Development of markers for the use of the PEF1 cytoplasm in sunflower hybrid breeding

Abstract

The use of the new cytoplasmic male sterility (CMS) source PEF1 in sunflower hybrid breeding requires markers closely linked to the restorer gene Rf_PEF1 necessary for fertility restoration of hybrids based on the PEF1 cytoplasm as well as diagnostic markers to distinguish the PEF1 cytoplasm from other cytoplasms. Bulked segregant analyses of 256 AFLP primer combinations identified 35 polymorphic primer combinations with 1-3 polymorphisms, resulting in 40 polymorphisms. Eighteen AFLP markers mapped together with the Rf_PEF1 gene covering 119.9 cM. The closest markers, E39M51_300R and E44M56_112A, mapped 3.9 and 6.0 cM to the Rf_PEF1 gene, respectively. Six SSR markers, which belong to the linkage group 13, were screened for polymorphisms between the parental lines. Only ORS630 was polymorphic, but did not map to the same linkage group as Rf_PEF1, indicating that Rf_PEF1 is not located on linkage group 13 where the restorer gene Rf1 for the PET1 cytoplasm is located. Diagnostic markers to distinguish the PEF1 cytoplasm from the PET1 and the fertile cytoplasm in sunflower were obtained using primer combinations for the atp9 gene and orfH522.

Comparative Analysis of the Complete Chloroplast Genome of the Alloplasmic Sunflower (Helianthus L.) Lines with Various CMS Types

“…By now, this type of CMS has been fairly well studied. In particular, its molecular genetic bases are known and STS markers have been developed for the mitochondrial orf522 gene, which make it possible to distinguish between fertile line and sterile line which contained CMS-PET1 (Schnabel et al, 2008). The molecular basis of the CMS types like PET2, ANN2 and MAX1 is insufficiently studied, which is an obstacle to their introduction into commercial breeding.…”

Sunflower Genetic Collection at the Vavilov Institute of Plant Industry

“…The collection at the fourth level is represented by lines with cms and pollen fertility restoration genes (Table 8). The atp9 and orfH522 molecular markers (Schnabel et al, 2008), which are specific for the mitochondrial genes associated with cms PET1, have been used to differentiate between the lines with fertile and sterile cytoplasm, as well as the difference between lines with cms PET1 (e.g. VIR 109,VIR 114,VIR 116,VIR 151,etc.…”

Registration of Cytoplasmic Male-Sterile Oilseed Sunflower Genetic Stocks, CMS GIG2 and CMS GIG2-RV, and Fertility Restoration Lines, RF GIG2-MAX 1631 and RF GIG2-MAX 1631-RV

“…Rf 4 is a single dominant gene that controls fertility restoration. Eight Rf genes have been mapped, including Rf 1 , Msc 1 , Rf 3 ‐RHA 340, Rf 3 ‐RHA 280, and Rf 5 – HA‐R9 for CMS PET‐1, Rf 4 for a new alloplasmic CMS GIG2, Rf ‐PEF1 for CMS PEF1, and Rf 6 from H. angustifolius L. for CMS 514A (Gentzbittel et al, 1995; Jan et al, 1998; Horn et al, 2003; Abratti et al, 2008; Feng and Jan, 2008; Schnabel et al, 2008; Yue et al, 2010; Liu et al, 2012; Qi et al, 2012; Liu et al, 2013; Qi and Seiler, 2013). Tightly linked simple sequence repeat (SSR) markers, ORS 13 and ORS 1114, mapped about 0.9 cM from the Rf 4 locus, and a dominant STS 10B1 mapped at 3.1 cM away will facilitate marker‐assisted selection in sunflower breeding (Feng and Jan, 2008).…”