Development of meridic and oligidic diets for rearing the aphid Myzus persicae

A chemically defined diet enables continuous rearing of the brown planthopper, Nilaparvata lugens(Stal)(Homoptera: Delphacidae).

“…However, the growth and reproduction of N. lugens on D-97 were still inferior to those on the susceptible rice variety TN1. This is consistent with some earlier studies regarding the rearing of other homopterans on holidic diets (Mitsuhashi, 1974;Mittler and Koski, 1976). Even Myzus persicae and Acyrthosiphon pisum, the most successful species reared on holidic diets in Homoptera, also showed lighter weight, slower development and lower fe-cundity when reared on chemically defined diets (Dadd and Mittler, 1966;Akey and Beck, 1972;van Emden, 1988).…”

Rearing of the aphid Myzus persicae on phloem exudate from Amsinckia douglasiana plants infected with curly top virus

“…adults was 289 lag on a 10% sucrose diet and 53 I lag on a 15% sucrose diet. These findings are in conformity with previous results of feeding M. persicae on diets with different sucrose concentrations (Mittler & Kleinjan, 1970) and on diets supplemented with yeast extract (Mittler & Koski, 1976). The mean weights of adults reared on healthy and curly top infected Amsinckia plants were 456 lag and 586 lag, respectively.…”

“…Droplets of exudation (10--50 tal) from both the upper and lower leaf surfaces were collected hourly by a capillary pipette. The exudate was pooled, stored at --12 °, and centrifuged at 3000 g for 5 min.Artificial diets with 10 or 15% sucrose and 0 or 2% yeast extract (based on formulations by Mitsuhashi, 1974, andKoski, 1976) and the exudate were sterilized by filtration through an 0.22 la Millipore filter, and 0.1 ml amounts encapsulated in UV sterilized, stretched membranes of Parafilm M (Mittler & Dadd, 1964). The sachets thus prepared were stored at -20 ° until they were presented to the aphids.One-day-old larvae, from a culture of M. persicae (a biotype from Yakima, Washington State) maintained on radish seedlings (Raphanus sativus var.…”

“…Artificial diets with 10 or 15% sucrose and 0 or 2% yeast extract (based on formulations by Mitsuhashi, 1974, andKoski, 1976) and the exudate were sterilized by filtration through an 0.22 la Millipore filter, and 0.1 ml amounts encapsulated in UV sterilized, stretched membranes of Parafilm M (Mittler & Dadd, 1964). The sachets thus prepared were stored at -20 ° until they were presented to the aphids.…”

An Artificial Diet for the Sugarcane Aphid (Melanaphis sacchariZehntner) (Hemiptera: Aphididae) with Potential Uses for in Vitro Toxicological Studies

“…One diet we evaluated was originally developed for Myzus persicae Sulzer (Hemiptera: Aphididae) (Mittler & Koski 1976) and consisted of 15% sucrose (Reasol, Iztapalapa, Mexico City, Mexico); 2.5 g casein hydrolysate (Sigma-Aldrich, 22090-500G, St. Louis, Missouri, USA); 2% yeast extract (MCD Lab, Tlalnepantla, Mexico City Mexico, Mexico); 123 mg MgSO 4 7H 2 O (Jalmek, San Nicolas de los Garza, Nuevo Leon, Mexico); 100 mg ascorbic acid (Sigma-Aldrich, A0278-25G, St. Louis, Missouri, USA); 10 mg niacin (Sigma-Aldrich, N0761-100G, St. Louis, Missouri, USA); 5 mg calcium pantothenate (Sigma-Aldrich, C8731-25G, St. Louis, Missouri, USA); 2.5 mg pyridoxine (Sigma-Aldrich, P5669-5G, St. Louis, Missouri, USA); 2.5 mg thiamine (Sigma-Aldrich, T1270-25G, St. Louis, Missouri, USA); and 1.5 mg K 2 HPO 4 3H 2 O (Jalmek, San Nicolas de los Garza, Nuevo Leon, Mexico).…”