Development of magnetism‐assisted in‐tube solid phase microextraction of phenolic acids in fruit juices prior to high‐performance liquid chromatography quantification

Abstract

Magnetism-assisted in-tube solid phase microextraction based on porous monolith mingled with Fe 3 O 4 nanoparticles was developed for capture of phenolic acids in fruit juices. First, poly (1-allyl-3-methylimidazolium bis [(trifluoro methyl) sulfonyl] imide-co-ethylene dimethacrylate) monolith embedded with Fe 3 O 4 nanoparticles was facile fabrication in a capillary and employed as microextraction column. Subsequently, a magnetic coil adopted to produce variable magnetic fields during extraction stage was twined on the microextraction column. The analytes contents in eluant were quantified by high performance liquid chromatogram with diode array detector. Various parameters affecting the extraction performance were inspected and optimized in detail. Results revealed that the exertion of magnetic fields in adsorption and desorption steps enhanced the extraction efficiencies of analytes from 44.9-64.0% to 78.6-87.1%. Under the optimal extraction factors, the limits of detection were between 0.012 and 0.061 μg/L, relative standard deviations for precision in terms of intra-and inter-day assay variability ranged from 1.9 to 9.8%. The introduced approach was successfully applied to simultaneously quantify the contents of five analytes in real fruit juices with satisfying fortified recoveries (80.1-116%). The obtained results well demonstrate the promising potential of the developed method in the highly sensitive quantification of trace phenolic acids in complex samples.

Hydroxyl‐rich ferrofluid for efficient liquid phase microextraction of cinnamic acid derivatives in traditional Chinese medicine

“…The analytical features of the proposed HRFF-LPME were compared with those of previously reported methods [25][26][27][28][29][30][31][32][33][34][35][36] for the analysis of cinnamic acid derivatives in dif-ferent sample matrices. It can be seen in Table S4, that the amount of extractant required in the proposed approach is lower than that of other studies except hollow fiber liquid phase microextraction [28,29] and magnetic solid phase extraction based on layered double hydroxide coated MNPs [34].…”

The Research Progress of Extraction, Purification and Analysis Methods of Phenolic Compounds from Blueberry: A Comprehensive Review

“…Increased the analyte extraction efficiency from 44.9-64.0% to 78.6-87.1% [86] RP-HPLC Has good linearity and precision and can be used for commercial characterization [87] Capillary electrophoresis Hydrochloric acid concentration, 2 mol•L −1 ; temperature, 80 • C; and time 30 min; is a green extraction detection method [88] UHPLC-DAD-ESI-QTOF-MS + UHPLC-DAD Quantifies and characterizes phenolic compounds other than anthocyanins in blueberry juice and fermented wine [89] HPLC is utilized for the quantitative analysis of phenolic acids in plants. The strategy depends on the chemical characteristics of pre-detection components, extraction methods, particle size, storage time, and conditions.…”

“…Water, methanol, and acetonitrile are the most common constituents of mobile phase systems, and modifiers, such as formic acid, ammonium acetate, and acetic acid, are sometimes added to prevent peak tailing. Moreover, reversed-phase high performance liquid chromatography (RP-HPLC) also detects phenolic acids, and Padilha, C.V. et al used RP-HPLC to determine phenolic acids in Brazilian grape juice, which had good linearity and precision and was used for commercial characterizations [87].…”

Solid-Phase Microextraction Techniques and Application in Food and Horticultural Crops

“…Chen et al used in-tube solid-phase microextraction for the analysis of five phenolic acids (Figure 9) present in orange juice samples. This involved using in-tube solid-phase microextraction, with subsequent analysis using a HPLC equipped with a DAD [54]. The adsorbent coating on the inside of the capillary consisted of a poly (1-allyl-3-methylimidazolium bis [(trifluoromethyl) sulfonyl] imide-co-ethylene dimethacrylate) polymer embedded with Fe 3 O 4 nanoparticles.…”