instead of the hand-transplanting method. However, there is a downside to this approach, which is the prevalence and spread of weedy rice (WR), a troublesome weed in paddy fields that has the potential to cause a 90% loss of total yield in high-infested areas. The progression, infestation, and dynamics of WR are linked to environmental circumstances, types of rice cultivar, established techniques, and field management. WR is viewed as a critical problem, as it may prove counterproductive in rice cultivation because it causes an overall increase in the production cost of paddy harvesting. For the purpose of our discussion, a method is explored that can be used to eliminate, or at least mitigate, the spread of WR, which is the Clearfield® Production System (CPS). This method consists of imidazolinone (IMI) herbicide, Clearfield® certified seeds, and the Stewardship Guide. However, use of the CPS has been known to negatively affect the environment, as it transfers resistance traits to WR, increasing IMI persistence in the cultivated soils, and contaminating soils and water with herbicide residues. These negative environmental effects could be dealt with by using integrated weed management systems (IWMS) that include the use of all viable tools and should be incorporated with the proper Stewardship Guide to reduce the growth of herbicide-resistant WR. This review aims to elucidate information pertaining to WR infestation, the characteristics thereof, sustainable techniques for WR control, IMI herbicides, and diverse methods for the extraction and determination of IMI residues in the environment. Understanding the conspecific nature of WR serves as a baseline for constructing novel WR control strategies in the future.

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Development of imidazolinone herbicide tolerant borage ( Borago officinalis L.)

Cited by 6 publications

References 39 publications

Fluorogenic Assay for Acetohydroxyacid Synthase: Design and Applications

Fluorogenic Assay for Acetohydroxyacid Synthase: Design and Applications

A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil

Introduction of imidazolinone herbicide and Clearfield® rice between weedy rice—control efficiency and environmental concerns

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