Development of matrix solid-phase dispersion coupled with high-performance liquid chromatography for determination of jolkinolide A and jolkinolide B in Euphorbia fischeriana Steud.

Abstract

A novel and simple method was established for the extraction and determination of jolkinolide A and B in Euphorbia fischeriana Steud. using matrix solid-phase dispersion (MSPD) extraction and high-performance liquid chromatography (HPLC). The optimised conditions for the MSPD extraction were determined to be that silica gel was served as dispersant, the mass ratio of sample to silica gel was selected to be 1:4, and 5 mL of acetonitrile was used as elution solvent. The method exhibited a good performance in terms of linearity (r ≥ 0.9997) and the limits of detection in the range of 0.052-0.065 μg mL. The recoveries were in the range of 90.2-98.9% with relative standard deviations (RSDs) ranging from 1.3 to 3.5%. The extraction efficiencies obtained by the MSPD were higher than other extraction method with less cost of sample and solvent. At last, the optimised method was applied for analysing real samples.

Unprecedented diterpenoid dimers with soluble epoxide hydrolase inhibitory effect from Euphorbia fischeriana

“…Jolkinolide A is the major component in E. fischeriana produced by diterpene synthases from GGPP. 25,26 The hydration of Δ 11,12 in jolkinolide A could form intermediate i , which could be further oxidized to produce unit B. The hydrolysis of the lactone in jolkinolide A was able to obtain another intermediate ii , which could give iii by keto–enol tautomerism.…”

Optimization of extraction for Anemarrhena asphodeloides Bge. using silica gel‐based vortex‐homogenized matrix solid‐phase dispersion and rapid identification of antioxidant substances

“…It needs a great deal of organic reagent, which arouses environment pollution. Matrix solid‐phase dispersion (MSPD) is a relative sample preparation method in which sample homogenization, disruption, extraction, fractionation, and purification were simultaneously performed in one step and shorter extraction time was required [26]. Recently, many MSPD methods have been successfully used in the extraction of medicine and food [27–32].…”