Development of In-Vitro Regeneration Protocol From Seedling Explants of Populus Gamblei Dode: A Difficult to Propagate Endemic Tree Species of Eastern Himalayas

Chandra, Puran; Thakur, Ajay; Mohapatra, K. P.; Mehra, T. S.; Kunwar, Arti; Chauhan, Monika

doi:10.21203/rs.3.rs-910342/v1

2021

DOI: 10.21203/rs.3.rs-910342/v1

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Development of In-Vitro Regeneration Protocol From Seedling Explants of Populus Gamblei Dode: A Difficult to Propagate Endemic Tree Species of Eastern Himalayas

Puran Chandra

Ajay Thakur

K. P. Mohapatra

et al.

Abstract: This is the first report of in-vitro regeneration of Populus gamblei, an endemic and rare tree of montane to subtropical forest of Eastern Himalayas. Nodal segment from coppice shoot of young headed back seedling resulted in 100 % sprouting in MS + 1.0 mg L-1 BAP. Each micro shoot proliferated and gave 7.06 micro shoots on MS + 0.50 mg L-1 BAP + 0.10 mg L-1 IBA. In vitro multiplied shoots produced 100 % rooting in ½ MS + 0.5 mg L-1 NAA. In-vitro regenerated plants recorded 80 percent survival during hardening.

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Biotechnological Aspects of Propagation of Black Poplar Hybrids “San Giorgio” and “Ghoy”

Pinchuk¹,

KLYUVADENKO²,

IVANYUK³

et al. 2022

Ukr. j. for. wood sci.

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Energy independence of the country can be solved in several ways. One of them is the creation of energy plantations of woody plants. The area increase of such plantations is limited by the insufficient amount of high-quality planting material. Hybrid plants of the genus Populus are of considerable interest when creating plantations. Given that not all hybrids are successfully propagated by conventional vegetative methods, the use of biotechnological methods, namely microclonal propagation, solves the mentioned problem. This method allows obtaining a large amount of highquality, uniform planting material. The purpose of this study was to develop the technology of microclonal propagation of black poplar hybrids ‘San Giorgio’ and ‘Ghoy’. The study used generally accepted biotechnological methods in the author’s modification at all stages of obtaining planting material of regenerating plants of poplar hybrids. Studies have established a positive effect when obtaining an aseptic culture of sterilising substances 0.1% AgNO3, 2.5 and 1.25% NaClO, 0.05% sodium merthiolate and 0.1% HgCl2. The largest number of aseptic viable explants was obtained by cultures using a 0.05% solution of sodium merthiolate after exposure for 5 and 10 minutes. When rooting microshoots on a hormone-free nutrient medium ½ MS and with the addition of 0.1-1.0 mg/l IBA, it was established that the best parameters for rooting microshoots are on a hormone-free nutrient medium ½ MS. Adaptation was carried out for 4 weeks in a greenhouse on a substrate that included components of peat:sand:perlite in a ratio of 1:1:1. The viability of regenerating plants of black poplar hybrids ‘San Giorgio’ and ‘Ghoy’ was more than 91%. The conducted research and the obtained results will be useful for producers of planting material and the scientific environment, which develop renewable energy sources through the creation of energy plantations of fast-growing woody plants

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Biotechnological Aspects of Propagation of Black Poplar Hybrids “San Giorgio” and “Ghoy”

Pinchuk¹,

KLYUVADENKO²,

IVANYUK³

et al. 2022

Ukr. j. for. wood sci.

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Development of In-Vitro Regeneration Protocol From Seedling Explants of Populus Gamblei Dode: A Difficult to Propagate Endemic Tree Species of Eastern Himalayas

Cited by 1 publication

References 0 publications

Biotechnological Aspects of Propagation of Black Poplar Hybrids “San Giorgio” and “Ghoy”

Biotechnological Aspects of Propagation of Black Poplar Hybrids “San Giorgio” and “Ghoy”

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