2013
DOI: 10.9775/kvfd.2013.9348
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Development of Indirect ELISA for the Diagnosis of Bovine Hypodermosis (Hypoderma lineatum) in the Cattle of Subtropical Region of Pakistan

Abstract: The aim of this study was to develop an indirect ELISA for the detection of Hypoderma lineatum antibodies and to determine the influence of seroepidemiological factors on the seroprevalence of bovine hypodermosis in cattle of subtropical region of Pakistan. For this purpose a total of 1000 blood samples were taken from twenty eight villages of Rawalpindi, Attock, Chakwal and Jhelum districts. First instar larvae (L 1 ) of the warble fly were collected from the surrounding abattoirs to obtain the antigen (HyC) … Show more

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Cited by 4 publications
(6 citation statements)
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“…In southwestern Spain, the maximum annual temperatures are 20-21ºC, the maximum summer temperatures are 29-30ºC, which favors faster development and earlier appearance of the disease in the course of the year (7,17).…”
Section: Discussionmentioning
confidence: 99%
“…In southwestern Spain, the maximum annual temperatures are 20-21ºC, the maximum summer temperatures are 29-30ºC, which favors faster development and earlier appearance of the disease in the course of the year (7,17).…”
Section: Discussionmentioning
confidence: 99%
“…Hypodermin C was purified from Hypoderma lineatum first instars obtained from cattle slaughtered in local slaughterhouses, Rawalpindi [ 28 ].…”
Section: Methodsmentioning
confidence: 99%
“…For anti- P. silenus antibody detection based on the HyC antigen, an indirect ELISA was standardized using 100 μL (2 μL/mL) HyC antigen and 50 μL (1:10,000) immuno-conjugate (horseradish-peroxidase conjugated) rabbit anti-bovine Ig G (H + L) (Geneway: GWBC158CO) in each well. The ELISA protocol, with minor modifications, was used for the bioassay [ 9 , 28 ]. The OD was measured at a wavelength of 492 nm using a FLUOstar OPTIMA spectrophotometer.…”
Section: Methodsmentioning
confidence: 99%
“…Blood samples were taken, and serum samples were tested for antibodies against Hypoderma spp. using the enzyme-linked immunosorbent assay (ELISA) method [19]. During the village visit, herd type (dairy, mixed, or beef herd) and size (number of cattle on the premises) were also recorded.…”
Section: Location Survey Design and Sample Sizementioning
confidence: 99%