Hydrophilic interaction chromatography combined with tandem-mass spectrometry to determine six aminoglycosides in serum

Abstract

A specific and automated method was developed to quantitate the aminoglycosides amikacin, gentamicin, kanamycin, neomycin, paromomycin, and tobramycin simultaneously in human serum. Samples were prepared with an automated solid phase extraction (SPE). The hydrophilic interaction chromatography (HILIC) was used for separation of analytes from endogenous compounds and baseline separation. The aminoglycosides were detected with electrospray ionisation tandem mass spectrometry (ESI-MS-MS). Using a volume of 500 microl biological sample the lower limits of quantification were 100 ng/ml or better. The described HILIC-MS-MS method is suitable for therapeutic drug monitoring and for clinical and pharmcokinetical investigations of the aminoglycosides.

Challenges in the determination of aminoglycoside antibiotics, a review

“…Then the cartridge was washed using 1 mL of 50% methanol in 9% formic acid and 0.7 mL water and finally eluted with 0.8 mL 50% methanol in 25% ammonia. The elute was then evaporated to dryness at 70 o C and reconstituted into mobile phase [60].…”

“…The optimal mobile phase was composed of A (0.2% formic acid in acetonitrile) and B (175 mM ammonium formate ) at [122]. A similar approach was applied in the simultaneous determination of amikacin, gentamicin, kanamycin, neomycin, paromomycin, and tobramycin in human serum [60]. The use of a ZIC column was also reported for the determination of AGs in food of animal origin [123].…”

Efficient hydrophilic interaction liquid chromatography–tandem mass spectrometry for the multiclass analysis of veterinary drugs in chicken muscle

“…Consequently, HILIC makes the separation of highly polar compounds easier and increases the chances for simultaneous analysis from less-polar to high polar compounds. Many applications of HILIC have been reported for the analysis of polar compounds including amino acids, peptides and carbohydrates [23][24][25], toxins [26], sugars [27], counter-ions [28] and veterinary drugs [29,30].…”

Hydrophilic Interaction Chromatography (HILIC) in the Analysis of Relevant Quality and Safety Biochemical Compounds in Meat, Poultry and Processed Meats

“…HILIC methodology has been widely applied during the last decades in the analysis of compounds of interest in food (Schlichtherle-Cerny et al 2003;Fenaille et al 2004;de Person et al 2005;Rotzoll et al 2005) and has also increased its popularity during the last decades in the analysis of drugs (Oertel et al 2004a;Peru et al 2006;Zheng et al 2008;Ishii et al 2008;Kesiunaite et al 2008;Kovalova et al 2009) and contaminant residues (Dixon et al 2004;Zywicki et al 2005;Lojkova et al 2006;Chung et al 2007;Andersen et al 2008;Gianotti et al 2008;Jiang and Ihunegbo 2009) in different types of foods. In this manuscript, we review the recent developments on HILIC chromatography applied to the analysis of certain biochemical compounds and antibiotic residues in the meat field.…”