Development of liquid chromatographic method for the analysis of kanamycin residues in varicella vaccine using phenylisocyanate as a derivatization reagent

Hydrophilic interaction chromatography combined with tandem-mass spectrometry to determine six aminoglycosides in serum

“…A LC-MS method for the determination of kanamycin using phenylisocyanate as a derivatisation reagent was described [15]. The determinations of aminoglycosides in animal tissues, of gentamicin in wastewater and of tobramycin in serum by ion pair chromatography with volatile reagents and MS detection were reported in literature [16][17][18][19][20].…”

Reversed‐phase liquid chromatographic method for the analysis of aminoglycoside antibiotics using pre‐column derivatization with phenylisocyanate

“…Few gas chromatographic methods have been published (Petz et al, 1998;Soback and Isoherranen, 2000), most of them requiring trimethylsilylation to derivatize hydroxyl as well as amino groups. Previously, we published reversed-phase liquid chromatographic (RP-LC) of kanamycin using phenylisocyanate as a derivatization reagent in varicella vaccine (Kim et al, 2001), so the previous method applied to several aminoglycosides including kanamycin, neomycin and gentamicin. In this study, we describe a validated RP-LC method for the aminoglycoside antibiotics using precolumn derivatization with phenylisocyanate.…”

HPLC-MS/MS of Highly Polar Compounds

“…Another derivatization agent used for the RP-HPLC analysis of aminoglycoside antibiotics was phenylisocyanate in the presence of triethylamine. Phenylisocianate groups easily react with amino groups of aminoglycosides like kanamicin, neomycin and gentamicin (Kim et al, 2001 and; the complex structures were confirmed by ESI-MS. Clarot et al (2005) described a method for the determination of neomycin sulphate, framycetin sulphate and other related compounds by evaporative light scattering detection (ELSD) thus avoiding the need of derivatization for detection. The chromatographic separation was performed on a Polaris C18 column using a mobile phase with 170 mM trifluoroacetic acid; in this case trifluoroacetate ions act like counter-ions forming ion pairs with aminoglycosides.…”