Development of marker-free insect resistant transgenic okra (Abelmoschus esculentus L. Moench) expressing the cry1Ac gene and identification of vector backbone-free events

Deole, Satish G.; Padakipatil, Sanjeev; Sandhya, S. R.; Nanote, Asaram; Jadhav, M. S.; Bihani, Pankaj; Parimi, Srinivas; Zehr, Usha B.; Narendran, M.; Char, Bharat

doi:10.1007/s12298-021-01074-3

Cited by 1 publication

(2 citation statements)

References 17 publications

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“…Insect bioassays revealed 100 percent larval mortality without infestation in five of the transgenic events, and 5 to 10% infestation in two others, demonstrating the transgenic plants' insect resistance. 14 These plant transformation methods hold much promise to develop insect-resistant A. esculentus for target resistance against insect pests. Commercial insecticides will not be as effective as a well-designed genetic transformation method for A. esculentus.…”

Section: A G R O B a C T E R I U M T U M E F A C I E N S -M E D I A T...mentioning

confidence: 99%

“…12 An attempt has been made to transfer the Bt Cry1Ac gene into A. esculentus plantto confer a high level of resistance against natural pests and improve the crop yield without affecting the environment. 13,14,15 A. esculentus (L.) (Lady's finger) is an annual or perennial herb that grows in the tropics, subtropics and milder climates, belonging to the family Malvaceae. The plant grows to approximately 2 m tall with heart-shaped leaves that are 10-20 cm in length and breadth and palmately lobed with 5-7 lobes.…”

Section: Introductionmentioning

confidence: 99%

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Advances in the Micropropagation and Genetic Transformation of Abelmoschus Esculentus (L.) Moench for Insect Resistance

Daniel¹,

Duraipandiyan²,

Packiam³

2023

Curr Agri Res Jour

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Abelmoschus esculentus (L.) Moench, known as okra, is a common vegetable crop in many diets and serves as a nutrient-rich source. It has a high content of protein, vitamins, minerals and compounds of high medicinal value. India tops in the consumption of pods and ranks first among the worldwide total production. It is now widely cultivated in many countries. Among the factors that hamper okra's marketable fruit yield, insect pests are the major ones. As numerous pests attack vegetables, controlling insect pests is one of the key elements to improve the yield of this crop. A workable approach for improving okra yield is micropropagation. It has been employed for a variety of things, including as large multiplication, inducing somaclonal variation to improve the desirable agronomic traits, maintaining certain genotypes, and genetic modification utilising molecular techniques. In this review, we highlight the most significant research on the micropropagation of okra, which is mediated by a variety of regeneration responses. The media and growth regulators for each of the approaches discussed, we go through how transformation techniques for insect resistance have been made possible via micropropagation. Utilizing this technology might be a workable plan to add genes and enhance particular features. Studying molecular pathways is another option provided by genetic transformation. This offers benefits for developing breeding programmes and optimising field production especially the effective use of CRISPR in genetically diverse lepidopteran insects opened options to study gene functions, insect modification, and pest management.

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Section: A G R O B a C T E R I U M T U M E F A C I E N S -M E D I A T...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advances in the Micropropagation and Genetic Transformation of Abelmoschus Esculentus (L.) Moench for Insect Resistance

Daniel¹,

Duraipandiyan²,

Packiam³

2023

Curr Agri Res Jour

View full text Add to dashboard Cite

show abstract

Development of marker-free insect resistant transgenic okra (Abelmoschus esculentus L. Moench) expressing the cry1Ac gene and identification of vector backbone-free events

Cited by 1 publication

References 17 publications

Advances in the Micropropagation and Genetic Transformation of Abelmoschus Esculentus (L.) Moench for Insect Resistance

Advances in the Micropropagation and Genetic Transformation of Abelmoschus Esculentus (L.) Moench for Insect Resistance

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