Development of Lucilia cuprina: Correlation of biochemical and morphological events

Barritt, Laura C.; Birt, L.M.

doi:10.1016/0022-1910(71)90018-7

Cited by 27 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But, unlike Barrit and Birt [9], we did not find any internal leg muscles or head and abdominal muscles before the third quarter of development. Barrit and Birt [9] reported that a considerable number of both abdominal and head fat body cells found in the freshly emerged adult insect were of larval origin. We found clearly distinct fat bodies in the series 4 specimen shortly before emergence, but since the lower resolution of micro-CT compared to the traditional histological techniques employed by Barrit and Birt [9] does not allow us to trace individual cells, we are unable to determine whether the individual fat cells that presumably make up the fat bodies are novel or of larval origin.…”

Section: Discussioncontrasting

confidence: 73%

“…Our results show both similarities and differences when compared to previous studies by Barrit and Birt [9] and Greenberg [2]. As in both Barrit and Birt [9] and Greenberg [2], we find a distinction between the three principal body sections in the first quarter of development.…”

Section: Discussionsupporting

confidence: 67%

“…Like Barrit and Birt [9], we also found that the insect is free of the pupal cuticle at this stage, and that the ''mid gut'' (archenteron in the present paper) is closed off at each end and occupies roughly a third of the length of the insect. But, unlike Barrit and Birt [9], we did not find any internal leg muscles or head and abdominal muscles before the third quarter of development. Barrit and Birt [9] reported that a considerable number of both abdominal and head fat body cells found in the freshly emerged adult insect were of larval origin.…”

Section: Discussionmentioning

confidence: 58%

“…As in both Barrit and Birt [9] and Greenberg [2], we find a distinction between the three principal body sections in the first quarter of development. Like Barrit and Birt [9], we also found that the insect is free of the pupal cuticle at this stage, and that the ''mid gut'' (archenteron in the present paper) is closed off at each end and occupies roughly a third of the length of the insect. But, unlike Barrit and Birt [9], we did not find any internal leg muscles or head and abdominal muscles before the third quarter of development.…”

Section: Discussionmentioning

confidence: 91%

“…Levy and Bautz [8] illustrate the potential of this method by studying the structural changes in salivary glands of larval cells in Calliphora vicina during pupariation. Barritt and Birt [9] provide a comprehensive description of the morphological changes, at the cellular level, that take place during metamorphosis in Lucilia cuprina, in relation to time at 30 8C, using histology. However, the most comprehensive study of blowfly development is still Lowne's seminal work from more than a century ago [10,11].…”

mentioning

confidence: 99%

See 4 more Smart Citations

Virtual forensic entomology: Improving estimates of minimum post-mortem interval with 3D micro-computed tomography

Richards

Simonsen

Abel

et al. 2012

Forensic Science International

View full text Add to dashboard Cite

We demonstrate how micro-computed tomography (micro-CT) can be a powerful tool for describing internal and external morphological changes in Calliphora vicina (Diptera: Calliphoridae) during metamorphosis. Pupae were sampled during the 1st, 2nd, 3rd and 4th quarter of development after the onset of pupariation at 23 °C, and placed directly into 80% ethanol for preservation. In order to find the optimal contrast, four batches of pupae were treated differently: batch one was stained in 0.5M aqueous iodine for 1 day; two for 7 days; three was tagged with a radiopaque dye; four was left unstained (control). Pupae stained for 7d in iodine resulted in the best contrast micro-CT scans. The scans were of sufficiently high spatial resolution (17.2 μm) to visualise the internal morphology of developing pharate adults at all four ages. A combination of external and internal morphological characters was shown to have the potential to estimate the age of blowfly pupae with a higher degree of accuracy and precision than using external morphological characters alone. Age specific developmental characters are described. The technique could be used as a measure to estimate a minimum post-mortem interval in cases of suspicious death where pupae are the oldest stages of insect evidence collected.

show abstract

Section: Discussioncontrasting

confidence: 73%

Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

See 3 more Smart Citations

Virtual forensic entomology: Improving estimates of minimum post-mortem interval with 3D micro-computed tomography

Richards

Simonsen

Abel

et al. 2012

Forensic Science International

View full text Add to dashboard Cite

show abstract

Comparative study of hemocytes and associated cells of some medically important dipterans

Kaaya

Ratcliffe

1982

Journal of Morphology

View full text Add to dashboard Cite

The aim of this work is to study, characterize, and compare different morphological types of hemocytes of Glossina austeni, G. morsitans, Calliphora erythrocephala, Stomoxys calcitrans, Lucilia sericata, Aedes aegypti, and Culex quinquefasciatus. This information is intended to provide a basis for future studies of the cellular defense mechanisms of these dipterans. Seven morphological types of hemocytes were identified by phase-contrast optics: prohemocytes, plasmatocytes, thrombocytoids, granulocytes, adipohemocytes, oenocytoids, and spindle cells of various sizes. Adipohemocytes are difficult to distinguish from both fat body cells and granulocytes. All seven cell types are not present in every species. For example, thrombocytoids and spindle cells were not found in A. aegypti or C. quinquefasciatus, and oenocytoids were observed only in A. aegypti, C. quinquefasciatus, and in C. erythrocephala. In addition to the hemocytes, fat body cells and nephrocytes are also freely present in the hemolymph of some species but may have gained access to the blood during the bleeding process. With electron microscopy and with thick plastic sections of G. austeni hemolymph, only nephrocytes, plasmatocytes, granulocytes, and spindle cells were identified with any certainty, and the large spindle cells are morphologically very different from those found in the other dipterans. They are rigid cells supported by microtubules running throughout their entire length. These hemocytes are present in large numbers only in newly emerged flies, they are absent in larvae and young pupae, and are rare in old adults. Their disappearance from the hemolymph of newly emerged Glossina appears to be a result of phagocytosis by the plasmatocytes.

show abstract

Chemistry and Physiology of Ecdysoids

Sláma

Romaňuk²,

Šorm³

1974

Insect Hormones and Bioanalogues

View full text Add to dashboard Cite

Development of Lucilia cuprina: Correlation of biochemical and morphological events

Cited by 27 publications

References 29 publications

Virtual forensic entomology: Improving estimates of minimum post-mortem interval with 3D micro-computed tomography

Virtual forensic entomology: Improving estimates of minimum post-mortem interval with 3D micro-computed tomography

Comparative study of hemocytes and associated cells of some medically important dipterans

Chemistry and Physiology of Ecdysoids

Contact Info

Product

Resources

About