Development of Iron-Doped Silicon Nanoparticles As Bimodal Imaging Agents

Singh, Mukesh Kumar; Atkins, Tonya M.; Muthuswamy, Elayaraja; Kamali, Saeed; Tu, Chuqiao; Louie, Angelique Y.; Kauzlarich, Susan M.

doi:10.1021/nn301536n

Cited by 63 publications

(72 citation statements)

References 63 publications

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“…Alamar Blue assay is an ideal approach to quantitatively measure proliferation and metabolic status of cells, especially for cells cultured in suspension (such as MEL cells used in the current study). 27,28 Similar to the results of cell number counting, the Alamar Blue assay suggested that the concentration of half maximal inhibition on cell viability was greater than 10 μg/mL ( Figure 5B, P < 0.01). In contrast to nAg, MEL cells are fairly vulnerable to Ag ions (in AgNO 3 ) at even very low concentrations (0.08 μg/mL), with significant reduction in cell viability in comparison to the control ( Figure 5C, P < 0.05), and cell viability was dramatically impaired by Ag ions at 1.2 μg/mL ( Figure 5C, P < 0.001).…”

Section: Resultssupporting

confidence: 71%

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

et al. 2013

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Due to its antimicrobial activity, nanosilver (nAg) has become the most widely used nanomaterial. Thus far, the mechanisms responsible for nAg-induced antimicrobial properties and nAg-mediated toxicity to organisms have not been clearly recognized. Silver (Ag) ions certainly play a crucial role, and the form of nanoparticles can change the dissolution rate, bioavailability, biodistribution, and cellular uptake of Ag. However, whether nAg exerts direct "particle-specific" effects has been under debate. Here we demonstrated that nAg exhibited a robust inhibition on RNA polymerase activity and overall RNA transcription through direct Ag binding to RNA polymerase, which is separated from the cytotoxicity pathway induced by Ag ions. nAg treatment in vitro resulted in reduced hemoglobin concentration in erythroid cells; in vivo administration of nAg in mice caused profound reduction of hemoglobin content in embryonic erythrocytes, associated with anemia in the embryos.Embryonic anemia and general proliferation deficit due to the significant inhibition on RNA synthesis, at least partially, accounted for embryonic developmental retardation upon nAg administration. To date, there is no conclusive answer to the sources of nAg-mediated toxicity: Ag ions or "particlespecific" effects, or both. We here demonstrated that both Ag ions and nAg particles simultaneously existed inside cells, demonstrating the "Trojan horse" effects of nAg particles in posing biological impacts on erythroid cells. Moreover, our results suggested that "particle-specific" effects could be the predominant mediator in eliciting biological influences on erythroid cells under relatively low concentrations of nAg exposure. The combined data highlighted the inhibitory effect of nAg on RNA polymerase activity through a direct reciprocal interaction.

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Section: Resultssupporting

confidence: 71%

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

et al. 2013

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“…6 Silicon nanoparticles were either surface oxidized to SiO x upon aging in airsaturated aqueous suspensions to yield SiO x -SiNPs and SiO x -Fe-SiNPs, or surface modified to obtain propylamine-terminated particles, PA-SiNPs and PA-Fe-SiNPs.…”

Section: Resultsmentioning

confidence: 99%

“…4 Further approaches involved the combination in a single particle of silicon semiconductor materials with iron oxides 5 and the synthesis of iron-doped silicon nanoparticles obtained from iron-doped sodium silicide precursors (xFe:Na 4 Si 4 ). 6 The latter authors reported an extreme loss of photoluminescence with increasing iron dopant concentrations in the initial mixture. 4 Herein, we investigate the effect of iron on the photoluminescence, singlet oxygen generation, and cytotoxicity towards rat glioma C6 cells of iron-containing SiNPs (FeSiNPs) in an attempt to further understand the potential use of these particles as optical sensors and therapeutic agents in biological systems.…”

Section: Introductionmentioning

confidence: 96%

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Impact of Iron Incorporation on 2–4 nm Size Silicon Nanoparticles Properties

et al. 2015

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Iron-containing silicon nanoparticles were synthesized in an attempt to understand the effect of iron on the silicon nanoparticle (SiNP) photoluminescence and singletoxygen generation capacity. A wet chemical oxidation procedure of the sodium silicide precursor, obtained from the thermal treatment in anaerobic conditions of a mixture of sodium, silicon, and an iron (III) organic salt under anaerobic conditions, was employed. Surface-oxidized and propylamine-terminated SiNPs were characterized using high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, time-resolved and steady-state photoluminescence, and time-correlated fluorescence anisotropy. Based on differences in the morphology, crystal structure, density, and photoluminescence spectrum, two distinct types of SiNPs were identified in a given synthesis batch: iron-free and iron-containing SiNPs. The results show that iron is inhomogeneously incorporated in the SiNPs leading to an efficient photoluminescence quenching. Emission arrives mainly from 2 nm size iron-free SiNPs.The nanoparticles were shown to generate singlet oxygen ( 1 O 2 ) upon 355 nm irradiation, though they were able to quench 1 O 2 . Analysis of cytotoxicity using MTT assay on rat glioma C6 cells showed a strong dependence on the nature of the surface groups, as 100 g/ml of propylamine-terminated iron-containing SiNPs leads to 85% decrease in cell viability while equal amounts of surface oxidized particles induced a 35% of cell death.

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“…The fluorescent quantum yields are 1.4% (before wash), 1.7% (first wash), 1.8% (second wash) and 2.0% (third wash). Singh et al [23] reported similar results with undoped SiNPs which exhibit higher fluorescent quantum yields than iron-doped SiNPs.…”

Section: Resultsmentioning

confidence: 59%

Silicon nanoparticles synthesised through reactive high-energy ball milling: enhancement of optical properties from the removal of iron impurities

Kuang

Mitchell

Fink

2014

Journal of Experimental Nanoscience

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Alkyl-terminated silicon nanoparticles (SiNPs) were prepared through reactive high-energy ball milling with contamination of iron from the steel milling materials. Iron impurities in the form of iron nanoparticles cause a decrease of photoluminescence intensity and an increase of the UV absorption. The iron impurities were removed either by gel permeation chromatography separation or by treatment with an aqueous HCl solution. The photoluminescence properties of the SiNPs were enhanced after the removal of iron. Transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and proton nuclear magnetic resonance were used to determine morphology, elemental composition and surface passivation of SiNPs.

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Development of Iron-Doped Silicon Nanoparticles As Bimodal Imaging Agents

Cited by 63 publications

References 63 publications

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

Silver Nanoparticles Induced RNA Polymerase-Silver Binding and RNA Transcription Inhibition in Erythroid Progenitor Cells

Impact of Iron Incorporation on 2–4 nm Size Silicon Nanoparticles Properties

Silicon nanoparticles synthesised through reactive high-energy ball milling: enhancement of optical properties from the removal of iron impurities

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