Development of Metarhizium humberi in Aedes aegypti eggs

Sousa, N.A.; Rodrigues, Juliana; Arruda, Walquíria; Humber, Richard A.; Luz, Christian

doi:10.1016/j.jip.2021.107648

Cited by 8 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most Metarhizium were reported to form clearly defined appressoria, which produce hyphae that achieve penetration via a combination of mechanical pressure and hydrolytic enzymes (Ortiz-Urquiza and Keyhani, 2013Keyhani, , 2016. For instance, Metarhizium anisopliae and Metarhizium humberi conidia form short germ tubes, which swell to grow appressoria at the tips (St Leger et al, 1989;de Sousa et al, 2021). It has been reported that appressoria are essential for the invasion of most entomopathogenic fungi (Lin et al, 2011;Butt et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The conidia of Metarhizium spp. and Beauveria bassiana could germinate and differentiate appressoria before breaching the insect tegument (Leger et al, 1987;St Leger et al, 1989;Wang and St Leger, 2005;Güerri-Agulló et al, 2010;Huang et al, 2015;de Sousa et al, 2021). Nevertheless, appressoria are unnecessary for entomopathogenic fungi to penetrate insect cuticles.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The invasion process of the entomopathogenic fungus Ophiocordyceps sinensis into the larvae of ghost moths (Thitarodes xiaojinensis) using a GFP-labeled strain

Qin

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Chinese cordyceps is a well-known and valuable traditional Chinese medicine that forms after Ophiocordyceps sinensis parasitizes ghost moth larvae. The low natural infection rate of O. sinensis limits large-scale artificial cultivation of Chinese cordyceps, and the invasion process is unclear. To investigate the temporal and spatial regulation when O. sinensis enters ghost moths, we constructed an O. sinensis transformant that stably expresses green fluorescent protein (GFP). Inoculating Thitarodes xiaojinensis larvae with a high concentration of GFP-labeled O. sinensis, we observed that O. sinensis conidia could adhere to the host cuticle within 2 days, germinate penetration pegs within 4 days, and produce blastospores in the host hemocoel within 6 days. The reconstructed three-dimensional (3D) structures of the invasion sites showed that penetration pegs germinated directly from O. sinensis conidia at the joining site with the larval cuticle. Differentiated appressoria or hyphae along the host epicuticle are not required for O. sinensis to invade ghost moths. Overall, the specific invasion process of O. sinensis into its host is clarified, and we provided a new perspective on the invasion process of entomopathogenic fungi.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The invasion process of the entomopathogenic fungus Ophiocordyceps sinensis into the larvae of ghost moths (Thitarodes xiaojinensis) using a GFP-labeled strain

Qin

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…They then penetrate the chorion of egg, resulting in the growth of mycelia and new conidia on the egg surfaces. This growth disrupts the eggshells and halts the development of the embryo within the eggs [29]. In some cases, fully developed larvae in the eggs may be stimulated to eclose prematurely when exposed to conidia or when invaded by hyphae.…”

Section: Invasion Through Mosquito Cuticlementioning

confidence: 99%

Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol

Qin

Liu

Peng

et al. 2023

JoF

View full text Add to dashboard Cite

Fungal diseases are widespread among insects and play a crucial role in naturally regulating insect populations. Mosquitoes, known as vectors for numerous infectious diseases, pose a significant threat to human health. Entomopathogenic fungi (EPF) have emerged as highly promising alternative agents to chemical mosquitocides for controlling mosquitoes at all stages of their life cycle due to their unique infection pathway through direct contact with the insect’s cuticle. In recent years, significant advancements have been made in understanding the infection pathways and pathogenic mechanisms of EPF against mosquitoes. Various strategies involving the use of EPF alone or combinations with other approaches have been employed to target mosquitoes at various developmental stages. Moreover, the application of genetic technologies in fungi has opened up new avenues for enhancing the mosquitocidal efficacy of EPF. This review presents a comprehensive summary of recent advancements in our understanding the pathogenic mechanisms of EPF, their applications in mosquito management, and the combination of EPF with other approaches and employment of transgenic technologies. The biosafety concerns associated with their use and the corresponding approaches are also discussed. The recent progress suggests that EPF have the potential to serve as a future biorational tool for controlling mosquito vectors.

show abstract

“…[20][21][22][23][24][25] Laboratory evaluation showed that B. bassiana strain ARP14 was virulent against G. molesta first instar larvae and adults, 26 and recent work has illustrated its pathogenicity to insect eggs, which it can infect through chorion in a process similar to those used to penetrate the cuticle. [27][28][29][30] Therefore, we explored the compatibility of Trichogramma parasitoids and B. bassiana for potential combined application to enhance the integrated management of G. molesta.…”

Section: Introductionmentioning

confidence: 99%

“…The generalist EPF Beauveria bassiana (Balsamo) has been widely used in vegetable and orchard pest control via direct application in the field and also via parasitoid‐mediated dissemination 20–25 . Laboratory evaluation showed that B. bassiana strain ARP14 was virulent against G. molesta first instar larvae and adults, 26 and recent work has illustrated its pathogenicity to insect eggs, which it can infect through chorion in a process similar to those used to penetrate the cuticle 27–30 . Therefore, we explored the compatibility of Trichogramma parasitoids and B. bassiana for potential combined application to enhance the integrated management of G. molesta .…”

Section: Introductionmentioning

confidence: 99%

Laboratory evaluation of the compatibility of Beauveria bassiana with the egg parasitoid Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae) for joint application against the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Fang

Liu

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND The entomopathogenic fungus Beauveria bassiana and the egg parasitoid Trichogramma dendrolimi can both contribute to biological control of the global fruit borer Grapholita molesta. To derive insights for optimizing their combined application in augmentation programs, we assayed fungal pathogenicity to both G. molesta (eggs, larvae and adults) and adult parasitoids, and assessed wasp acquisition and transmission of fungal spores following their emergence from B. bassiana‐treated host eggs. RESULTS Concentrations of 108 conidia mL−1 of B. bassiana and higher caused virtually complete mortality of G. molesta larvae, but less than 30% mortality of eggs, and female moths surviving treatments had reduced fecundity. More than 80% of adult T. dendrolimi survived B. bassiana treatments of 107 conidia mL−1, and more than 60% survived 109 conidia mL−1, with surviving females achieving reproductive success equivalent to controls. Parasitoid females preferred healthy eggs over B. bassiana‐infected ones in choice tests; wasps emerging from treated eggs carried about 104 conidia each and contributed an additional 11% host mortality in trials combining both agents. CONCLUSION Our results indicate that combined applications of B. bassiana and T. dendrolimi can have complimentary impacts on G. molesta, the wasps compensating for low fungal pathogenicity to eggs and their progeny potentially aiding in subsequent disease transmission. Although foraging wasps tended to avoid infected eggs, negative interactions between the two agents might be further mitigated by timing B. bassiana applications from late instar larva to early moth stage, and T. dendrolimi releases several days later to coincide with peak oviposition periods. © 2022 Society of Chemical Industry.

show abstract

Development of Metarhizium humberi in Aedes aegypti eggs

Cited by 8 publications

References 41 publications

The invasion process of the entomopathogenic fungus Ophiocordyceps sinensis into the larvae of ghost moths (Thitarodes xiaojinensis) using a GFP-labeled strain

The invasion process of the entomopathogenic fungus Ophiocordyceps sinensis into the larvae of ghost moths (Thitarodes xiaojinensis) using a GFP-labeled strain

Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol

Laboratory evaluation of the compatibility of Beauveria bassiana with the egg parasitoid Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae) for joint application against the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Contact Info

Product

Resources

About