Development of instrumentation for routine ToF-SIMS imaging analysis of biological material

Cliff, B.; Lockyer, N.; Corlett, Clive A.; Vickerman, John C.

doi:10.1016/s0169-4332(02)00807-3

Cited by 15 publications

(8 citation statements)

References 2 publications

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“…The second source of variation, environmental contamination, is most frequently demonstrated as differences in the amount of PDMS among the embryos. In this experiment, there was a higher concentration of PDMS in embryos 2 and 3 (sections [4][5][6][7][8][9]. The PDMS appears to have been introduced into the sample before sectioning and deparaffinization because all of the samples were handled and processed at the same time, under identical conditions.…”

Section: Reproducibility and Stability Of The Tof-sims Methodsmentioning

confidence: 94%

“…Using ToF-SIMS technology, several groups have been successful in identifying intracellular distributions of specific biological ions such as sodium, potassium, calcium and membrane lipid fragments [2][3][4][5][6][7][8][9][10][11]. Quong et al showed that in human breast cells exposed to the carcinogen PhIP, the target compound was found in detectable amounts within the outer leaflet membrane of the cells [12].…”

Section: Introductionmentioning

confidence: 99%

“…Quong et al showed that in human breast cells exposed to the carcinogen PhIP, the target compound was found in detectable amounts within the outer leaflet membrane of the cells [12]. Similarly, analysis of yeast cells that had been exposed to the drug clofazimine demonstrated the presence of the drug within the cells [8].…”

Section: Introductionmentioning

confidence: 99%

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Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Lü

Kulp

et al. 2007

International Journal of Mass Spectrometry

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Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens. Published by Elsevier B.V.

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Section: Reproducibility and Stability Of The Tof-sims Methodsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Lü

Kulp

et al. 2007

International Journal of Mass Spectrometry

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“…There are three main ionization methods used for MSI: MALDI [18,19], secondary ion MS (SIMS) [20–22] and DESI [23,24]. …”

Section: Introductionmentioning

confidence: 99%

Challenges and Recent Advances in Mass Spectrometric Imaging of Neurotransmitters

Gemperline

Chen

2014

Bioanalysis

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Mass spectrometric imaging (MSI) is a powerful tool that grants the ability to investigate a broad mass range of molecules, from small molecules to large proteins, by creating detailed distribution maps of selected compounds. To date, MSI has demonstrated its versatility in the study of neurotransmitters and neuropeptides of different classes toward investigation of neurobiological functions and diseases. These studies have provided significant insight in neurobiology over the years and current technical advances are facilitating further improvements in this field. neurotransmitters, focusing specifically on the challenges and recent Herein, we advances of MSI of neurotransmitters.

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“…Cryo-ToF-SIMS on frozen-hydrated samples has rarely been employed, especially in plant physiology. So far, attempts to implement an uninterrupted cryochain were successful only in a few cases of biological suspensions (Colliver et al, 1997;Cliff et al, 2003) and only in three cases for the analysis of plant tissues (Dérue et al, 1999(Dérue et al, , 2006aDickinson et al, 2006). First successful cryo-SIMS studies on frozen-hydrated plant tissues used stems of flax (Linum usitatissimum) to localize sodium (Na), magnesium (Mg), calcium (Ca), and potassium (K; Dérue et al, 2006a) and leaf blades of Pteris vittata to map arsenic (Dickinson et al, 2006).…”

mentioning

confidence: 99%

Imaging Nutrient Distributions in Plant Tissue Using Time-of-Flight Secondary Ion Mass Spectrometry and Scanning Electron Microscopy

Metzner¹,

Schneider²,

Breuer³

et al. 2008

Plant Physiology

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A new approach to trace the transport routes of macronutrients in plants at the level of cells and tissues and to measure their elemental distributions was developed for investigating the dynamics and structure-function relationships of transport processes. Stem samples from Phaseolus vulgaris were used as a test system. Shock freezing and cryo-preparation were combined in a cryogenic chain with cryo-time-of-flight secondary ion mass spectrometry (cryo-ToF-SIMS) for element and isotope-specific imaging. Cryo-scanning electron microscopy (cryo-SEM) was integrated into the cryogenic workflow to assess the quality of structural preservation. We evaluated the capability of these techniques to monitor transport pathways and processes in xylem and associated tissues using supplementary sodium (Na) and tracers for potassium (K), rubidium (Rb), and 41 K added to the transpiration stream. Cryo-ToF-SIMS imaging produced detailed mappings of water, K, calcium, magnesium, the K tracers, and Na without quantification. Lateral resolutions ranged from 10 mm in survey mappings and at high mass resolution to approximately 1 mm in high lateral resolution imaging in reduced areas and at lower mass resolution. The tracers Rb and 41 K, as well as Na, were imaged with high sensitivity in xylem vessels and surrounding tissues. The isotope signature of the stable isotope tracer was utilized for relative quantification of the 41 K tracer as a fraction of total K at the single pixel level. Cryo-SEM confirmed that tissue structures had been preserved with subcellular detail throughout all procedures. Overlays of cryo-ToF-SIMS images onto the corresponding SEM images allowed detailed correlation of nutrient images with subcellular structures.

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Development of instrumentation for routine ToF-SIMS imaging analysis of biological material

Cited by 15 publications

References 2 publications

Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Challenges and Recent Advances in Mass Spectrometric Imaging of Neurotransmitters

Imaging Nutrient Distributions in Plant Tissue Using Time-of-Flight Secondary Ion Mass Spectrometry and Scanning Electron Microscopy

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