Development of in‐flow label‐free single molecule sensors using planar solid‐state nanopore integrated microfluidic devices

Güzel, Fatma Doğan; Miles, Benjamin N.

doi:10.1049/mnl.2018.5206

Cited by 12 publications

(7 citation statements)

References 39 publications

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“…Microfluidic devices and lab-on-a-chip systems are widely used nowadays in chemical and biological sciences [1][2][3][4] for applications ranging from the synthesis of nanoparticles and colloidal systems [5][6][7] to molecular diagnostics [8,9] and cell biology [10,11], owing to their reduced consumption of reactants [12], better control over reaction variables such as the reactant concentration and temperature [13,14], and the ability to spatially control liquid composition with cellular resolution [11,15]. Virtually any use of these devices in chemical analysis and fabrication or biological assays/bioengineering requires the ability to mix well and predictably two or more chemical or biological components [16,17].…”

Section: Introductionmentioning

confidence: 99%

Mixing Optimization in Grooved Serpentine Microchannels

2020

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Computational fluid dynamics modeling at Reynolds numbers ranging from 10 to 100 was used to characterize the performance of a new type of micromixer employing a serpentine channel with a grooved surface. The new topology exploits the overlap between the typical Dean flows present in curved channels due to the centrifugal forces experienced by the fluids, and the helical flows induced by slanted groove-ridge patterns with respect to the direction of the flow. The resulting flows are complex, with multiple vortices and saddle points, leading to enhanced mixing across the section of the channel. The optimization of the mixers with respect to the inner radius of curvature (Rin) of the serpentine channel identifies the designs in which the mixing index quality is both high (M > 0.95) and independent of the Reynolds number across all the values investigated.

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Section: Introductionmentioning

confidence: 99%

Mixing Optimization in Grooved Serpentine Microchannels

2020

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“…Consequently, the back cover structure with the equivalent effect of noise suppression was recommended for its relatively small corresponding delay. The synergistic effect of flow velocity and drift velocity exist in solid-state nanopore integrated microfluidic devices, which is a universal task for all nanopore-microfluidic devices, was presented by Guzel and coworkers [31]. The author gave the DNA capture regime as three parts: the diffusion-limited regime, transitional regime, and flow-limited regime, and they had different regions of action, which are determined by the location of the distance from the nanopore (within 500 nm or not), the driven speed of the electrical field and microfluidic flow.…”

Section: Fundamental Configuration Of a Nanopore-based Microfluidic Systemmentioning

confidence: 98%

Microfluidic Systems Applied in Solid-State Nanopore Sensors

Qiao

et al. 2020

Micromachines

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Microfluidic system, as a kind of miniature integrated operating platform, has been applied to solid-state nanopore sensors after many years of experimental study. In the process of introducing microfluidic into solid-state nanopore sensors, many novel device structures are designed due to the abundance of analytes and the diversity of detection methods. Here we review the fundamental setup of nanopore-based microfluidic systems and the developments and advancements that have been taking place in the field. The microfluidic systems with a multichannel strategy to elevate the throughput and efficiency of nanopore sensors are then presented. Multifunctional detection represented by optical-electrical detection, which is realized by microfluidic integration, is also described. A high integration microfluidic system with nanopore is further discussed, which shows the prototype of commercialization.

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“…This advanced technology benefits from the advantages of lab on‐chip technologies consisting of sophisticated, but often very basic, microfluidic channel networks. Microfluidic systems have gone through many developments to perform complex and time‐consuming biological functions in a much effective and shorter time [ 23 , 24 , 25 , 26 ]. These systems are also cost effective and automated while consuming small fluid volume [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

On‐chip label‐free impedance‐based detection of antibiotic permeation

Kaur

Ghorbanpoor

Öztürk

et al. 2021

IET Nanobiotechnology

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Biosensors are analytical tools used for the analysis of biomaterial samples and provide an understanding about the biocomposition, structure, and function of biomolecules and/or biomechanisms by converting the biological response into an electrical and/or optical signal. In particular, with the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes in the field of biosensors has been gaining widespread importance. Herein, for the rapid and label-free detection of antibiotic permeation across a membrane, a microelectrode integrated microfluidic device is presented. The integrated chip consists of polydimethylsiloxane based microfluidic channels bonded onto microelectrodes on-glass and enables us to recognize the antibiotic permeation across a membrane into the model membranes based on electrical impedance measurement, while also allowing optical monitoring. Impedance testing is label free and therefore allows the detection of both fluorescent and non-fluorescent antibiotics. As a model membrane, Giant Unilamellar Vesicles (GUVs) are used and impedance measurements were performed by a precision inductance, capacitance, and resistance metre. The measured signal recorded from the device was used to determine the change in concentration inside and outside of the GUVs. We have found that permeation of antibiotic molecules can be easily monitored over time using the proposed integrated device. The results also show a clear difference between bilayer permeation that occurs through the lipidic bilayer and porin-mediated permeation through the porin channels inserted in the lipid bilayer. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Development of in‐flow label‐free single molecule sensors using planar solid‐state nanopore integrated microfluidic devices

Cited by 12 publications

References 39 publications

Mixing Optimization in Grooved Serpentine Microchannels

Mixing Optimization in Grooved Serpentine Microchannels

Microfluidic Systems Applied in Solid-State Nanopore Sensors

On‐chip label‐free impedance‐based detection of antibiotic permeation

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