Development of isolation and cultivation method for outer root sheath cells from human hair follicle and construction of bioartificial skin

Seo, Young-Kwon; Lee, Doo-Hoon; Shin, Youn-Ho; You, Bo-Young; Lee, Kyung-Mi; Song, Kwang Yong; Seo, Seong-Jun; Whang, Sung-Joo; Kim, Young‐Jin; Yang, Eun-Kyung; Park, Chang-Seo; Chang, Ih-Seop; Park, Jung-Keug

doi:10.1007/bf02940272

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…The cells exhibited a spindle shape, with the average cell size of hUC-MSCs being smaller than that of the others. Isolated MSCs differentiated into adipocytes, chondrocytes and osteoblasts, and ORSCs exhibited a cobblestone-like morphology as previously described [32,33] (data not shown).…”

Section: Cell Isolation and Cultivation Of Mesenchymal Stem Cells (Mssupporting

confidence: 55%

“…Hair follicles at anagen (growth phase) state were selected under a microscope. To isolate ORSCs, our previous protocol was modified by performing two-step enzymatic digestions [32,33]. Briefly, isolated hair follicles were loosened by incubating them with 1.2 units/ml dispase (Gibco BRL) in PBS for 16 h at 4 8C.…”

Section: Isolation and Cultivation Of Outer Root Sheath Cells (Orscs)mentioning

confidence: 99%

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Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication

Yoo

Shin

Yoon

et al. 2010

Journal of Dermatological Science

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Section: Cell Isolation and Cultivation Of Mesenchymal Stem Cells (Mssupporting

confidence: 55%

Section: Isolation and Cultivation Of Outer Root Sheath Cells (Orscs)mentioning

confidence: 99%

Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication

Yoo

Shin

Yoon

et al. 2010

Journal of Dermatological Science

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“…In addition, bovine pituitary extract (BPE) was found to be necessary for ORSCs to proliferate and form colonies under serum-free, feeder layer-free culture conditions, but the use of type I collagen as a substratum had no positive effects. Moreover, ORSCs under BPE-added conditions contained stem/progenitor cells that expressed beta 1-integrin, CK19, and CD34 [25,26].…”

Section: Outer Root Sheath Cells (Orscs)mentioning

confidence: 99%

Hair follicular cell/organ culture in tissue engineering and regenerative medicine

Yoo¹,

Shin²,

Yoon³

et al. 2010

Biochemical Engineering Journal

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“…The hair follicle is divided into two major classes by epidermal tissues and dermal tissues [1]. The epidermal tissues of hair follicles include the outer root sheath (ORS) and the matrix, and it has been reported that epidermal stem cells are present in the ORS layer [2][3][4]. The dermal tissue of hair follicles that originate from the mesenchyme contain a dermal papilla (DP) and a dermal sheath (DS), which have a cell pattern similar to dermal fibroblasts [5].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the reconstruction of dermal papilla like tissues employing umbilical cord mesenchymal stem cells

Yoo

Shin

Yoon

et al. 2010

Biotechnol Bioproc E

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Alopecia is not life threatening, but patients who undergo alopecia often experience severe mental stress. In addition, the number of individuals afflicted by alopecia has been increasing steadily. The most effective treatment of alopecia developed to date is auto hair transplantation. To overcome the limitations associated with current therapies for the treatment of alopecia, many researchers have attempted to revive hair follicles by áå=îáíêç culture of hair follicle cells and subsequent implantation in the treatment area. Previously, we demonstrated that umbilical cord-derived mesenchymal stem cells (UC-MSCs) could be isolated and expanded successfully from the Wharton's Jelly. Cultureexpanded UC-MSCs formed aggregates similar to native dermal papilla (DP) in special media (DPFM) and reconstructed dermal papilla like tissues (DPLTs) could induce new hair follicles. The purpose of the present study was to optimize the reconstruction of DPLTs. As in the case of MSCs, when compared to differentiated cells, DPLTs require an additional step to induce differentiation into dermal papilla cells. However, it is necessary to use hepatocyte growth factor (HGF) in the differentiation step, which is relatively expensive. To reduce the expenses associated with cell therapy using MSCs, it is necessary to optimize this differentiation step. To accomplish this, we evaluated the effects of cell inoculation density and growth factors during differentiation. © KSBB hÉóïçêÇëW=ãÉëÉåÅÜóã~ä=ëíÉã=ÅÉääI=Ü~áê=ÑçääáÅäÉI=ÇÉêã~ä=é~éáää~I=ÅÉää=íÜÉê~éóI=ìãÄáäáÅ~ä=ÅçêÇ= = = = =

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Development of isolation and cultivation method for outer root sheath cells from human hair follicle and construction of bioartificial skin

Cited by 9 publications

References 26 publications

Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication

Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication

Hair follicular cell/organ culture in tissue engineering and regenerative medicine

Optimization of the reconstruction of dermal papilla like tissues employing umbilical cord mesenchymal stem cells

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