Development of immunoassays for the determination of phthalates

Li, Lei; Zhang, Mingcui

doi:10.1080/09540105.2020.1721439

Cited by 13 publications

(8 citation statements)

References 78 publications

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“…A total of 96 transparent plates (Corning) made of porous polyethene were purchased from Shanghai Bioengineering Co. Bovine serum albumin (BSA) and casein were purchased from Shanghai Bioengineering Reagents Co., Ltd. Anti-BSA antibody was obtained according to the method that we previously reported . The aqueous solutions used in the experiment were all ultrapure water (18.25 M, Millie-Q, Millipore).…”

Section: Methodsmentioning

confidence: 99%

Platinum Janus Nanoparticles as Peroxidase Mimics for Catalytic Immunosorbent Assay

Chen

Guo

et al. 2022

ACS Appl. Nano Mater.

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The catalytic efficiency of peroxidase mimic enzymes has not been substantially improved despite the wide variety of peroxidase mimic nanozymes. Here, we report a class of highly efficient peroxidase mimics, platinum Janus nanoparticles (Pt Janus NPs), which are synergy assembled from silica NPs and single-component Pt NPs. Pt Janus NPs exhibit ultrahigh catalytic efficiency, with a catalytic constant (K cat) as high as 5.6 × 105 s–1, which is about 50-fold greater than the K cat value of traditional Pt NPs and about 140-fold higher than that of HRP. Compared with the single-component Pt NPs, silica components in Pt Janus NPs could effectively adsorb the intermediates during the catalysis process, which weakened adsorption on the surface of Pt NPs. Moreover, we reveal that the silica component could be avoided by aggregation of Pt NPs, which protects the catalytic sites and improves the catalytic efficiency. We conclude that Janus NPs have a stable, flexible, and efficient structure that protects the catalytic activity of nanozymes to enhance the catalytic efficiency. The relative signal strength is 3.4-fold higher than that of Pt ELISA when Pt Janus NPs were applied to direct immunoassay.

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Section: Methodsmentioning

confidence: 99%

Platinum Janus Nanoparticles as Peroxidase Mimics for Catalytic Immunosorbent Assay

Chen

Guo

et al. 2022

ACS Appl. Nano Mater.

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“…Different kinds of medical devices for therapeutic maintenance such as tubes for circulatory fluids, blood bags, and dialysis tubing materials are also made up of plastic which makes them softer with different kinds of PVC and organic compounds. Skin absorption can occur through direct contact with cosmetics and clothing products containing phthalate [ 7 ]. In silage, DBP is almost surely contaminated due to immigration from interaction materials such as sticking films, sails collected from rivers used during manufacture, or during farm storage [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Cytotoxic, Genotoxic, and Oxidative Stress of Dibutyl Phthalate on Cultured Bovine Peripheral Lymphocytes

Ali

Sahar

Firyal

et al. 2022

Oxidative Medicine and Cellular Longevity

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Recently, there have been numerous reports showing that phthalates have negative human health impacts and may cause several diseases such as asthma, breast cancer, obesity, type II diabetes, and male infertility. Animals are also exposed to phthalates through the environment and can cause adverse health effects on them. Several studies have been found on the cytogenetic effects of dibutyl phthalate (DBP) on different organisms but no documented evidence has been found on the cytotoxic and genotoxic effects of dibutyl phthalate (DBP) on bovine cultured lymphocytes. MTT assay was performed on different series of DBP concentrations (10 μM, 20 μM, 30 μM, 50 μM, 70 μM, 100 μM). A concentration-dependent decrease in cell viability was observed by the DBP. The LD50, LD50/2, and 2 ∗ LD50 were found to be 50 μM, 30 μM, and 80 μM on bovine lymphocytes, respectively. Then, these concentrations of DBP were utilized to perform comet, micronucleus assays, and oxidative stress. A concentration-dependent increase in DNA damage, oxidative stress, and micronuclei formation was observed in lymphocytes by the DBP as compared to the control group. Highest genotoxic effects were observed at a concentration of 2 ∗ LD50. Similarly, total oxidative stress was found higher, and antioxidative stress was lower in concentration-dependent manner by the DBP. The current study revealed a significant cytotoxic, genotoxic, and oxidative stress of DBP on cultured bovine lymphocytes.

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“…We selected AgNPs and AuNPs as model antigens to immunize rabbits and validate nanoparticle-induced antibody production. They are inert and often used as models for nanovaccines. , Based on the preparation, theory, and experience with small-molecule antibodies, we first attempted to research on gold or silver nanoparticles conjugated with BSA as immunogens . The methods used to couple thioglycolic acid (TGA)-modified gold or silver nanoparticles to the BSA carrier protein are reported in the literature .…”

Section: Results and Discussionmentioning

confidence: 99%

Nanoparticle/Nanocarrier Formulation as an Antigen: The Immunogenicity and Antigenicity of Itself

Yan

Meng

et al. 2021

Mol. Pharmaceutics

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In antibody preparation, the immunogenicity of small molecules is limited due to the instability of adjuvant/hapten emulsions. Nanoparticle-based adjuvants overcome instability and effectively improve immune responses. Immunogenicity and antigenicity are fundamentally important, yet understudied, facets of nanoparticle formulations themselves. Herein, we studied the immunogenicity and antigenicity of nanoparticle formulations. In experiments in a rabbit model, simple inorganic nanoparticle (e.g., gold nanoparticle (AuNP) and silver nanoparticle (AgNP)) immunogens induced higher titers of antiserum. Moreover, several promising nanoparticle drug carrier immunogens (e.g., SiO2, oleylamine graft polysuccinimide (PSIOAm), oleylamine and N-(3-aminopropyl)imidazole cograft polysuccinimide (PSIOAm‑NAPI), Fe3O4@O-dextran, etc.) showed excellent immunogenicity. Cross-reactivity calculations revealed that the antigenicity properties of AgNP and AuNP antigens are highly size-dependent. Meanwhile, four nanoparticle drug carriers generate antibody-specific immune responses to their antigens. The reactivity of the anti-NP antibodies with nanoparticle antigens was confirmed using immunoassays. This study systematically identified the immunogenicity and antigenicity of the nanoparticle formulation itself. These findings provide insights into the immunological properties of the nanoparticle formulation itself in an organism.

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Development of immunoassays for the determination of phthalates

Cited by 13 publications

References 78 publications

Platinum Janus Nanoparticles as Peroxidase Mimics for Catalytic Immunosorbent Assay

Platinum Janus Nanoparticles as Peroxidase Mimics for Catalytic Immunosorbent Assay

Assessment of Cytotoxic, Genotoxic, and Oxidative Stress of Dibutyl Phthalate on Cultured Bovine Peripheral Lymphocytes

Nanoparticle/Nanocarrier Formulation as an Antigen: The Immunogenicity and Antigenicity of Itself

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