Development of menaquinone-7 enriched nutraceutical: inside into medium engineering and process modeling

Abstract

Menaquinone 7 (MK-7) is nutritionally important metabolite found by fermentation mainly using B. subtilis species. In this study, soybean medium was modified to improve the MK-7 production using Bacillus subtilis NCIM 2708 under solid state fermentation. The objective of this study was to produce large amount of MK-7 within a short period of time. Nine nutritional components viz. glycerol, mannitol, dextrose, sucrose, yeast extract, malt extract, K 2 HPO 4 , MgSO 4 .7H 2 O and CaCl 2 were investigated to obtain the maximum MK-7 concentration. The highest MK-7 concentration 39.039 μg/g was obtained after 24 h of fermentation in the following optimised medium components: soybean 20 g, glycerol 40 ml/kg, mannitol 60 g/kg, yeast extract 4 g/kg, malt extract 8 g/kg and calcium chloride 4 g/kg. The maximum production of MK-7 56.757 μg/g was predicted by point prediction tool of Design Expert 7.1 software (Statease Inc. USA). This data shows 68.78 % validity of the predicted model.

Vitamin K as a Diet Supplement with Impact in Human Health: Current Evidence in Age-Related Diseases

“…Compared to chemical synthesis, biosynthetic production methods for natural vitamin K2 using bacterial fermentation have been the most studied and reported production systems for this vitamer [152][153][154]. This is mainly due to the advantage of selective production of the all-trans isomer by microorganisms, and the easy manipulation and culture conditions optimization of many bacterial strains.…”

Microbiome and Functional Analysis of a Traditional Food Process: Isolation of a Novel Species (Vibrio hibernica) With Industrial Potential

“…Vitamin K 2 has positive effects on preventing osteoporosis and cardiovascular disease, as well as aiding in blood coagulation (Mahdinia et al, 2017). Studies have already been employed to produce vitamin K 2 enriched products by traditional fermentation food processes (Mahanama et al, 2011;Singh et al, 2015). The vitamin B 12 is an important cofactor for many enzymes (Cheng et al, 2016;Bridwell-Rabb and Drennan, 2017).…”

Temperature‐induced mutagenesis‐based adaptive evolution of Bacillus amyloliquefaciens for improving the production efficiency of menaquinone‐7 from starch

“…can use mono-, diand polysaccharides besides glycerol, and these saccharides can be used as carbon sources for producing MK-7. 12,23,26,28 Starch was reported to have been used for MK-7 production by B. subtilis in previous research, but not enough to have a measurable effect on production efficiency of strain. 22,27 In our previous research, an MK-7-producing strain B. amyloliquefaciens Y-2 with ⊍-amylase activity was isolated from Chinese fermented bean.…”